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Literature summary for 3.1.3.2 extracted from

  • Malveaux, F.J.; San Clemente, C.L.
    Staphylococcal acid phosphatase: preliminary physical and chemical characterization of the loosely bound enzyme (1969), J. Bacteriol., 97, 1215-1219.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
mercaptoethanol stimulates Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Staphylococcus aureus
F-
-
Staphylococcus aureus
Hg2+
-
Staphylococcus aureus
iodoacetate
-
Staphylococcus aureus
molybdate
-
Staphylococcus aureus
Pb2+
-
Staphylococcus aureus
phosphate
-
Staphylococcus aureus
Tartrate tartrate has no effect Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
p-nitrophenyl phosphate
-
Staphylococcus aureus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ stimulates Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
PS 55
-
Staphylococcus aureus PS 55
-
PS 55
-

Purification (Commentary)

Purification (Comment) Organism
-
Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O 10.4% of the activity with p-nitrophenyl phosphate Staphylococcus aureus AMP + phosphate
-
?
alpha-glycerophosphate + H2O
-
Staphylococcus aureus glycerol + phosphate
-
?
alpha-glycerophosphate + H2O
-
Staphylococcus aureus PS 55 glycerol + phosphate
-
?
D-fructose 1,6-diphosphate + H2O 13.8% of the activity with p-nitrophenyl phosphate Staphylococcus aureus ?
-
?
D-fructose 1,6-diphosphate + H2O 13.8% of the activity with p-nitrophenyl phosphate Staphylococcus aureus PS 55 ?
-
?
D-fructose 6-phosphate + H2O 38% of the activity with p-nitrophenyl phosphate Staphylococcus aureus D-fructose + phosphate
-
?
D-fructose 6-phosphate + H2O 38% of the activity with p-nitrophenyl phosphate Staphylococcus aureus PS 55 D-fructose + phosphate
-
?
GDP + H2O 17.3% of the activity with p-nitrophenyl phosphate Staphylococcus aureus GMP + phosphate
-
?
Glucose 6-phosphate + H2O 3.5% of the activity with p-nitrophenyl phosphate Staphylococcus aureus Glucose + phosphate
-
?
Glucose 6-phosphate + H2O 3.5% of the activity with p-nitrophenyl phosphate Staphylococcus aureus PS 55 Glucose + phosphate
-
?
glyceraldehyde 3-phosphate + H2O 82.8% of the activity with p-nitrophenyl phosphate Staphylococcus aureus glyceraldehyde + phosphate
-
?
p-nitrophenyl phosphate + H2O
-
Staphylococcus aureus p-nitrophenol + phosphate
-
?
p-nitrophenyl phosphate + H2O
-
Staphylococcus aureus PS 55 p-nitrophenol + phosphate
-
?
phenolphthalein monophosphate + H2O 24.1% of the activity with p-nitrophenyl phosphate Staphylococcus aureus phenolphthalein + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
49
-
-
Staphylococcus aureus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
most stable in alkaline pH range, pH 8.5-9.5 at temperatures below 50°C Staphylococcus aureus
70
-
complete inactivation after 5 min in absence of Cu2+ Staphylococcus aureus
80
-
5 min, complete inactivation in presence of Cu2+ Staphylococcus aureus

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5 9.5 most stable in alkaline pH range, pH 8.5-9.5 at temperatures below 50°C Staphylococcus aureus