Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-2-hydroxymethyl-1,3-propanediol | - |
Pseudomonas aeruginosa | |
2-amino-2-methyl-1,3-propanediol | - |
Pseudomonas aeruginosa | |
2-amino-2-methyl-1-propanol | - |
Pseudomonas aeruginosa | |
alaninol | - |
Pseudomonas aeruginosa | |
additional information | no or poor inhibition by DL-serine and D-alanine, and by 1-propanol, 3-methyl-1-butanol, 3-methyl-1-butanol, and choline, enzyme inhibition is more effective with N-trimethylated compounds than with N-triethylated ones | Pseudomonas aeruginosa | |
N-tetraethylammonium | - |
Pseudomonas aeruginosa | |
N-tetramethylammonium | - |
Pseudomonas aeruginosa | |
N-trimethylammonium | - |
Pseudomonas aeruginosa | |
serinol | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Pseudomonas aeruginosa | |
0.35 | - |
Mg2+ | pH 5.0, 37°C, partially purified enzyme | Pseudomonas aeruginosa | |
2 | - |
4-nitrophenyl phosphate | pH 5.0, 37°C, partially purified enzyme | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Pseudomonas aeruginosa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Pseudomonas aeruginosa | |
additional information | no effect by NH4Cl, LiCl, and KCl | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially by preparation of periplasmic fraction including MgCl2 treatment and ammonium sulfate precipitation, co-purification of cholinesterase, which is removed by gel filtration | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
partially purified enzyme | Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
4°C, partially purified enzyme, precipitated by 90% ammonium sulfate, in Tris-HCl, pH 7.4, stable for at least 3 months | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Pseudomonas aeruginosa | |
0.6 | - |
N-tetramethylammonium | pH 5.0, 37°C, partially purified enzyme | Pseudomonas aeruginosa | |
4.2 | - |
N-tetraethylammonium | pH 5.0, 37°C, partially purified enzyme | Pseudomonas aeruginosa |