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Literature summary for 3.1.3.2 extracted from

  • Garrido, M.N.; Lisa, T.A.; Domenech, C.E.
    Pseudomonas aeruginosa acid phosphatase contains an anionic site with a trimethyl subsite. Kinetic evidence obtained with alkylammonium ions (1988), Mol. Cell. Biochem., 84, 41-49.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-amino-2-hydroxymethyl-1,3-propanediol
-
Pseudomonas aeruginosa
2-amino-2-methyl-1,3-propanediol
-
Pseudomonas aeruginosa
2-amino-2-methyl-1-propanol
-
Pseudomonas aeruginosa
alaninol
-
Pseudomonas aeruginosa
additional information no or poor inhibition by DL-serine and D-alanine, and by 1-propanol, 3-methyl-1-butanol, 3-methyl-1-butanol, and choline, enzyme inhibition is more effective with N-trimethylated compounds than with N-triethylated ones Pseudomonas aeruginosa
N-tetraethylammonium
-
Pseudomonas aeruginosa
N-tetramethylammonium
-
Pseudomonas aeruginosa
N-trimethylammonium
-
Pseudomonas aeruginosa
serinol
-
Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Pseudomonas aeruginosa
0.35
-
Mg2+ pH 5.0, 37°C, partially purified enzyme Pseudomonas aeruginosa
2
-
4-nitrophenyl phosphate pH 5.0, 37°C, partially purified enzyme Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Pseudomonas aeruginosa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Pseudomonas aeruginosa
additional information no effect by NH4Cl, LiCl, and KCl Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially by preparation of periplasmic fraction including MgCl2 treatment and ammonium sulfate precipitation, co-purification of cholinesterase, which is removed by gel filtration Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1
-
partially purified enzyme Pseudomonas aeruginosa

Storage Stability

Storage Stability Organism
4°C, partially purified enzyme, precipitated by 90% ammonium sulfate, in Tris-HCl, pH 7.4, stable for at least 3 months Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Pseudomonas aeruginosa 4-nitrophenol + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics Pseudomonas aeruginosa
0.6
-
N-tetramethylammonium pH 5.0, 37°C, partially purified enzyme Pseudomonas aeruginosa
4.2
-
N-tetraethylammonium pH 5.0, 37°C, partially purified enzyme Pseudomonas aeruginosa