Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | phosphate starvation induces the enzyme activity 4fold | Solanum lycopersicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 99% inhibition at 10 mM | Solanum lycopersicum | |
Fe3+ | complete inhibition at 10 mM | Solanum lycopersicum | |
fluoride | mixed type | Solanum lycopersicum | |
molybdate | competitive | Solanum lycopersicum | |
additional information | the purple enzyme is insensitive to L-tartrate | Solanum lycopersicum | |
phosphate | mixed type | Solanum lycopersicum | |
vanadate | mixed type | Solanum lycopersicum | |
Zn2+ | 88% inhibition at 10 mM | Solanum lycopersicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Solanum lycopersicum | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates 150% at 10 mM | Solanum lycopersicum | |
Mn2+ | activates 130% at 10 mM | Solanum lycopersicum | |
additional information | the enzyme is not affected by BaCl2, CaCl2, CoCl2, KCl, NaCl, or EDTA | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE | Solanum lycopersicum |
63000 | - |
1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE | Solanum lycopersicum |
142000 | - |
native PAGE | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Solanum lycopersicum | the enzyme scavenges phosphate from intracellular phosphate esters and might be involved in oxidative reagents metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Solanum lycopersicum |
Purification (Comment) | Organism |
---|---|
intracellular enzyme 476fold to homogeneity from phosphate-starved cell culture by PEG 8000 treatment, adsorption chromatography, gel filtration, and concanavalin A affinity chromatography | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | phosphate-starved | Solanum lycopersicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
620 | - |
purified enzyme | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Solanum lycopersicum | 4-nitrophenol + phosphate | - |
? | |
additional information | the enzyme scavenges phosphate from intracellular phosphate esters and might be involved in oxidative reagents metabolism | Solanum lycopersicum | ? | - |
? | |
additional information | substrate specificity, overview, the enzyme shows broad substrate specificity and exhibits peroxidase activity, EC 1.11.1.7, optimal at pH 9.0 and insensitive to Mg2+ and molydate | Solanum lycopersicum | ? | - |
? | |
phosphoenolpyruvate + H2O | best substrate | Solanum lycopersicum | pyruvate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 63000, alpha-subunit, + 1 * 57000, beta-subunit, SDS-PAGE | Solanum lycopersicum |
More | peptide mapping | Solanum lycopersicum |
Synonyms | Comment | Organism |
---|---|---|
IAP | - |
Solanum lycopersicum |
intracellular acid phosphatase | - |
Solanum lycopersicum |
PAP | - |
Solanum lycopersicum |
purple acid phosphatase | - |
Solanum lycopersicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
loss of 20% activity after 5 min | Solanum lycopersicum |
65 | - |
loss of 50% activity after 5 min | Solanum lycopersicum |
70 | - |
loss of 80% activity after 5 min | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.1 | - |
- |
Solanum lycopersicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.8 | 6.5 | - |
Solanum lycopersicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
molybdate | pH 5.1 | Solanum lycopersicum | |
0.0065 | - |
vanadate | pH 5.1 | Solanum lycopersicum | |
0.094 | - |
fluoride | pH 5.1 | Solanum lycopersicum | |
1.6 | - |
phosphate | pH 5.1 | Solanum lycopersicum |