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Literature summary for 3.1.3.2 extracted from

  • Himeno, M.; Koutoku, H.; Ishikawa, T.; Kato, K.
    Acid phosphatase in rat liver lysosomal membranes: purification and characterization (1989), J. Biochem., 105, 449-456.
    View publication on PubMed
Search for more literature for 3.1.3.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 p-nitrophenyl phosphate
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome membrane Rattus norvegicus 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 4 * 67000, SDS-PAGE Rattus norvegicus
320000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein membrane enzyme contains 24.3% carbohydrate consisting of mannose, galactose, fucose, N-acetylglucosamine, N-acetylgalactosamine and N-acetylneuraminic acid in a molar ratio of 38:20:5:36:4:11 respectively Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
membrane enzyme M-APase and three soluble enzyme forms: C-APase I, II and III Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
241
-
 membrane enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl phosphate + H2O
-
 Rattus norvegicus p-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 67000, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 hydrolysis of p-nitrophenyl phosphate Rattus norvegicus