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Literature summary for 3.1.3.2 extracted from

  • Waratrujiwong, T.; Krebs, B.; Spener, F.; Visoottiviseth, P.
    Recombinant purple acid phosphatase isoform 3 from sweet potato is an enzyme with a diiron metal center (2006), FEBS J., 273, 1649-1659.
    View publication on PubMed
Search for more literature for 3.1.3.2

Activating Compound

Activating Compound Comment Organism Structure
L-ascorbic acid activates Ipomoea batatas

Cloned(Commentary)

Cloned (Comment) Organism
subcloning in Escherichia coli, expression of wild-type and mutant isozyme 3 in Spodoptera frugiperda Sf9 cells using the baculovirus infection system Ipomoea batatas

Protein Variants

Protein Variants Comment Organism
Y291A site-directed mutagenesis of an substrate binding ssite residue to the correspondent residue of isozyme 1, the mutant shows decreased activity compared to the wild-type isozyme 3 Ipomoea batatas
Y291H site-directed mutagenesis of an substrate binding ssite residue to the correspondent residue of isozyme 1, the mutant shows increased activity compared to the wild-type isozyme 3 Ipomoea batatas

Inhibitors

Inhibitors Comment Organism Structure
Mn2+
-
 Ipomoea batatas
Zn2+
-
 Ipomoea batatas

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ isozyme 3 contains a diiron metal center in contrast to isozymes 1 and 2 which contain Fe(III)-Mn(II) centers, analysis of prosthetic metal ions/groups Ipomoea batatas

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 2 * 55000, isozyme 3, SDS-PAGE Ipomoea batatas
110000
-
 isozyme 3, gel filtration Ipomoea batatas

Organism

Organism UniProt Comment Textmining
Ipomoea batatas Q9ZS50 isozyme 3
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isozyme 3 199fold from Sf9 insect cells in two steps by QBB-resin and hydrophobic interaction chromatography Ipomoea batatas

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
321.8
-
 purified recombinant wild-type enzyme Ipomoea batatas
545
-
 purified recombinant wild-type enzyme in presence of L-ascorbic acid at 10 mM Ipomoea batatas

Subunits

Subunits Comment Organism
dimer 2 * 55000, isozyme 3, SDS-PAGE Ipomoea batatas

Synonyms

Synonyms Comment Organism
PAP
-
 Ipomoea batatas
purple acid phosphatase
-
 Ipomoea batatas

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Ipomoea batatas

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Ipomoea batatas