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Literature summary for 3.1.3.2 extracted from

  • Souza, A.A.; Leitao, V.O.; Ramada, M.H.; Mehdad, A.; Georg, R.d.e. .C.; Ulhoa, C.J.; de Freitas, S.M.
    Trichoderma harzianum produces a new thermally stable acid phosphatase, with potential for biotechnological application (2016), PLoS ONE, 11, e0150455 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.2

Activating Compound

Activating Compound Comment Organism Structure
EDTA 137.6% activity at 5 mM Trichoderma harzianum
sodium tartrate 114.4% activity at 5 mM Trichoderma harzianum

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 93.5% residual activity at 5 mM Trichoderma harzianum
phosphate 33% residual activity at 5 mM Trichoderma harzianum
tungstate 1% residual activity at 5 mM Trichoderma harzianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000027
-
 4-nitrophenyl phosphate at pH 3.8 and 65°C Trichoderma harzianum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not require metal divalent ions Trichoderma harzianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
326000
-
 dynamic light scattering Trichoderma harzianum

Organism

Organism UniProt Comment Textmining
Trichoderma harzianum
-
-
-
Trichoderma harzianum ALL42
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Trichoderma harzianum

Purification (Commentary)

Purification (Comment) Organism
HiTrap phenyl column chromatography and Superdex 200 gel filtration Trichoderma harzianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O 100% activity Trichoderma harzianum 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O 100% activity Trichoderma harzianum ALL42 4-nitrophenol + phosphate
-
 ?
ADP + H2O 39% activity compared to 4-nitrophenyl phosphate Trichoderma harzianum AMP + phosphate
-
 ?
AMP + H2O 30% activity compared to 4-nitrophenyl phosphate Trichoderma harzianum adenosine + phosphate
-
 ?
AMP + H2O 30% activity compared to 4-nitrophenyl phosphate Trichoderma harzianum ALL42 adenosine + phosphate
-
 ?
ATP + H2O 25.3% activity compared to 4-nitrophenyl phosphate Trichoderma harzianum ADP + phosphate
-
 ?
ATP + H2O 25.3% activity compared to 4-nitrophenyl phosphate Trichoderma harzianum ALL42 ADP + phosphate
-
 ?
beta-D-glucose 1-phosphate + H2O 35.5% activity compared to 4-nitrophenyl phosphate Trichoderma harzianum beta-D-glucose + phosphate
-
 ?
D-fructose 6-phosphate + H2O 33% activity compared to 4-nitrophenyl phosphate Trichoderma harzianum D-fructose + phosphate
-
 ?
D-fructose 6-phosphate + H2O 33% activity compared to 4-nitrophenyl phosphate Trichoderma harzianum ALL42 D-fructose + phosphate
-
 ?
D-glucose 1-phosphate + H2O 49.4% activity compared to 4-nitrophenyl phosphate Trichoderma harzianum D-glucose + phosphate
-
 ?
phenyl phosphate + H2O 52.3% activity compared to 4-nitrophenyl phosphate Trichoderma harzianum phenol + phosphate
-
 ?
phenyl phosphate + H2O 52.3% activity compared to 4-nitrophenyl phosphate Trichoderma harzianum ALL42 phenol + phosphate
-
 ?
phytic acid + H2O 20.5% activity compared to 4-nitrophenyl phosphate Trichoderma harzianum ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 90000, SDS-PAGE Trichoderma harzianum

Synonyms

Synonyms Comment Organism
ACPase II
-
 Trichoderma harzianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Trichoderma harzianum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 70 about 30% activity at 35°C, about 40% activity at 40°C, about 60% activity at 45-50°C, about 65% activity at 55°C, about 92% activity at 60°C, 100% activity at 65°C, about 96% activity at 70°C, less than 10% activity at 80°C Trichoderma harzianum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 the enzyme is stable at 55°C for 120 min, retaining 60% of its activity. The enzyme shows 61%, 48% and 27% activity after 60 min at 50°C, 55°C, and 60°C, respectively Trichoderma harzianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8
-
-
 Trichoderma harzianum

pH Range

pH Minimum pH Maximum Comment Organism
3.2 5.2 more than 70% activity between pH 3.2 and 5.2 Trichoderma harzianum