Application | Comment | Organism |
---|---|---|
synthesis | immobilization of enzyme onto glutaraldehyde activated chitosan beads. Chitosan beads activated with 2% of glutaraldehyde demonstrate maximum immobilization yield of about 83%. The immobilized enzyme shows optimum activity at pH 7.0, while the soluble form is maximally active at pH 5.0. Both soluble and immobilized acid phosphatase exhibit maximum activity at 60°C. The immobilization on chitosan beads enhances the shelf life of acid phosphatase. The immobilized enzyme retains its more than 50% hydrolytic activity for approximately two months and can be reused for more than 40 cycles of reaction | Vigna aconitifolia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
4-nitrophenyl phosphate | pH 5.0, 37°C, enzyme immobilized onto chitosan beads | Vigna aconitifolia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna aconitifolia | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Vigna aconitifolia | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate | - |
Vigna aconitifolia | 4-nitrophenol + phosphate | - |
? |