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Information on EC 3.4.21.79 - granzyme B
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3.4.21.79 granzyme BSpecify your search results
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- 3.4.21.79
- perforin
- t-cells
- cd8
-
cytolytic
-
immunotherapy
- vaccine
- lymphoma
-
rejection
- tnf
- dendritic
-
cell-mediated
- ifn-gamma
-
allograft
-
tregs
- tia-1
- fasl
- foxp3
-
autologous
- interferon-gamma
-
caspases
-
degranulation
-
allogeneic
-
epstein-barr
-
antigen-specific
-
virus-specific
-
immunophenotype
-
ctla-4
-
anti-cd3
-
tumor-infiltrating
-
nk-cell
-
extranodal
-
elispot
- synthesis
-
eomes
-
graft-versus-leukemia
-
cd45ro
-
alcls
- biotechnology
-
death-1
- analysis
-
intragraft
- lag-3
-
hiv-specific
- medicine
- diagnostics
- degradation
-
alloreactive
-
lymphocyte-mediated
-
lymphokine-activated
- crma
-
b-mediated
- pharmacology
-
ag-specific
-
banff
-
hepatosplenic
-
anti-pd-1
- interleukin-15
The enzyme appears in viruses and cellular organisms
Synonyms
granzyme b, gra-b, asp-ase, lymphocyte protease, gzm b, granzyme g, pro-apoptotic serine protease, ctla1, progrb, cytotoxic cell proteinase-1, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Asp-ase
C11
-
-
-
-
CCP1
-
-
-
-
CCPII
-
-
-
-
CTLA1
-
-
-
-
CTSGL1
-
-
-
-
Cytotoxic cell proteinase-1
-
-
-
-
cytotoxic serine protease granzyme B
granzyme B
Granzyme G
-
-
-
-
Granzyme H
GrB
GrzmB
GzmB
HLp
-
-
-
-
Human lymphocyte protein
-
-
-
-
Lymphocyte protease
-
-
-
-
proGrB
Proteinase, CCP1
-
-
-
-
SECT
-
-
-
-
T-cell serine protease 1-3E
-
-
-
-
granzyme B
-
-
Granzyme H
-
-
-
-
GrB
-
-
GrB
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
preferential cleavage: -Asp-/- >> -Asn-/- > -Met-/-, -Ser-/-
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
-
-
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CAS REGISTRY NUMBER
COMMENTARY
143180-74-9
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-aminobenzoyl-IEPDSSMESK-dinitrophenyl + H2O
?
-
susbtrate is specific for human GrB
-
-
?
Ace-Ile-Glu-Pro-Asp-p-nitroanilide + H2O
Ace-Ile-Glu-Pro-Asp + p-nitroaniline
-
-
-
-
?
acetyl-DEVD-7-amido-4-methylcoumarin + H2O
?
-
efficient cleavage by GzmB after K562 cells are exposed to sublytic perforin or streptolysin O. GzmH shows only baseline readings
-
-
?
acetyl-IAPD-7-amido-4-methylcoumarin + H2O
acetyl-IAPD + 7-amino-4-methylcoumarin
-
-
-
?
acetyl-IEFD-7-amido-4-methylcoumarin + H2O
acetyl-IEFD + 7-amino-4-methylcoumarin
-
-
-
?
acetyl-IEPD-7-amido-4-methylcoumarin + H2O
acetyl-IEPD + 7-amino-4-methylcoumarin
-
-
-
?
acetyl-IKPD-7-amido-4-methylcoumarin + H2O
acetyl-IKPD + 7-amino-4-methylcoumarin
-
-
-
?
acetyl-LEFD-7-amido-4-methylcoumarin + H2O
acetyl-LEFD + 7-amino-4-methylcoumarin
-
-
-
?
acetyl-LEPD-7-amido-4-methylcoumarin + H2O
acetyl-LEPD + 7-amino-4-methylcoumarin
-
-
-
?
acetyl-YVAD-4-nitroanilide + H2O
acetyl-YVAD + 4-nitroaniline
-
an asp-ase substrate
-
-
?
acetylcholine receptor
?
-
granzyme B efficiently and specifically cleaves alpha and epsilon subunits of acetylcholine receptor, especially the epsilon subunit
-
-
?
acetylcholine receptor + H2O
?
-
extracellular GrB substrate. Implication: cleavage results in a reduction of the receptor in neuromuscular junctions and yields an autoantigenic fragment
-
-
?
adenovirus DNA-binding protein + H2O
?
-
gzmB and gzmH. Direct cleavage of DNA-binding protein by gzmH is a critical component of the cytotoxic antiviral response against adenovirus, which slows down DNA viral replication. Cleaved by gzmH at multiple sites, most efficient cleavage at Met118, when this site is mutated, gzmH instead cleaves at Phe121
-
-
?
aggrecan + H2O
?
-
extracellular GrB substrate. Implication: Disruption of structural integrity in cartilage
-
-
?
AHNAK + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: calcium signalling, associated disease: systemic lupus erythematosus
-
-
?
alanyl tRNA synthetase + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: translation, associated disease: myositis
-
-
?
alpha-fodrin + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: cytoskeletal protein, associated disease: Sjörgen's syndrome
-
-
?
alpha-tubulin + H2O
truncated alpha-tubulin
-
alpha-tubulin derived from HeLa S100 extracts or Jurkat S100 extracts is cleaved by grB in a caspase-independent manner. Cleavage occurs at D438. Polymerized and soluble alpha-tubulin can be cleaved
-
-
?
ATSY-7-amido-4-methylcoumarin + H2O
ATSY + 7-amino-4-methylcoumarin
-
GzmH
-
-
?
Bcl-2-associated athano gene-1 + H2O
?
-
GrB proteolysis is independent of caspase activity
-
-
?
BCL2/adenovirus E1B 19 kDa protein-interacting protein 2 + H2O
?
-
generated with similar efficiency by mouse and human recombinant granzyme B
-
-
?
BCL2/adenovirus E1B 19-kDa protein-interacting protein 2 + H2O
?
-
generated with similar efficiency by mouse and human recombinant granzyme B
-
-
?
beta-actin + H2O
?
-
GrB cleavage fragments of beta-actin are detected in medullary carcinoma of the breast tumor lysates, suggesting that CTL-mediated death of medullary carcinoma of the breast tumor cells and actin redistribution may have a function in the generation of autoimmunity to beta-actin
-
-
?
cartilage proteoglycans + H2O
?
-
extracellular GrB substrate. Implication: Disruption of structural integrity in cartilage
-
-
?
caspase-8 + H2O
?
-
cleaves human caspase-8 but fails to cleave the mouse counterpart of caspase-8. Mouse caspase-8 is processed only upon addition of cytochrome c and dATP to J774 extracts to activate the apoptosome pathway to caspase activation
-
-
?
centromere protein B + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: mitosis, associated disease: Scleroderma
-
-
?
centromere protein C + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: mitosis, associated disease: Scleroderma
-
-
?
chromodomain helicase DNA binding 4 + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: gene expression, associated disease: myositis
-
-
?
DNA binding protein + H2O
?
-
granzyme B and granzyme H (cleavage at Met118 and Phe121)
-
-
?
DNA-PK catalytic subunit + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: DNA repair, associated disease: myositis
-
-
?
FGFR1 + H2O
?
-
extracellular GrB substrate. Implication: cleavage activates pro-cell death functions as well as inactivates pro-growth signals
-
-
?
glutamate receptor subunit 3 + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: Glutamate receptor, associated disease: Rasmussen's encephalitis
-
-
?
hepatitis antigen lamin B + H2O
?
-
cleavage of autoimmune hepatitis antigen lamin B by GrA and B causes disruption of the nuclear lamina, by uncoupling lamin B from its nuclear localisation signal
-
-
?
histidyl tRNA synthetase + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: translation, associated disease: myositis
-
-
?
Hsc70/Hsp70-interacting protein + H2O
?
-
Hsc70/Hsp70-interacting protein is cleaved at both GrB cleavage sites (at sequences IEPD92-TD and INPD180-SA) during NK-mediated cell death in a caspase-independent manner. Hsc70/Hsp70-interacting protein is a substrate unique to GrB
-
-
?
Hsp90 + H2O
?
-
is proteolyzed at multiple sites by GrB. Cleavage at sequences IDED693-EV and INPD631-PI in Hsp90beta. Cleavage at sequences IDED701-DP, INPD639-HS and VRTD175-TG in Hsp90alpha
-
-
?
human endogenous retrovirus K-10 gag + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: endogenous retrovirus, associated disease: systemic lupus erythematosus
-
-
?
ICAD + H2O
?
-
cleaves human ICAD but fails to cleave the mouse counterpart
-
-
?
inhibitor of caspase-activated DNase + H2O
caspase-activated DNase
-
cleaved in a dose-dependent fashion
-
-
?
interleukin IL-1alpha + H2O
?
-
cleavage after the motif IAND103, no substrate for caspases -1, -3, -4, -5, and -7
granzyme B-mediated processing of IL-1alpha potently enhances the biological activity
-
?
interleukin proIL-18 + H2O
interleukin IL-18 + ?
-
-
cleavage at residues D35-Y36 of proIL-18, identical to cleavage site of caspase-1
-
?
Ki-67 + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: proliferation, associated disease: myositis
-
-
?
Ku-70 + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: DNA repair, associated disease: myositis
-
-
?
La protein + H2O
?
-
La is cleaved by gzmB and gzmH. La is a direct target of gzmH during cytotoxic-mediated cell death. Cleavage of La by gzmH occurs at Phe-364 (P(1) site) and generates a COOH-terminal truncated form of La that loses nuclear localization and decreases hepatitis C virus-internal ribosome entry site-mediated translational activity
-
-
?
Laminin + H2O
?
-
extracellular GrB substrate. Implication: cell adhesion, anoikis
-
-
?
neuronal glutamate receptor + H2O
?
-
extracellular GrB substrate. Implication: GrB cleaves the non-glycosylated form of the receptor into an autoantigenic fragment
-
-
?
nuclear mitotic apparatus protein 1 + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: mitosis, associated disease: Sjörgen's syndrome
-
-
?
nucleolus organizing region 90 kDa (NOR-90/UBF) + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: transcription factor, associated disease: Scleroderma
-
-
?
nucleophosmin (B23) + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: rRNA processing, associated disease: Scleroderma
-
-
?
nucleophosmin + H2O
?
-
efficiently cleaves nucleophosmin of human origin, does not cleave nucleophosmin within J774 cell free extracts
-
-
?
PEG-P + H2O
?
-
virtually all soluble PEG-P is cleaved in 3 min, suggesting PEG-P is a sensitive indicator of granzyme B activity. The granzyme can not access PEG-P when encapsulated in 1,2-dioleoyl-sn-glycero-3-phosphocholine large unilamellar vesicles
-
-
?
plasmin + H2O
?
-
extracellular GrB substrate. Implication: as plasmin is pro-angiogenic, cleavage results in the reduction of angiogenesis
-
-
?
plasminogen + H2O
?
-
extracellular GrB substrate. Implication: cleavage yields angiostatin, which is anti-angiogenic. Implications in angiogenesis
-
-
?
PMScl/EXOSC10 + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: mRNA degradation, associated disease: myositis/Scleroderma
-
-
?
poly(ADP)ribose polymerase 1 (PARP1) + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: ribosylation, associated disease: systemic lupus erythematosus
-
-
?
postmeiotic segregation 1 + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: DNA mismatch repair, associated disease: myositis
-
-
?
postmeiotic segregation 2 + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: DNA mismatch repair, associated disease: myositis
-
-
?
pro-CPP32 + H2O
?
-
processes the caspase into its enzymatically active form
-
?
Protease CMH-1 + H2O
?
-
a close homologue of CPP32, granzyme B specifically cleaves at Asp198-Ser199 between the p20 and p12 and activates the cysteine protease
-
-
?
PTSY-7-amido-4-methylcoumarin + H2O
PTSY + 7-amino-4-methylcoumarin
-
GzmH
-
-
?
pyruvate dehydrogenase complex E2 (PDC-E2) + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: acetyl CoA synthesis, associated disease: primary biliary cirrhosis
-
-
?
RNA polymerase I + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: transcription, associated disease: Scleroderma
-
-
?
RNA polymerase II + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: transcription, associated disease: Scleroderma
-
-
?
signal recognition particle 72 kDa + H2O
?
-
autoantigen cleaved by granzyme B, function of substrate: translation, associated disease: myositis, systemic lupus erythematosus
-
-
?
smooth muscle cell matrix + H2O
?
-
extracellular GrB substrate. Implication: cell adhesion, anoikis
-
-
?