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Literature summary for 3.4.21.79 extracted from

  • D'Angelo, M.E.; Bird, P.I.; Peters, C.; Reinheckel, T.; Trapani, J.A.; Sutton, V.R.
    Cathepsin H is an additional convertase of pro-granzyme B (2010), J. Biol. Chem., 285, 20514-20519.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant human ProGrB is produced in Pichia pastoris Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification GrB is synthesized as a zymogen (proGrB) and activated in cytotoxic granules by cathepsin C and cathepsin H. Mice lacking both cathepsin C and cathepsin H show reduced convertase activity. Despite this, cytotoxic lymphocytes from transgenic mice retain cytotoxic activity and some residual GrB activity, indicating that other proteases must also possess convertase activity Mus musculus
proteolytic modification GrB is synthesized as a zymogen (proGrB) and activated in cytotoxic granules by the lysosomal cysteine protease cathepsin C which removes the N-terminal dipeptide Gly-Glu. Cathepsin H is an additional convertase of proGrB Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
lymphocyte
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-IEPDSSMESK-dinitrophenyl + H2O susbtrate is specific for human GrB Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
granzyme B
-
Mus musculus
granzyme B
-
Homo sapiens
proGrB
-
Mus musculus
proGrB
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Mus musculus
7.4
-
assay at Homo sapiens