Cloned (Comment) | Organism |
---|---|
recombinant human ProGrB is produced in Pichia pastoris | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | GrB is synthesized as a zymogen (proGrB) and activated in cytotoxic granules by cathepsin C and cathepsin H. Mice lacking both cathepsin C and cathepsin H show reduced convertase activity. Despite this, cytotoxic lymphocytes from transgenic mice retain cytotoxic activity and some residual GrB activity, indicating that other proteases must also possess convertase activity | Mus musculus |
proteolytic modification | GrB is synthesized as a zymogen (proGrB) and activated in cytotoxic granules by the lysosomal cysteine protease cathepsin C which removes the N-terminal dipeptide Gly-Glu. Cathepsin H is an additional convertase of proGrB | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lymphocyte | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoyl-IEPDSSMESK-dinitrophenyl + H2O | susbtrate is specific for human GrB | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
granzyme B | - |
Mus musculus |
granzyme B | - |
Homo sapiens |
proGrB | - |
Mus musculus |
proGrB | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
7.4 | - |
assay at | Homo sapiens |