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Literature summary for 3.4.21.79 extracted from

  • Lorentsen, R.H.; Fynbo, C.H.; Thogersen, H.C.; Etzerodt, M.; Holtet, T.L.
    Expression, refolding, and purification of recombinant human granzyme B (2005), Protein Expr. Purif., 39, 18-26.
    View publication on PubMed
Search for more literature for 3.4.21.79

Application

Application Comment Organism
biotechnology engineering of mutant enzyme suitable for cleavage of fusion proteins Homo sapiens

Protein Variants

Protein Variants Comment Organism
C228F fusion protein pro-rGrB-H6, activity with Ac-IEPD-p-nitroanilide substrate as wild-type Homo sapiens
C228T fusion protein pro-rGrB-H6, activity with Ac-IEPD-p-nitroanilide substrate slightly lower than wild-type Homo sapiens
C228V fusion protein pro-rGrB-H6, activity with Ac-IEPD-p-nitroanilide substrate slightly lower than wild-type Homo sapiens
additional information expression of His-tagged enzyme, pro-rGrB-H6, dependent on activation by blood coagulation factor Xa, and of pro(IEPD)-rGrB-H6, engineered for self-activation, and of their C228 mutants Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 IEPD-p-nitroanilide pH 7.75 Homo sapiens
0.067
-
 IEPD-p-nitroanilide pH 7.75 Homo sapiens
0.082
-
 LEED-p-nitroanilide pH 7.75 Homo sapiens
0.088
-
 VEID-p-nitroanilide pH 7.75 Homo sapiens
0.117
-
 LEED-p-nitroanilide pH 7.75 Homo sapiens
0.17
-
 VEID-p-nitroanilide pH 7.75 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27400
-
 x * 27400, calculated for non-activated recombinant fusion protein pro-rGBr-H6 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 recombinant expression
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
IEPD-p-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?
LEED-p-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?
VEID-p-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 27400, calculated for non-activated recombinant fusion protein pro-rGBr-H6 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 LEED-p-nitroanilide pH 7.75 Homo sapiens
0.22
-
 LEED-p-nitroanilide pH 7.75 Homo sapiens
0.3
-
 VEID-p-nitroanilide pH 7.75 Homo sapiens
0.72
-
 VEID-p-nitroanilide pH 7.75 Homo sapiens
4.9
-
 IEPD-p-nitroanilide pH 7.75 Homo sapiens
5
-
 IEPD-p-nitroanilide pH 7.75 Homo sapiens