Application | Comment | Organism |
---|---|---|
analysis | caution in the design and interpretation of experiments using GrBs from different species due to distinct tetrapeptide specificities and abilities to recruit the BID pathway | Mus musculus |
analysis | caution in the design and interpretation of experiments using GrBs from different species due to distinct tetrapeptide specificities and abilities to recruit the BID pathway | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of recombinant GrB using a baculovirus expression system | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-3-((2S,5S)-5-((2S,3S)-2-acetamido-3-methylpentanamido)-4-oxo-1,2,4,5,6,7-hexahydroazepino[3,2,1-hi]indole-2-carboxamido)-4-oxobutanoic acid | peptide-mimetic analog of IEPD-aldehyde | Homo sapiens | |
(S)-3-((2S,5S)-5-((2S,3S)-2-acetamido-3-methylpentanamido)-4-oxo-1,2,4,5,6,7-hexahydroazepino[3,2,1-hi]indole-2-carboxamido)-4-oxobutanoic acid | peptide-mimetic analog of IEPD-aldehyde | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
BID + H2O | Homo sapiens | efficiently cleaves both human or mouse BID | truncated BID + ? | - |
? | |
additional information | Mus musculus | both human or mouse BID are highly resistant to cleavage by mouse GrB | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Mus musculus |
to homogeneity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-IETD-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | acetyl-IETD + 7-amino-4-methylcoumarin | - |
? | |
acetyl-IETD-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | acetyl-IETD + 7-amino-4-methylcoumarin | - |
? | |
BID + H2O | efficiently cleaves both human or mouse BID | Homo sapiens | truncated BID + ? | - |
? | |
additional information | both human or mouse BID are highly resistant to cleavage by mouse GrB | Mus musculus | ? | - |
? | |
additional information | both human or mouse BID are highly resistant to cleavage by mouse GrB. It is unable to cleave BID in cell lysates in which caspases are inactivated but induces BID cleavage, albeit inefficiently, in lysates supporting caspase activity. BID pathway is not a prominent primary mediator of the effects of mouse GrB | Mus musculus | ? | - |
? | |
pro-caspase-3 + H2O | cleaves human pro-caspase-3 but only generates a 21-kDa fragment, no autocatalytic removal of the caspase-3 prodomain. Efficiently cleaves and activates mouse procaspase-3, producing predominantly the 17-kDa fragment | Mus musculus | caspase-3 + ? | - |
? | |
pro-caspase-3 + H2O | cleaves human pro-caspase-3 generating a 21-kDa and a 17-kDa fragment (autocatalytic removal of the caspase-3 prodomain) | Homo sapiens | caspase-3 + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GrB | - |
Mus musculus |
GrB | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000008 | - |
(S)-3-((2S,5S)-5-((2S,3S)-2-acetamido-3-methylpentanamido)-4-oxo-1,2,4,5,6,7-hexahydroazepino[3,2,1-hi]indole-2-carboxamido)-4-oxobutanoic acid | - |
Homo sapiens | |
0.000025 | - |
(S)-3-((2S,5S)-5-((2S,3S)-2-acetamido-3-methylpentanamido)-4-oxo-1,2,4,5,6,7-hexahydroazepino[3,2,1-hi]indole-2-carboxamido)-4-oxobutanoic acid | - |
Mus musculus |