Literature summary for 3.4.21.79 extracted from

Mouchacca, P.; Chasson, L.; Frick, M.; Foray, C.; Schmitt-Verhulst, A.; Boyer, C.
Visualization of granzyme B-expressing CD8 T cells during primary and secondary immune responses to Listeria monocytogenes (2015), Immunology, 145, 24-33 .

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Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of functional fluorescent chimeric GZMB-Tom mutant enzyme fusion protein in murine CD8+ T-cells	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of functional fluorescent chimeric GZMB-Tomato (GZMB-Tom) fusion protein. The GZMB-Tom knock-in mice in which GZMB-Tom faithfully reproduce GZMB expression, provide useful tools to dissect mechanisms leading to the development of anti-bacterial effector and memory CD8+ T-cells and reactivation of the memory response to cognate antigen or inflammatory signals. The use of CD8+ T-cells from knock in mice expressing a functional fluorescent chimeric GZMB-Tom protein in place of GZMB allow the evaluation of the induction of GZMB during CD8+ T-cell differentiation as a function of T-cell division. Mice homozygous for GZMB-Tom and heterozygous for the OT1 (GZMB-Tom-OT1) or OT2 (GZMB-Tom-OT2) transgenic T-cell receptors are used. Congenic C57BL/6 (CD45.1) mice are used as recipients	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	P04187	OT1 and OT2 mice	-
Mus musculus C57BL/6	P04187	OT1 and OT2 mice	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
T-lymphocyte	CD8+ T-cell	Mus musculus	-

Synonyms

Synonyms	Comment	Organism
GzmB	-	Mus musculus

General Information

General Information	Comment	Organism
physiological function	both CD4+ and CD8+ T-cells respond to Listeria monocytogenes, but unlike poorly responding CD4+ T-cells, CD8+ T-cells readily proliferate and express high levels of recombinant chimeric GZMB-Tom as early as 2 days after infection. Using the GZMBTom-OT1 T cells, rapid CD8+ T-cell division and expression of GZMB-Tom following intravenous infection with ActA-/OVA bacteria	Mus musculus

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Release 2023.1 (January 2023)