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Literature summary for 3.4.21.79 extracted from

  • Fellows, E.; Gil-Parrado, S.; Jenne, D.E.; Kurschus, F.C.
    Natural killer cell-derived human granzyme H induces an alternative, caspase-independent cell-death program (2007), Blood, 110, 544-552.
    View publication on PubMed
Search for more literature for 3.4.21.79

Application

Application Comment Organism
medicine GzmH induces cell death, it is nearly as potent as GzmB. Exhibits an alternative cell death pathway in innate immunity. In contrast to GzmB, GzmH achieves cell death by acting on mitochondrial and nuclear targets but not through the activation of hallmark apoptotic substrates Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant, nonglycosylated GzmH and GzmH mutant expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
S195A GzmH mutant, catalytically inactive Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information zVAD-fmk completely inhibits GzmB induced executioner caspase activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information neither monocytes nor activated CD8+ CTLs show any GzmH expression Homo sapiens
-
natural killer cell only the YT cell line, which is a poor killer expresses low levels of GzmH Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-DEVD-7-amido-4-methylcoumarin + H2O efficient cleavage by GzmB after K562 cells are exposed to sublytic perforin or streptolysin O. GzmH shows only baseline readings Homo sapiens ?
-
 ?
acetyl-VDVADAFC + H2O GzmH Homo sapiens acetyl-VDVAD + Ala-Phe-Lys
-
 ?
ATSY-7-amido-4-methylcoumarin + H2O GzmH Homo sapiens ATSY + 7-amino-4-methylcoumarin
-
 ?
additional information caspases 2, 3 and 7 (C285A mutants) and purified BID are not directly cleaved by GzmH. No activity of GzmH toward Ac-LEHD-7-amino-4-methylcouramin Homo sapiens ?
-
 ?
PTSY-7-amido-4-methylcoumarin + H2O GzmH Homo sapiens PTSY + 7-amino-4-methylcoumarin
-
 ?
succinyl-Phe-Leu-Phe-SBzl + H2O GzmH Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
Granzyme H
-
 Homo sapiens
GzmB
-
 Homo sapiens
GzmH
-
 Homo sapiens