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Literature summary for 3.4.21.79 extracted from

  • Cullen, S.P.; Adrain, C.; Luethi, A.U.; Duriez, P.J.; Martin, S.J.
    Human and murine granzyme B exhibit divergent substrate preferences (2007), J. Cell Biol., 176, 435-444.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.79

Application

Application Comment Organism
degradation human and murine GzmB are distinct enzymes with different substrate preferences. Subtle differences in enzyme structure can radically affect substrate selection. Caspases are essential for apoptosis initiated by mouse GzmB Mus musculus
degradation human and murine GzmB are distinct enzymes with different substrate preferences. Subtle differences in enzyme structure can radically affect substrate selection. Caspases are not essential for apoptosis initiated by human GzmB Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Homo sapiens
into vector pPIC6alpha and expressed in Pichia pastoris X-33 cells Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
additional information inhibition of caspases does not clonogenically rescue cells from human GzmB Homo sapiens
additional information inhibition of caspases may clonogenically rescue cells from mouse GzmB Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by nickel affinity chromatography Mus musculus
by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-IETD-7-amido-4-methylcoumarin + H2O
-
 Mus musculus acetyl-IETD + 7-amino-4-methylcoumarin
-
 ?
acetyl-IETD-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens acetyl-IETD + 7-amino-4-methylcoumarin
-
 ?
BID + H2O both human and mouse BID are cleaved efficiently Homo sapiens truncated BID + ?
-
 ?
caspase-8 + H2O cleaves human caspase-8 but fails to cleave the mouse counterpart of caspase-8. Mouse caspase-8 is processed only upon addition of cytochrome c and dATP to J774 extracts to activate the apoptosome pathway to caspase activation Homo sapiens ?
-
 ?
ICAD + H2O cleaves human ICAD but fails to cleave the mouse counterpart Homo sapiens ?
-
 ?
additional information BID is a poor substrate for mouse GzmB. Fails to cleave caspase-8 and ICAD from either human or murine origin. Cleaves nucleophosmin of human origin very inefficiently, does not cleave nucleophosmin within J774 cell free extracts Mus musculus ?
-
 ?
nucleophosmin + H2O efficiently cleaves nucleophosmin of human origin, does not cleave nucleophosmin within J774 cell free extracts Homo sapiens ?
-
 ?
pro-caspase-3 + H2O activates caspase-3, whether of human or murine origin, with a similar efficiency Mus musculus ?
-
 ?
pro-caspase-3 + H2O activates caspase-3, whether of human or murine origin, with a similar efficiency Homo sapiens ?
-
 ?
pro-caspase-7 + H2O activates caspase-7, whether of human or murine origin, with a similar efficiency Mus musculus ?
-
 ?
pro-caspase-7 + H2O activates caspase-7, whether of human or murine origin, with a similar efficiency Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
GzmB
-
 Mus musculus
GzmB
-
 Homo sapiens