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Literature summary for 3.4.21.79 extracted from
- Identification and characterization of equine granzyme B (2007), Vet. Immunol. Immunopathol., 118, 239-251.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ionomycin | stimulates | Equus caballus |  |
| PMA | stimulates | Equus caballus | |
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | Equine granzyme B shows close proximity to putative equine mast cell protease and to granzyme B from mouse, rat, and human. Equine granzyme B may be useful in the development of immunological assays for the activity of equine lymphocytes | Equus caballus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 293TT cells transiently transfected with plasmids encoding either for wild-type or mutant GrzmB. cDNA subcloned into the mammalian expression vector pcDNA3.1(+) or into the insect cell expression vector pVL1392. Expressed in H5 and Sf9 insect cells. Mutagenesis PCR-products transformed into Escherichia coli TOP 10 | Equus caballus |
| cDNA subcloned from pPIC-9-GrzmB yeast-expression vector into pcDNA3.1(+) | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D108A | complete loss of enzyme activity | Equus caballus |
| H64A | complete loss of enzyme activity | Equus caballus |
| additional information | deletion of the activation di-peptide leads to a strong increase in enzyme activity | Equus caballus |
| S203A | complete loss of enzyme activity | Equus caballus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ac-IETD-CHO | reduces activity about 6fold | Equus caballus | |
| IETD-CHO | reduces activity about 6fold | Equus caballus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1512 | - |
Ac-IEPD-p-nitroanilide | - |
Equus caballus | |
| 0.447 | - |
Ac-IEPD-p-nitroanilide | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
Western blot analysis | Equus caballus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Equus caballus | Q2A6L4 | - |
- |
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| peripheral blood mononuclear cell | - |
Equus caballus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ac-IEPD-p-nitroanilide + H2O | - |
Homo sapiens | Ac-IEPD + p-nitroaniline | - |
? | |
| Ac-IEPD-p-nitroanilide + H2O | - |
Equus caballus | Ac-IEPD + p-nitroaniline | - |
? | |
| acetyl-IEPD-p-nitroanilide + H2O | - |
Homo sapiens | ? | - |
? | |
| acetyl-IEPD-p-nitroanilide + H2O | - |
Equus caballus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GrzmB | - |
Homo sapiens |
| GrzmB | - |
Equus caballus |
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Release 2023.1 (January 2023)
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