Information on EC 3.2.1.23 - beta-galactosidase

for references in articles please use BRENDA:EC3.2.1.23
Word Map on EC 3.2.1.23
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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
3.2.1.23
-
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RECOMMENDED NAME
GeneOntology No.
beta-galactosidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
hydrolysis of O-glycosyl bond
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hyrolysis of O-glycosyl bond
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O-glycosyl bond hydrolysis
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
lactose degradation II
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lactose degradation III
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xyloglucan degradation II (exoglucanase)
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metabolism of disaccharids
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Galactose metabolism
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Other glycan degradation
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Glycosaminoglycan degradation
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Sphingolipid metabolism
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Glycosphingolipid biosynthesis - ganglio series
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
beta-D-galactoside galactohydrolase
Some enzymes in this group hydrolyse alpha-L-arabinosides; some animal enzymes also hydrolyse beta-D-fucosides and beta-D-glucosides; cf. EC 3.2.1.108 lactase.
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CAS REGISTRY NUMBER
COMMENTARY hide
9031-11-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
and strain 11-1O
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-
Manually annotated by BRENDA team
cold-active beta-galactosidase, gene bglA
Q08KN3
SwissProt
Manually annotated by BRENDA team
gene bgaS encoding a cold-active enzyme
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-
Manually annotated by BRENDA team
strain ATCC6276
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Manually annotated by BRENDA team
strain ATCC6276
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Manually annotated by BRENDA team
Aspergillus cellulosae
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
L. cv. B10
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-
Manually annotated by BRENDA team
Bacillus sp. (in: Bacteria) No. C-125
No. C-125
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
obligatory anaerobic, thermophilic, Gram-gegative strain NA10
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Manually annotated by BRENDA team
Bacteroides polypragmatus
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-
Manually annotated by BRENDA team
ssp. lactis, strain Bb12
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-
Manually annotated by BRENDA team
Bispora sp.
-
UniProt
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
Caldariella acidophila
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-
-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
Coffea sp.
coffee
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-
Manually annotated by BRENDA team
Corynebacterium murisepticum
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-
Manually annotated by BRENDA team
cultivar Palava
-
-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
strain ATCC8005
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
KCTC 7534
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
i.e. Polyporus tulipiferae
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Manually annotated by BRENDA team
Kerstingiella geocarpa
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-
Manually annotated by BRENDA team
Kestingiella geocarpa
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-
Manually annotated by BRENDA team
and strain A60
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-
Manually annotated by BRENDA team
strain DSM5418
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
three strains, isolated form human faeces
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Manually annotated by BRENDA team
ssp. lactis, two strains, isolated form human faeces
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Manually annotated by BRENDA team
two strains, isolated form human faeces
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Manually annotated by BRENDA team
two strains, isolated form human faeces
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-
Manually annotated by BRENDA team
several strains, isolated form human faeces
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-
Manually annotated by BRENDA team
CNRZ 131
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Manually annotated by BRENDA team
three strains, isolated form human faeces
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Manually annotated by BRENDA team
two strains, isolated form human faeces
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-
Manually annotated by BRENDA team
isolated form human faeces
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-
Manually annotated by BRENDA team
two strains, isolated form human faeces
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Manually annotated by BRENDA team
isolated form human faeces
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Manually annotated by BRENDA team
isolated form human faeces
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
Leuconostoc citrovorum
-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
-
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-
Manually annotated by BRENDA team
-
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-
Manually annotated by BRENDA team
-
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-
Manually annotated by BRENDA team
no activity in Lactobacillus gasseri
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-
Manually annotated by BRENDA team
no activity in Phaeobacter gallaeciensis BS107
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-
Manually annotated by BRENDA team
no activity in Thalassobius aestuarii
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-
Manually annotated by BRENDA team
no activity in Thalassobius aestuarii JC2049
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-
Manually annotated by BRENDA team
no activity in Thalassobius mediterraneus
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Manually annotated by BRENDA team
no activity in Thalassobius mediterraneus XSM19
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Paracolobactrum aerogenoides
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Manually annotated by BRENDA team
Penicillium glaucum
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Penicillium toxidarium
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
cv. PDR14
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
almond
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
TAE 79
SwissProt
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Rhizopus acidus
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Manually annotated by BRENDA team
Rhizopus formosciensis
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Saccharomyces anamensis
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Manually annotated by BRENDA team
Saccharomyces fragilis
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Manually annotated by BRENDA team
Saccharomyces fragilis KY5463
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Manually annotated by BRENDA team
Saccharomyces lactis
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Sporotrichum sp.
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
serotype 2, isolate JX081101
C5VV31
UniProt
Manually annotated by BRENDA team
Sulfolobus solfataricus MT-4 / DSM 5833
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
IAM 12617
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Manually annotated by BRENDA team
UST050418-052
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Manually annotated by BRENDA team
Thermoanaerobium sp.
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Torulopsis sphaerica
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Manually annotated by BRENDA team
Torulopsis sphaerica J28
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Manually annotated by BRENDA team
Torulopsis versatilis
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Manually annotated by BRENDA team
Torulopsis versatilis M6
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Manually annotated by BRENDA team
Reiter strain
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Manually annotated by BRENDA team
Reiter strain
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Manually annotated by BRENDA team
two isozymes BGT I and BGT II
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
L. cv. Egret
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
a metagenomic library from topsoil of Daqing oil field of Heilongjiang Province in China is constructed for the screening of clones with beta-galactosidase activity
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Zygosaccharomyces lactis
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-
Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2R)-glycerol-O-D-beta-galactopyranoside + H2O
beta-D-galactose + glycerol
show the reaction diagram
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-
-
-
?
1-naphthyl beta-D-galactopyranoside + H2O
1-naphthol + beta-D-galactopyranose
show the reaction diagram
-
-
-
-
?
1-naphthyl-beta-D-galactoside + H2O
beta-D-galactose + 1-naphthol
show the reaction diagram
1-naphthyl-beta-D-glucoside + H2O
beta-D-glucose + 1-naphthol
show the reaction diagram
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside + H2O
2,4-dinitrophenol + 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside
show the reaction diagram
-
-
-
-
?
2,4-dinitrophenyl-beta-D-galactoside + H2O
2,4-dinitrophenol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
2,4-dinitrophenyl-beta-D-glucoside + H2O
2,4-dinitrophenol + beta-D-glucose
show the reaction diagram
-
activity is 10000 times lower than with 2,4-dinitrophenyl-beta-D-galactoside
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-
?
2-nitrophenyl alpha-D-galactopyranoside + H2O
2-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
1.62% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
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-
?
2-nitrophenyl beta-D-galactopyranose + H2O
2-nitrophenol + D-galactose
show the reaction diagram
2-nitrophenyl beta-D-galactopyranoside + D-mannitol
beta-D-galactopyranosyl-(1-1)-D-mannitol + 2-nitrophenol
show the reaction diagram
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
show the reaction diagram
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
show the reaction diagram
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactopyranose
show the reaction diagram
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
show the reaction diagram
2-nitrophenyl beta-D-galactopyranoside + L-sorbose
beta-D-galactopyranosyl-(1-1)-L-sorbose + 2-nitrophenol
show the reaction diagram
2-nitrophenyl beta-D-galactopyranoside + salicin
2-(hydroxymethyl)phenyl beta-D-galactopyranosyl-(1->6)-beta-D-glucopyranoside + 2-nitrophenol
show the reaction diagram
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + beta-D-galactose
show the reaction diagram
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + D-galactose
show the reaction diagram
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranose
show the reaction diagram
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranoside
show the reaction diagram
-
-
-
-
?
2-nitrophenyl-alpha-D-mannopyranoside + H2O
2-nitrophenol + alpha-D-mannopyranose
show the reaction diagram
-
0.31% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
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-
?
2-nitrophenyl-beta-D-fucopyranoside + H2O
2-nitrophenol + beta-D-fucose
show the reaction diagram
-
-
-
?
2-phenylethyl-beta-D-galactoside + H2O
2-phenylethanol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
3'-deoxy-5-fluorouridine + beta-D-galactose
3'-deoxy-5'-O-beta-D-galactosyl-5-fluorouridine
show the reaction diagram
-
5-O-beta-D-galactosylfloxuridine is synthesized with a yield of 75% through beta-galactosidase-catalyzed transgalactosylation. Enzyme displays absolute regioselectivity toward the 5'-position of floxuridine. Reaction condition: pH 6.5, 45°C for 60 h
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-
3,4-dinitrophenyl beta-D-galactopyranoside + H2O
3,4-dinitrophenol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
3-nitrophenyl beta-D-galactopyranoside + H2O
3-nitrophenol + beta-D-galactopyranose
show the reaction diagram
-
0.13% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-methylumbelliferyl beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
show the reaction diagram
4-methylumbelliferyl-alpha-L-D-arabinoside + H2O
4-methylumbelliferone + alpha-L-arabinose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-galactopyranoside + H2O
4-methylumbelliferone + beta-D-galactopyranose
show the reaction diagram
-
pH 4.0, 30 min, 37°C
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-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
show the reaction diagram
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
100% activity
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-
?
4-nitrophenyl alpha-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
show the reaction diagram
42% activity compared to 4-nitrophenyl alpha-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-arabinoside + H2O
4-nitrophenol + beta-D-arabinose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
show the reaction diagram
3% activity compared to 4-nitrophenyl alpha-D-galactopyranoside
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-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucose
show the reaction diagram
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
show the reaction diagram
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
show the reaction diagram
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
show the reaction diagram
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + beta-D-galactose
show the reaction diagram
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + D-galactose
show the reaction diagram
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
-
low activity
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
show the reaction diagram
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronic acid
show the reaction diagram
-
0.69% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannopyranose
show the reaction diagram
-
0.9% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-mannoside + H2O
4-nitrophenol + beta-D-mannose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
show the reaction diagram
-
0.31% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose
show the reaction diagram
-
1.18% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinose
show the reaction diagram
-
-
-
?
4-nitrophenyl-alpha-L-arabinoside + H2O
4-nitrophenol + alpha-L-arabinose
show the reaction diagram
-
0.74% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl-beta-L-arabinoside + H2O
4-nitrophenol + beta-L-arabinose
show the reaction diagram
-
0.21% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside + H2O
5-bromo-4-chloro-indol + beta-D-galactose
show the reaction diagram
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloro-indol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + beta-D-galactopyranose
show the reaction diagram
-
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + beta-D-galactose
show the reaction diagram
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + D-galactose
show the reaction diagram
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
?
show the reaction diagram
5-bromo-4-chloro-3-indolyl-beta-D-galactoside + H2O
5-bromo-4-chloroindol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
5-bromo-4-chloro-3-indoxyl-beta-galactoside + H2O
5-bromo-4-chloro-3-indoxol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
5-dodecanoylaminofluorescein di-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
6-bromo-2-naphthyl-beta-galactoside + H2O
6-bromo-2-naphthol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
6-bromo-2-naphthyl-beta-galactoside + H2O
6-bromo-2-naphthol + D-galactose
show the reaction diagram
7-O-beta-D-galactosylresorufin + H2O
resorufin + beta-D-galactose
show the reaction diagram
-
-
-
-
?
9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) beta-D-galactopyranoside + H2O
9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-ol) + beta-D-galactose
show the reaction diagram
-
-
-
-
?
9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl)-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl)-beta-D-galactoside + H2O
?
show the reaction diagram
-
assays are performed on individual Escherichia coli beta-galactosidase molecules at 2 different concentrations of the substrate DDAO-beta-D-galactoside using a free zone capillary electrophoresis-based protocol with an in-laboratory-constructed instrument utilizing laser-induced fluorescence detection
-
-
?
a beta-D-galactoside + H2O
D-galactose + an alcohol
show the reaction diagram
agarose + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
-
?
allolactose + H2O
D-galactose + D-glucose
show the reaction diagram
asialo alpha1-acid glycoprotein + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
?
asialofetuin + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
?
beta-(1->3)-D-galactan + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
-
?
beta-1,3-galactobiose + H2O
beta-D-galactose
show the reaction diagram
beta-1,3-galactotriitol + H2O
?
show the reaction diagram
-
-
-
-
?
beta-1,3-galactotriose + H2O
?
show the reaction diagram
beta-1,6-galactobiose + H2O
beta-D-galactose
show the reaction diagram
-
-
-
-
?
beta-1,6-galactotriitol + H2O
?
show the reaction diagram
-
-
-
-
?
beta-1,6-galactotriose + H2O
?
show the reaction diagram
-
-
-
-
?
beta-D-(1-6) galactobiose + H2O
?
show the reaction diagram
beta-D-galactosyl azide + H2O
azide + beta-D-galactose
show the reaction diagram
-
-
-
-
?
beta-D-galactosyl fluoride + H2O
fluoride + beta-D-galactose
show the reaction diagram
-
-
-
-
?
butyl O-beta-galactoside + H2O
butanol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
cellobiose + H2O
2 D-glucose
show the reaction diagram
-
41.7% of the activtiy with lactose
-
-
?
cellobiose + H2O
D-galactose
show the reaction diagram
ceramide lacto-N-tetraose + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
?
chlorophenol red beta-D-galactopyranoside + H2O
chlorophenol red + beta-D-galactose
show the reaction diagram
-
-
-
-
?
digalactosyl ceramide + H2O
?
show the reaction diagram
-
-
-
-
?
ethyl-beta-D-galactoside + H2O
ethanol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
exopolysaccharide + H2O
beta-D-galactose + ?
show the reaction diagram
-
modified exopolysaccharides produced by Lactococcus lactis subsp. cremonis B39 and B891
-
-
?
fluorescein-beta-D-digalactopyranoside + H2O
fluorescein + beta-D-galactose
show the reaction diagram
-
-
-
-
?
Gal-alpha(1,3)-Gal-beta-2,4-dinitrophenyl + H2O
?
show the reaction diagram
-
-
-
-
?
galactan + H2O
?
show the reaction diagram
-
the GHF 42 enzyme LacA can act on the oligosaccharides released by the GH53 enzyme GalA from galactan, overview
-
-
?
galactan + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
-
?
galactan + H2O
galactose
show the reaction diagram
-
-
+ small amounts of glucose and arabinose
-
galactobiose + H2O
?
show the reaction diagram
-
-
-
?
galactosyl-fetuin + H2O
galactose + fetuin
show the reaction diagram
-
-
-
-
?
galactosyl-ovomucoid + H2O
galactose + ovomucoid
show the reaction diagram
-
-
-
-
?
galactotetraose + H2O
beta-D-galactose
show the reaction diagram
-
the GHF 42 enzyme LacA can act on the oligosaccharides released by the GH53 enzyme GalA from galactan, overview
-
-
?
galactoxyloglucan + H2O
galactose + ?
show the reaction diagram
-
-
-
-
?
gentiobiose + H2O
2 beta-D-glucose
show the reaction diagram
-
reaction of EC 3.2.1.21, 14.3% of the activtiy with lactose
-
-
?
gentiobiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
14.3% of the activity with lactose
-
-
?
glycoasparagines + H2O
galactose + ?
show the reaction diagram
-
hydrolysis of beta-galactosyl linkages in urinary glycoasparagines
-
?
glycolipid GM1 ganglioside + H2O
beta-D-galactose + ?
show the reaction diagram
keratan sulfate + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
?
lactitol + H2O
D-galactose + D-glucitol
show the reaction diagram
lactose + 2-phenylethanol
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + alpha-D-methyl-galactoside
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + alpha-D-methyl-glucoside
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + arabinose
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + benzyloxycarbonyl-Hyp-methyl ester
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + benzyloxycarbonyl-Ser-methyl ester
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + benzyloxycarbonyl-Thr-methyl ester
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + cellobiose
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + fructose
?
show the reaction diagram
lactose + glucose
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + H2O
beta-D-glucose + beta-D-galactose
show the reaction diagram
lactose + H2O
D-galactose + D-glucose
show the reaction diagram
lactose + H2O
D-galactose + D-glucose + oligosaccharide
show the reaction diagram
Bispora sp.
under simulated gastric conditions, BgalA has greater stability (about 100%) and hydrolysis ratio (more than 80%) toward milk lactose than the commercially available beta-galactosidase from Aspergillus oryzae (ATCC 20423)
the main products of lactose hydrolysis by recombinant BgalA are 19.5% D-galactose, 72.5% D-glucose, and 8.0% oligosaccharide
-
?
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
lactose + H2O
galactooligosaccharides
show the reaction diagram
lactose + hydroquinone
hydroquinone galactoside + D-glucose
show the reaction diagram
lactose + lactulose
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + lyxose
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + myo-inositol
6-beta-O-D-galactopyranosyl myo-inositol + ?
show the reaction diagram
-
-
-
?
lactose + p-nitrophenyl beta-D-glucoside
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + rhamnose
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + sucrose
?
show the reaction diagram
-
transgalactosylation
-
-
?
lactose + xylose
?
show the reaction diagram
lactosucrose + H2O
D-galactose + ?
show the reaction diagram
lactosyl ceramide + H2O
?
show the reaction diagram
lactulose + H2O
?
show the reaction diagram
lactulose + H2O
D-fructose + D-galactose
show the reaction diagram
lactulose + H2O
D-galactose + D-fructose
show the reaction diagram
methyl beta-D-galactohexaoside + H2O
?
show the reaction diagram
methyl beta-D-galactopentaoside + H2O
?
show the reaction diagram
methyl beta-D-galactotetraoside + H2O
?
show the reaction diagram
methyl O-beta-galactoside + H2O
methanol + beta-D-galactose
show the reaction diagram
methyl salicylate beta-D-galactoside + H2O
methyl salicylate + beta-D-galactose
show the reaction diagram
-
-
-
-
?
methyl-beta-D-galactopyranoside + H2O
methanol + beta-D-galactose
show the reaction diagram
methylthio-beta-D-galactoside + H2O
galactose + methanethiol
show the reaction diagram
monogalactosyldiacylglycerol + H2O
galactose + diacylglycerol
show the reaction diagram
-
-
-
?
monosialoganglioside + H2O
?
show the reaction diagram
-
-
-
-
?
O-beta-D-galactosyl-1,3-D-arabinoside + H2O
beta-D-galactose + D-arabinose
show the reaction diagram
o-nitrophenyl beta-D-fucopyranoside + H2O
o-nitrophenol + beta-D-fucose
show the reaction diagram
6.1% of the activity with o-nitrophenyl beta-D-galactopyranoside
-
-
?
o-nitrophenyl beta-D-fucoside + H2O
o-nitrophenol + beta-D-fucose
show the reaction diagram
o-nitrophenyl beta-D-galactopyranoside + H2O
o-nitrophenol + beta-D-galactose
show the reaction diagram
o-nitrophenyl-beta-D-galactoside + H2O
o-nitrophenol + beta-D-galactose
show the reaction diagram
o-nitrophenyl-beta-D-galactoside + myo-inositol
?
show the reaction diagram
-
transgalactosylation
-
-
?
p-nitrophenyl alpha-arabinofuranoside + H2O
p-nitrophenol + alpha-arabinose
show the reaction diagram
-
weak activity
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-mannopyranoside + H2O
p-nitrophenol + alpha-D-mannose
show the reaction diagram
-
beta-galactosidase I: 0.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 49.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 0.5% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl alpha-L-arabinopyranoside + H2O
p-nitrophenol + alpha-L-arabinopyranose
show the reaction diagram
-
beta-galactosidase I: 20.1% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 32.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 24.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 12.5% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 18.5% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl alpha-L-arabinoside + H2O
p-nitrophenol + alpha-L-arabinose
show the reaction diagram
p-nitrophenyl alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucose
show the reaction diagram
-
beta-galactosidase I: 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 1.1% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 1.2% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 4.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 1.6% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl alpha-L-galactopyranoside + H2O
p-nitrophenol + alpha-L-galactose
show the reaction diagram
-
beta-galactosidase I: 2.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 3.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 3.9% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl beta-D-fucopyranoside + H2O
p-nitrophenol + beta-D-fucose
show the reaction diagram
p-nitrophenyl beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl beta-D-galactopyranoside + H2O
p-nitrophenol + beta-D-galactose
show the reaction diagram
p-nitrophenyl beta-D-glucopyranoside + H2O
p-nitrophenol + beta-D-glucose
show the reaction diagram
p-nitrophenyl beta-D-glucuronide + H2O
p-nitrophenol + ?
show the reaction diagram
-
beta-galactosidase I: 4.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 3.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 15.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 64% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannose
show the reaction diagram
-
beta-galactosidase I: 0.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 0.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 4.2% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 0.5% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylose
show the reaction diagram
p-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
p-nitrophenol + N-acetyl-beta-D-glucosamine
show the reaction diagram
-
beta-galactosidase I: 2.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 4.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 10.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl-beta-D-galactopyranoside + H2O
p-nitrophenol + beta-D-galactose
show the reaction diagram
p-nitrophenyl-beta-D-galactoside + alpha-D-methyl-galactoside
?
show the reaction diagram
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + alpha-D-methyl-glucoside
?
show the reaction diagram
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + D-glucose
lactose + p-nitrophenol
show the reaction diagram
-
transgalactosylation
-
?
p-nitrophenyl-beta-D-galactoside + glucose
?
show the reaction diagram
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + beta-D-galactose
show the reaction diagram
p-nitrophenyl-beta-D-galactoside + myo-inositol
?
show the reaction diagram
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + p-nitrophenyl beta-D-glucoside
?
show the reaction diagram
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + sucrose
?
show the reaction diagram
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + xylose
?
show the reaction diagram
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
show the reaction diagram
p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + beta-D-glucose
show the reaction diagram
-
60% of the activity with p-nitrophenyl-beta-D-galactoside
-
-
?
pectic galactan + H2O
?
show the reaction diagram
-
-
-
-
?
pectic galactan + H2O
galactose + ?
show the reaction diagram
-
-
-
-
?
pectic polysaccharides + H2O
?
show the reaction diagram
-
1.27% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
phenyl-alpha-D-galactoside + H2O
phenol + alpha-D-galactose
show the reaction diagram
-
14% of the activity with o-nitrophenyl-beta-D-galactoside
-
-
?
phenyl-beta-D-galactopyranoside + H2O
phenol + beta-D-galactose
show the reaction diagram
phenyl-beta-D-galactoside + H2O
phenol + beta-D-galactose
show the reaction diagram
phlorizin + H2O
D-galactose + ?
show the reaction diagram
-
-
-
-
?
resorufin-beta-D-galactoside + H2O
?
show the reaction diagram
-
-
-
-
?
tetrahexosyl ceramide + H2O
?
show the reaction diagram
-
-
-
-
?
thio-(o-nitrophenyl)-beta-D-galactoside + H2O
thio-(o-nitrophenol) + beta-D-galactose
show the reaction diagram
-
-
-
-
?
trihexosyl ceramide + H2O
?
show the reaction diagram
-
-
-
-
?
xyloglucan + H2O
?
show the reaction diagram
-
structural xyloglucans show 0.57% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside, reserve xyloglucans show 1.8% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
xyloglucan oligosaccharides + H2O
?
show the reaction diagram
lactose
additional information
-