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Information on EC 3.2.1.23 - beta-galactosidase
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3.2.1.23 beta-galactosidaseIUBMB Comments
Some enzymes in this group hydrolyse alpha-L-arabinosides; some animal enzymes also hydrolyse beta-D-fucosides and beta-D-glucosides; cf. EC 3.2.1.108 lactase.
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Word Map
- 3.2.1.23
- lacz
- adenovirus
- lysosomal
- lactose
- endothelial
- artery
- oligosaccharide
- beta-glucuronidase
- lambda
- beta-glucosidase
-
adenovirus-mediated
- luciferase
-
retroviral
-
immunoassay
- simplex
-
herpes
- phage
- hydrolases
- liposome
- glycosidases
- cytomegalovirus
- neuraminidase
- bacteriophage
- viruses
- gm1
- telomerase
-
replication-defective
-
sialic
-
adeno-associated
-
galactosylation
- n-acetyl-beta-glucosaminidase
- alpha-glucosidase
- alpha-mannosidase
- ganglioside
- vaccinia
- alpha-galactosidase
- sialidase
-
naked
- kluyveromyces
-
plaque-forming
- biotechnology
- molecular biology
- industry
- hexosaminidase
- degradation
- diagnostics
- synthesis
-
lysogens
- keratan
- environmental protection
- biofuel production
-
nonviral
-
vector-mediated
- food industry
- ganciclovir
- medicine
- analysis
- p16ink4a
- nutrition
-
transglycosylation
- freundii
- beta-hexosaminidase
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
beta-galactosidase, beta-gal, beta-d-galactosidase, senescence-associated beta-galactosidase, endo-beta-galactosidase, bglap, sa-beta-gal, acid beta-galactosidase, beta galactosidase, betagal, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Acid beta-galactosidase
BbgI
BbgII
BbgIII
BbgIV
beta-D-galactohydrolase
beta-D-galactoside galactohydrolase
beta-gal
beta-Gal 1
beta-Gal 2
beta-galactosidase
betagal
BGA
BgaB
BgaBM
bgaD
BgaH
BgaS
BGL1
BglAp
BGT I
BGT II
cold-active beta-galactosidase
GALA
galactosidase
Hlac_2868
LacA
LACS
lactase
MeBglD2
beta-glucosidase/galactosidase/fucosidase activity, cf. 3.2.1.21 and 3.2.1.38
ONPGase
senescence-associated beta-galactosidase
Ss beta-gal
SSO3019
XC1214
additional information
additional information
the enzyme belongs to the glycosyl hydrolase family 42
additional information
the enzyme belongs to the glycosyl hydrolase family 2
additional information
the enzyme belongs to the glycosyl hydrolase family 2
additional information
-
the enzymes LacAand YesZ, and GalA belong to the glycosyl hydrolase families 42 and 43, respectively
additional information
-
the enzyme belongs to the glycosyl hydrolase family 35
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
mechanism
-
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
catalytic mechanism, active site structure,Glu157 acts as the acid/base in the catalytic reaction
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Glu184 acts as acid/base catalyst
-
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
the conserved residues E482, M522, Y523 and E551 are important in catalysis
-
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
mechanism
-
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
mechanism
-
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
mechanism
-
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
the conserved residues E482, M522, Y523 and E551 are important in catalysis
-
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
mechanism
-
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis
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PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
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SYSTEMATIC NAME
IUBMB Comments
beta-D-galactoside galactohydrolase
Some enzymes in this group hydrolyse alpha-L-arabinosides; some animal enzymes also hydrolyse beta-D-fucosides and beta-D-glucosides; cf. EC 3.2.1.108 lactase.
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CAS REGISTRY NUMBER
COMMENTARY
9031-11-2
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2R)-glycerol-O-D-beta-galactopyranoside + H2O
beta-D-galactose + glycerol
-
-
-
?
1-naphthyl beta-D-galactopyranoside + H2O
1-naphthol + beta-D-galactopyranose
-
-
-
?
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside + H2O
2,4-dinitrophenol + 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside
-
-
-
?
2,4-dinitrophenyl-beta-D-galactoside + H2O
2,4-dinitrophenol + beta-D-galactose
-
-
-
?
2,4-dinitrophenyl-beta-D-glucoside + H2O
2,4-dinitrophenol + beta-D-glucose
-
activity is 10000 times lower than with 2,4-dinitrophenyl-beta-D-galactoside
-
?
2-nitrophenyl alpha-D-galactopyranoside + H2O
2-nitrophenol + alpha-D-galactopyranose
-
1.62% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
?
2-nitrophenyl beta-D-galactopyranoside + D-mannitol
beta-D-galactopyranosyl-(1-1)-D-mannitol + 2-nitrophenol
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactopyranose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + L-sorbose
beta-D-galactopyranosyl-(1-1)-L-sorbose + 2-nitrophenol
2-nitrophenyl beta-D-galactopyranoside + salicin
2-(hydroxymethyl)phenyl beta-D-galactopyranosyl-(1->6)-beta-D-glucopyranoside + 2-nitrophenol
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranoside
-
-
-
?
2-nitrophenyl-alpha-D-mannopyranoside + H2O
2-nitrophenol + alpha-D-mannopyranose
-
0.31% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
?
2-nitrophenyl-beta-D-fucopyranoside + H2O
2-nitrophenol + beta-D-fucose
-
-
?
2-phenylethyl-beta-D-galactoside + H2O
2-phenylethanol + beta-D-galactose
-
-
-
?
3'-deoxy-5-fluorouridine + beta-D-galactose
3'-deoxy-5'-O-beta-D-galactosyl-5-fluorouridine
-
5-O-beta-D-galactosylfloxuridine is synthesized with a yield of 75% through beta-galactosidase-catalyzed transgalactosylation. Enzyme displays absolute regioselectivity toward the 5'-position of floxuridine. Reaction condition: pH 6.5, 45°C for 60 h
-
?
3,4-dinitrophenyl beta-D-galactopyranoside + H2O
3,4-dinitrophenol + beta-D-galactose
-
-
-
?
3-nitrophenyl beta-D-galactopyranoside + H2O
3-nitrophenol + beta-D-galactopyranose
-
0.13% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
?
4-methylumbelliferyl alpha-L-arabinoside + H2O
4-methylumbelliferone + alpha-L-arabinose
-
-
-
?
4-methylumbelliferyl-beta-D-galactopyranoside + H2O
4-methylumbelliferone + beta-D-galactopyranose
-
pH 4.0, 30 min, 37°C
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
100% activity
-
?
4-nitrophenyl alpha-D-glucopyranoside + H2O
4-nitrophenol + alpha-D-glucopyranose
7.4% of the activity with 4-nitrophenyl beta-D-galactopyranoside
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
42% activity compared to 4-nitrophenyl alpha-D-galactopyranoside
-
?
4-nitrophenyl beta-D-arabinoside + H2O
4-nitrophenol + beta-D-arabinose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
-
low activity
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronic acid
-
0.69% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannopyranose
-
0.9% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-mannoside + H2O
4-nitrophenol + beta-D-mannose
-
-
-
?
4-nitrophenyl beta-L-arabinoside + H2O
4-nitrophenol + beta-L-arabinose
-
0.21% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
?
4-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose
-
1.18% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinose
-
-
?
4-nitrophenyl-alpha-L-arabinoside + H2O
4-nitrophenol + alpha-L-arabinose
-
0.74% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
?
5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside + H2O
5-bromo-4-chloro-indol + beta-D-galactose
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloro-indol + beta-D-galactose
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + beta-D-galactopyranose
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + beta-D-galactose
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + D-galactose
5-bromo-4-chloro-3-indolyl-beta-D-galactoside + H2O
5-bromo-4-chloroindol + beta-D-galactose
-
-
-
?
5-bromo-4-chloro-3-indoxyl-beta-galactoside + H2O
5-bromo-4-chloro-3-indoxol + beta-D-galactose
-
-
-
?
6-bromo-2-naphthyl-beta-galactoside + H2O
6-bromo-2-naphthol + beta-D-galactose
-
-
-
?
9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) beta-D-galactopyranoside + H2O
9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-ol) + beta-D-galactose
-
-
-
?
9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl)-beta-D-galactopyranoside + H2O
?
-
-
-
?
9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl)-beta-D-galactoside + H2O
?
-
assays are performed on individual Escherichia coli beta-galactosidase molecules at 2 different concentrations of the substrate DDAO-beta-D-galactoside using a free zone capillary electrophoresis-based protocol with an in-laboratory-constructed instrument utilizing laser-induced fluorescence detection
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose
-
-
?
beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose
-
-
?
beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose
-
-
?
beta-D-Galp-(1->3)-beta-D-Galp-(1->3)-D-galactitol + H2O
beta-D-Galp-(1->3)-D-galactitol + D-galactopyranose
-
-
-
?
beta-D-Galp-(1->6)-beta-D-Galp-(1->6)-D-galactitol + H2O
beta-D-Galp-(1->6)-D-galactitol + D-galactopyranose
-
-
-
?
cellobiose + H2O
2 D-glucose
-
41.7% of the activtiy with lactose
-
?
chlorophenol red beta-D-galactopyranoside + H2O
chlorophenol red + beta-D-galactose
-
-
-
?
chlorophenol-red beta-D-galactopyranoside + H2O
chlorophenol-red + beta-D-galactopyranose
-
-
-
?
exopolysaccharide + H2O
beta-D-galactose + ?
-
modified exopolysaccharides produced by Lactococcus lactis subsp. cremonis B39 and B891
-
?
fluorescein-beta-D-digalactopyranoside + H2O
fluorescein + beta-D-galactose
-
-
-
?
galactan + H2O
?
-
the GHF 42 enzyme LacA can act on the oligosaccharides released by the GH53 enzyme GalA from galactan, overview
-
?
galactan + H2O
galactose
-
-
+ small amounts of glucose and arabinose
?
galactotetraose + H2O
beta-D-galactose
-
the GHF 42 enzyme LacA can act on the oligosaccharides released by the GH53 enzyme GalA from galactan, overview
-
?
gentiobiose + H2O
2 beta-D-glucose
-
reaction of EC 3.2.1.21, 14.3% of the activtiy with lactose
-
?
gentiobiose + H2O
D-galactose + D-glucose
-
14.3% of the activity with lactose
-
?
glycoasparagines + H2O
galactose + ?
-
hydrolysis of beta-galactosyl linkages in urinary glycoasparagines
-
?
lactose + benzyloxycarbonyl-Hyp-methyl ester
?
-
transgalactosylation
-
?
lactose + benzyloxycarbonyl-Ser-methyl ester
?
-
transgalactosylation
-
?
lactose + benzyloxycarbonyl-Thr-methyl ester
?
-
transgalactosylation
-
?
lactose + H2O
D-galactose + D-glucose + oligosaccharide
under simulated gastric conditions, BgalA has greater stability (about 100%) and hydrolysis ratio (more than 80%) toward milk lactose than the commercially available beta-galactosidase from Aspergillus oryzae (ATCC 20423)
the main products of lactose hydrolysis by recombinant BgalA are 19.5% D-galactose, 72.5% D-glucose, and 8.0% oligosaccharide
?
lactose + myo-inositol
6-beta-O-D-galactopyranosyl myo-inositol + ?
-
-
-
?
lactose + p-nitrophenyl beta-D-glucoside
?
-
transgalactosylation
-
?
methyl salicylate beta-D-galactoside + H2O
methyl salicylate + beta-D-galactose
-
-
-
?
monogalactosyldiacylglycerol + H2O
galactose + diacylglycerol
-
-
-
?
nitrophenyl beta-D-galactoside + H2O
nitrophenol + beta-D-galactose
-
-
-
?
o-nitrophenyl beta-D-fucopyranoside + H2O
o-nitrophenol + beta-D-fucose
6.1% of the activity with o-nitrophenyl beta-D-galactopyranoside
-
?
o-nitrophenyl-beta-D-galactoside + myo-inositol
?
-
transgalactosylation
-
?
p-nitrophenyl alpha-arabinofuranoside + H2O
p-nitrophenol + alpha-arabinose
-
weak activity
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
?
p-nitrophenyl alpha-D-mannopyranoside + H2O
p-nitrophenol + alpha-D-mannose
-
beta-galactosidase I: 0.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 49.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 0.5% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
?
p-nitrophenyl alpha-L-arabinopyranoside + H2O
p-nitrophenol + alpha-L-arabinopyranose
-
beta-galactosidase I: 20.1% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 32.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 24.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 12.5% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 18.5% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
?
p-nitrophenyl alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucose
-
beta-galactosidase I: 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 1.1% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 1.2% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 4.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 1.6% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
?
p-nitrophenyl alpha-L-galactopyranoside + H2O
p-nitrophenol + alpha-L-galactose
-
beta-galactosidase I: 2.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 3.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 3.9% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
?
p-nitrophenyl beta-D-glucuronide + H2O
p-nitrophenol + ?
-
beta-galactosidase I: 4.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 3.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 15.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 64% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
?
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannose
-
beta-galactosidase I: 0.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 0.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 4.2% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 0.5% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
?
p-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
p-nitrophenol + N-acetyl-beta-D-glucosamine
-
beta-galactosidase I: 2.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 4.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 10.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
?
p-nitrophenyl-beta-D-galactopyranoside + H2O
p-nitrophenol + beta-D-galactose
-
-
?
p-nitrophenyl-beta-D-galactoside + alpha-D-methyl-galactoside
?
-
transgalactosylation
-
?
p-nitrophenyl-beta-D-galactoside + alpha-D-methyl-glucoside
?
-
transgalactosylation
-
?
p-nitrophenyl-beta-D-galactoside + D-glucose
lactose + p-nitrophenol
-
transgalactosylation
-
?
p-nitrophenyl-beta-D-galactoside + glucose
?
-
transgalactosylation
-
?
p-nitrophenyl-beta-D-galactoside + myo-inositol
?
-
transgalactosylation
-
?
p-nitrophenyl-beta-D-galactoside + p-nitrophenyl beta-D-glucoside
?
-
transgalactosylation
-
?
p-nitrophenyl-beta-D-galactoside + sucrose
?
-
transgalactosylation
-
?
p-nitrophenyl-beta-D-galactoside + xylose
?
-
transgalactosylation
-
?
p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + beta-D-glucose
-
60% of the activity with p-nitrophenyl-beta-D-galactoside
-
?
pectic polysaccharides + H2O
?
-
1.27% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
?
phenyl-alpha-D-galactoside + H2O
phenol + alpha-D-galactose
-
14% of the activity with o-nitrophenyl-beta-D-galactoside
-
?
thio-(o-nitrophenyl)-beta-D-galactoside + H2O
thio-(o-nitrophenol) + beta-D-galactose
-
-
-
?
xyloglucan + H2O
?
-
structural xyloglucans show 0.57% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside, reserve xyloglucans show 1.8% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
?
1-naphthyl-beta-D-galactoside + H2O
beta-D-galactose + 1-naphthol
-
-
-
?
1-naphthyl-beta-D-galactoside + H2O
beta-D-galactose + 1-naphthol
-
-
-
?
1-naphthyl-beta-D-glucoside + H2O
beta-D-glucose + 1-naphthol
-
-
-
?
2-nitrophenyl beta-D-galactopyranose + H2O
2-nitrophenol + D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranose + H2O
2-nitrophenol + D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + D-mannitol
beta-D-galactopyranosyl-(1-1)-D-mannitol + 2-nitrophenol
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + D-mannitol
beta-D-galactopyranosyl-(1-1)-D-mannitol + 2-nitrophenol
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
the enzyme displays 4times higher level of activity with 4-nitrophenyl beta-D-galactopyranoside than with 2-nitrophenyl beta-D-galactopyranoside
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
6% activity compared to 4-nitrophenyl alpha-D-galactopyranoside
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
The enzyme hydrolysis of 2-nitrophenyl beta-D-galactopyranoside is linear within 3.5 h reaching 60-65% of substrate conversion, than practically stops
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
100% activity
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
3times lower activity than for 4-nitrophenyl-beta-D-galactopyranoside
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
3times lower activity than for 4-nitrophenyl-beta-D-galactopyranoside
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
Bacillus megaterium KM / ATCC 13632
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
pH 5.0-9.0, 55°C
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
Geobacillus stearothermophilus ATCC 8005
-
pH 5.0-9.0, 55°C
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
low activity
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
pH 6.3, 3 min, 47°C
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
pH 6.3, 3 min, 47°C
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
preferred substrate
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
ir
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
about 83% activity compared to 4-nitrophenyl-beta-D-galactopyranoside
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
about 83% activity compared to 4-nitrophenyl-beta-D-galactopyranoside
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
C5VV31
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
-
61% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
-
72% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + L-sorbose
beta-D-galactopyranosyl-(1-1)-L-sorbose + 2-nitrophenol
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + L-sorbose
beta-D-galactopyranosyl-(1-1)-L-sorbose + 2-nitrophenol
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + salicin
2-(hydroxymethyl)phenyl beta-D-galactopyranosyl-(1->6)-beta-D-glucopyranoside + 2-nitrophenol
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + salicin
2-(hydroxymethyl)phenyl beta-D-galactopyranosyl-(1->6)-beta-D-glucopyranoside + 2-nitrophenol
-
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + beta-D-galactose
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + beta-D-galactose
the enzyme also shows beta-fucosidase activity
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + D-galactose
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + D-galactose
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + D-galactose
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + D-galactose
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + D-galactose
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranose
0.5% of the activity with 2-nitrophenyl beta-D-galactopyranoside
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranose
-
3.27% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
?
4-methylumbelliferyl beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
?
4-methylumbelliferyl beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
?
4-methylumbelliferyl beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
?
4-methylumbelliferyl beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
?
4-methylumbelliferyl beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
18% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
20% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
?
4-nitrophenyl alpha-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
?
4-nitrophenyl alpha-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
beta-galactosidase I: 11.3% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 4.9% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 2.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 8.1% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 6.8% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
3% activity compared to 4-nitrophenyl alpha-D-galactopyranoside
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
relative activity of 125% as compared to 4-nitrophenyl beta-D-glucopyranoside. Maximum hydrolytic activity for 4-nitrophenyl beta-D-fucopyranoside followed by 4-nitrophenyl beta-D-glucopyranoside and 4-nitrophenyl beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
75% of the activity with 4-nitrophenyl beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucose
43% relative to 2-nitrophenyl-beta-D-galactoside hydrolysis
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucose
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
the enzyme displays 4times higher level of activity with 4-nitrophenyl beta-D-galactopyranoside than with 2-nitrophenyl beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
81% activity compared to 4-nitrophenyl alpha-D-galactopyranoside
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
Caldariella acidophila
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
34.49% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
maximum hydrolytic activity for 4-nitrophenyl beta-D-fucopyranoside followed by 4-nitrophenyl beta-D-glucopyranoside and 4-nitrophenyl beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
94% of the activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
preferred substrate, 3times higher activity than for 2-nitrophenyl-beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
preferred substrate, 3times higher activity than for 2-nitrophenyl-beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
Kestingiella geocarpa
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
ir
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
100% activity
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
100% activity
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + beta-D-galactose
-
the enzyme catalyses both hydrolysis and transglycosylation
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + beta-D-galactose
-
the enzyme catalysed both hydrolysis and transglycosylation
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + D-galactose
89% of the activity with 2-nitrophenyl-beta-D-galactoside
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + D-galactose
89% of the activity with 2-nitrophenyl-beta-D-galactoside
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + D-galactose
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + D-galactose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
maximum hydrolytic activity for 4-nitrophenyl beta-D-fucopyranoside followed by 4-nitrophenyl beta-D-glucopyranoside and 4-nitrophenyl beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
71% of the activity with 4-nitrophenyl beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
1.4% of the activity with 2-nitrophenyl beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
0.47% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
0.31% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
11% of the activity with 4-nitrophenyl beta-D-galactopyranoside
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
0.51% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
0.9% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + beta-D-galactose
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + beta-D-galactose
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + beta-D-galactose
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + beta-D-galactose
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + D-galactose
-
43% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + D-galactose
-
56% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
?
-
-
-
?
6-bromo-2-naphthyl-beta-galactoside + H2O
6-bromo-2-naphthol + D-galactose
-
22% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
?
6-bromo-2-naphthyl-beta-galactoside + H2O
6-bromo-2-naphthol + D-galactose
-
30% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
?
a beta-D-galactoside + H2O
D-galactose + an alcohol
-
-
-
?
a beta-D-galactoside + H2O
D-galactose + an alcohol
-
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose
-
-
?
beta-D-(1-6) galactobiose + H2O
?
-
isozymes BbgI, BbgIV, and BbgIII showe minor activity towards beta-D-(1-6) galactobiose (less than 4.7%) in comparison to lactose, whereas isozyme BbgII has a more than 22fold preference for that substrate
-
?
beta-D-(1-6) galactobiose + H2O
?
-
isozymes BbgI, BbgIV, and BbgIII showe minor activity towards beta-D-(1-6) galactobiose (less than 4.7%) in comparison to lactose, whereas isozyme BbgII has a more than 22fold preference for that substrate
-
?
cellobiose + H2O
D-galactose
-
41.7% of the activity with lactose
-
?
lactose + H2O
beta-D-glucose + beta-D-galactose
-
the enzyme is involved in the lactose metabolism of the organism, overview
-
?
lactose + H2O
D-galactose + D-glucose
-
preferred substrate
-
?
lactose + H2O