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Information on EC 3.2.1.23 - beta-galactosidase
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EC Tree
3.2.1.23 beta-galactosidaseIUBMB Comments
Some enzymes in this group hydrolyse alpha-L-arabinosides; some animal enzymes also hydrolyse beta-D-fucosides and beta-D-glucosides; cf. EC 3.2.1.108 lactase.
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Word Map
- 3.2.1.23
- lacz
- adenovirus
- lysosomal
- lactose
- endothelial
- glycoprotein
- bone
- artery
- oligosaccharide
-
histochemical
-
adenovirus-mediated
-
herpes
- beta-glucosidase
- lambda
- beta-glucuronidase
-
retroviral
-
immunoassay
-
foreign
- phage
- luciferase
- gm1
- glycosidases
- neuraminidase
- viruses
- liposome
- cytomegalovirus
- bacteriophage
- simplex
- alpha-glucosidase
- vaccinia
- telomerase
-
adeno-associated
-
galactosylation
-
plaque-forming
-
replication-defective
- alpha-mannosidase
- sialidase
- n-acetyl-beta-glucosaminidase
- alpha-galactosidase
- kluyveromyces
- ganglioside
- medicine
- hexosaminidase
- synthesis
- food industry
- degradation
-
vector-mediated
- biofuel production
- molecular biology
- biotechnology
- p16ink4a
- environmental protection
- diagnostics
- beta-hexosaminidase
- ganciclovir
-
transglycosylation
- nutrition
-
lysogens
- industry
- analysis
- keratan
-
nonviral
The enzyme appears in viruses and cellular organisms
The following organisms could not be found in the NCBI Taxonomy and are therefore not considered for the taxonomic range: Caldariella acidophila ,Kerstingiella geocarpa ,Kestingiella geocarpa ,Paracolobactrum aerogenoides ,Thermoanaerobium sp. ,Torulopsis sphaerica ,Torulopsis sphaerica J28
The following organisms could not be found in the NCBI Taxonomy and are therefore not considered for the taxonomic range: Caldariella acidophila ,Kerstingiella geocarpa ,Kestingiella geocarpa ,Paracolobactrum aerogenoides ,Thermoanaerobium sp. ,Torulopsis sphaerica ,Torulopsis sphaerica J28
Reaction Schemes
Synonyms
Aabeta-gal, Acid beta-galactosidase, B-GAL, BbgI, BbgII, BbgIII, BbgIV, beta galactosidase, beta-D-galactohydrolase, beta-D-galactosidase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Acid beta-galactosidase
BbgI
BbgII
BbgIII
BbgIV
beta-D-galactohydrolase
beta-D-galactoside galactohydrolase
beta-D-glactanase
-
-
-
-
beta-D-lactosidase
-
-
-
-
beta-gal
beta-Gal 1
beta-Gal 2
beta-galactosidase
beta-galase
-
-
-
-
beta-lactosidase
-
-
-
-
betagal
BGA
BgaB
BgaBM
bgaD
BgaH
BgaS
BGL1
BglAp
BGT I
BGT II
cold-active beta-galactosidase
driselase
-
-
-
-
Exo-(1-->4)-beta-D-galactanase
-
-
-
-
exo-beta-(1->3)-D-galactanase
-
-
-
-
GALA
galactosidase
galactosidase, beta
-
-
-
-
Hlac_2868
Hydrolact
-
-
-
-
LacA
LACS
lactase
lactosylceramidase II
-
-
-
-
Lactozym
-
-
-
-
Maxilact
-
-
-
-
MeBglD2
beta-glucosidase/galactosidase/fucosidase activity, cf. 3.2.1.21 and 3.2.1.38
ONPGase
Oryzatym
-
-
-
-
p-nitrophenyl beta-galactosidase
-
-
-
-
S 2107
-
-
-
-
senescence-associated beta-galactosidase
SR12 protein
-
-
-
-
Ss beta-gal
SSO3019
Sumiklat
-
-
-
-
Trilactase
-
-
-
-
XC1214
additional information
Acid beta-galactosidase
-
-
-
-
beta-D-galactoside galactohydrolase
-
-
-
-
lactase
-
-
-
-
additional information
Q0R5R5
the enzyme belongs to the glycosyl hydrolase family 42
additional information
the enzyme belongs to the glycosyl hydrolase family 2
additional information
the enzyme belongs to the glycosyl hydrolase family 2
-
additional information
-
the enzymes LacAand YesZ, and GalA belong to the glycosyl hydrolase families 42 and 43, respectively
additional information
-
the enzyme belongs to the glycosyl hydrolase family 35
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
mechanism
-
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
-
-
-
-
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
catalytic mechanism, active site structure,Glu157 acts as the acid/base in the catalytic reaction
Q0R5R5
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Glu184 acts as acid/base catalyst
-
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
the conserved residues E482, M522, Y523 and E551 are important in catalysis
-
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
mechanism
-
-
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
mechanism
-
-
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
mechanism; mechanism
-
-
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
mechanism
-
-
hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
the conserved residues E482, M522, Y523 and E551 are important in catalysis
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis
hydrolysis of O-glycosyl bond
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
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SYSTEMATIC NAME
IUBMB Comments
beta-D-galactoside galactohydrolase
Some enzymes in this group hydrolyse alpha-L-arabinosides; some animal enzymes also hydrolyse beta-D-fucosides and beta-D-glucosides; cf. EC 3.2.1.108 lactase.
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CAS REGISTRY NUMBER
COMMENTARY
9031-11-2
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2R)-glycerol-O-D-beta-galactopyranoside + H2O
beta-D-galactose + glycerol
-
-
-
-
?
1-naphthyl beta-D-galactopyranoside + H2O
1-naphthol + beta-D-galactopyranose
-
-
-
-
?
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside + H2O
2,4-dinitrophenol + 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside
-
-
-
-
?
2,4-dinitrophenyl-beta-D-galactoside + H2O
2,4-dinitrophenol + beta-D-galactose
-
-
-
-
?
2,4-dinitrophenyl-beta-D-glucoside + H2O
2,4-dinitrophenol + beta-D-glucose
-
activity is 10000 times lower than with 2,4-dinitrophenyl-beta-D-galactoside
-
-
?
2-nitrophenyl alpha-D-galactopyranoside + H2O
2-nitrophenol + alpha-D-galactopyranose
-
1.62% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
2-nitrophenyl beta-D-galactopyranoside + D-mannitol
beta-D-galactopyranosyl-(1-1)-D-mannitol + 2-nitrophenol
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactopyranose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + L-sorbose
beta-D-galactopyranosyl-(1-1)-L-sorbose + 2-nitrophenol
2-nitrophenyl beta-D-galactopyranoside + salicin
2-(hydroxymethyl)phenyl beta-D-galactopyranosyl-(1->6)-beta-D-glucopyranoside + 2-nitrophenol
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranoside
-
-
-
-
?
2-nitrophenyl-alpha-D-mannopyranoside + H2O
2-nitrophenol + alpha-D-mannopyranose
-
0.31% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
2-nitrophenyl-beta-D-fucopyranoside + H2O
2-nitrophenol + beta-D-fucose
Q0R5R5
-
-
-
?
2-phenylethyl-beta-D-galactoside + H2O
2-phenylethanol + beta-D-galactose
-
-
-
-
?
3'-deoxy-5-fluorouridine + beta-D-galactose
3'-deoxy-5'-O-beta-D-galactosyl-5-fluorouridine
-
5-O-beta-D-galactosylfloxuridine is synthesized with a yield of 75% through beta-galactosidase-catalyzed transgalactosylation. Enzyme displays absolute regioselectivity toward the 5'-position of floxuridine. Reaction condition: pH 6.5, 45°C for 60 h
-
-
-
3,4-dinitrophenyl beta-D-galactopyranoside + H2O
3,4-dinitrophenol + beta-D-galactose
-
-
-
-
?
3-nitrophenyl beta-D-galactopyranoside + H2O
3-nitrophenol + beta-D-galactopyranose
-
0.13% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-methylumbelliferyl alpha-L-arabinoside + H2O
4-methylumbelliferone + alpha-L-arabinose
-
-
-
-
?
4-methylumbelliferyl-beta-D-galactopyranoside + H2O
4-methylumbelliferone + beta-D-galactopyranose
-
pH 4.0, 30 min, 37°C
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
100% activity
-
-
?
4-nitrophenyl alpha-D-glucopyranoside + H2O
4-nitrophenol + alpha-D-glucopyranose
7.4% of the activity with 4-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
42% activity compared to 4-nitrophenyl alpha-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-arabinoside + H2O
4-nitrophenol + beta-D-arabinose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
-
low activity
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronic acid
-
0.69% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannopyranose
-
0.9% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-mannoside + H2O
4-nitrophenol + beta-D-mannose
-
-
-
-
?
4-nitrophenyl beta-L-arabinoside + H2O
4-nitrophenol + beta-L-arabinose
-
0.21% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose
-
1.18% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinose
Q0R5R5
-
-
-
?
4-nitrophenyl-alpha-L-arabinoside + H2O
4-nitrophenol + alpha-L-arabinose
-
0.74% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside + H2O
5-bromo-4-chloro-indol + beta-D-galactose
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloro-indol + beta-D-galactose
-
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + beta-D-galactopyranose
-
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + beta-D-galactose
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + D-galactose
5-bromo-4-chloro-3-indolyl-beta-D-galactoside + H2O
5-bromo-4-chloroindol + beta-D-galactose
-
-
-
-
?
5-bromo-4-chloro-3-indoxyl-beta-galactoside + H2O
5-bromo-4-chloro-3-indoxol + beta-D-galactose
-
-
-
-
?
6-bromo-2-naphthyl-beta-galactoside + H2O
6-bromo-2-naphthol + beta-D-galactose
-
-
-
-
?
9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) beta-D-galactopyranoside + H2O
9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-ol) + beta-D-galactose
-
-
-
-
?
9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl)-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl)-beta-D-galactoside + H2O
?
-
assays are performed on individual Escherichia coli beta-galactosidase molecules at 2 different concentrations of the substrate DDAO-beta-D-galactoside using a free zone capillary electrophoresis-based protocol with an in-laboratory-constructed instrument utilizing laser-induced fluorescence detection
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose
-
-
-
?
beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose
-
-
-
?
beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose
-
-
-
?
beta-D-Galp-(1->3)-beta-D-Galp-(1->3)-D-galactitol + H2O
beta-D-Galp-(1->3)-D-galactitol + D-galactopyranose
-
-
-
-
?
beta-D-Galp-(1->6)-beta-D-Galp-(1->6)-D-galactitol + H2O
beta-D-Galp-(1->6)-D-galactitol + D-galactopyranose
-
-
-
-
?
cellobiose + H2O
2 D-glucose
-
41.7% of the activtiy with lactose
-
-
?
chlorophenol red beta-D-galactopyranoside + H2O
chlorophenol red + beta-D-galactose
-
-
-
-
?
chlorophenol-red beta-D-galactopyranoside + H2O
chlorophenol-red + beta-D-galactopyranose
-
-
-
-
?
exopolysaccharide + H2O
beta-D-galactose + ?
-
modified exopolysaccharides produced by Lactococcus lactis subsp. cremonis B39 and B891
-
-
?
fluorescein-beta-D-digalactopyranoside + H2O
fluorescein + beta-D-galactose
-
-
-
-
?
galactan + H2O
?
-
the GHF 42 enzyme LacA can act on the oligosaccharides released by the GH53 enzyme GalA from galactan, overview
-
-
?
galactan + H2O
galactose
-
-
+ small amounts of glucose and arabinose
-
galactotetraose + H2O
beta-D-galactose
-
the GHF 42 enzyme LacA can act on the oligosaccharides released by the GH53 enzyme GalA from galactan, overview
-
-
?
gentiobiose + H2O
2 beta-D-glucose
-
reaction of EC 3.2.1.21, 14.3% of the activtiy with lactose
-
-
?
gentiobiose + H2O
D-galactose + D-glucose
-
14.3% of the activity with lactose
-
-
?
glycoasparagines + H2O
galactose + ?
-
hydrolysis of beta-galactosyl linkages in urinary glycoasparagines
-
?
lactose + benzyloxycarbonyl-Hyp-methyl ester
?
-
transgalactosylation
-
-
?
lactose + benzyloxycarbonyl-Ser-methyl ester
?
-
transgalactosylation
-
-
?
lactose + benzyloxycarbonyl-Thr-methyl ester
?
-
transgalactosylation
-
-
?
lactose + H2O
D-galactose + D-glucose + oligosaccharide
under simulated gastric conditions, BgalA has greater stability (about 100%) and hydrolysis ratio (more than 80%) toward milk lactose than the commercially available beta-galactosidase from Aspergillus oryzae (ATCC 20423)
the main products of lactose hydrolysis by recombinant BgalA are 19.5% D-galactose, 72.5% D-glucose, and 8.0% oligosaccharide
-
?
lactose + myo-inositol
6-beta-O-D-galactopyranosyl myo-inositol + ?
-
-
-
?
lactose + p-nitrophenyl beta-D-glucoside
?
-
transgalactosylation
-
-
?
methyl salicylate beta-D-galactoside + H2O
methyl salicylate + beta-D-galactose
-
-
-
-
?
monogalactosyldiacylglycerol + H2O
galactose + diacylglycerol
-
-
-
?
o-nitrophenyl beta-D-fucopyranoside + H2O
o-nitrophenol + beta-D-fucose
6.1% of the activity with o-nitrophenyl beta-D-galactopyranoside
-
-
?
o-nitrophenyl-beta-D-galactoside + myo-inositol
?
-
transgalactosylation
-
-
?
p-nitrophenyl alpha-arabinofuranoside + H2O
p-nitrophenol + alpha-arabinose
-
weak activity
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-mannopyranoside + H2O
p-nitrophenol + alpha-D-mannose
-
beta-galactosidase I: 0.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 49.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 0.5% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl alpha-L-arabinopyranoside + H2O
p-nitrophenol + alpha-L-arabinopyranose
-
beta-galactosidase I: 20.1% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 32.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 24.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 12.5% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 18.5% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucose
-
beta-galactosidase I: 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 1.1% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 1.2% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 4.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 1.6% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl alpha-L-galactopyranoside + H2O
p-nitrophenol + alpha-L-galactose
-
beta-galactosidase I: 2.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 3.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 3.9% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl beta-D-glucuronide + H2O
p-nitrophenol + ?
-
beta-galactosidase I: 4.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 3.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 15.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 64% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannose
-
beta-galactosidase I: 0.7% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 0.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 0.6% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 4.2% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 0.5% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
p-nitrophenol + N-acetyl-beta-D-glucosamine
-
beta-galactosidase I: 2.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 4.8% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 10.4% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 1.4% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
p-nitrophenyl-beta-D-galactopyranoside + H2O
p-nitrophenol + beta-D-galactose
-
-
-
?
p-nitrophenyl-beta-D-galactoside + alpha-D-methyl-galactoside
?
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + alpha-D-methyl-glucoside
?
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + D-glucose
lactose + p-nitrophenol
-
transgalactosylation
-
?
p-nitrophenyl-beta-D-galactoside + glucose
?
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + myo-inositol
?
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + p-nitrophenyl beta-D-glucoside
?
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + sucrose
?
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-galactoside + xylose
?
-
transgalactosylation
-
-
?
p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + beta-D-glucose
-
60% of the activity with p-nitrophenyl-beta-D-galactoside
-
-
?
pectic polysaccharides + H2O
?
-
1.27% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
phenyl-alpha-D-galactoside + H2O
phenol + alpha-D-galactose
-
14% of the activity with o-nitrophenyl-beta-D-galactoside
-
-
?
thio-(o-nitrophenyl)-beta-D-galactoside + H2O
thio-(o-nitrophenol) + beta-D-galactose
-
-
-
-
?
xyloglucan + H2O
?
-
structural xyloglucans show 0.57% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside, reserve xyloglucans show 1.8% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
1-naphthyl-beta-D-galactoside + H2O
beta-D-galactose + 1-naphthol
-
-
-
-
?
1-naphthyl-beta-D-galactoside + H2O
beta-D-galactose + 1-naphthol
-
-
-
-
?
1-naphthyl-beta-D-glucoside + H2O
beta-D-glucose + 1-naphthol
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranose + H2O
2-nitrophenol + D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranose + H2O
2-nitrophenol + D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + D-mannitol
beta-D-galactopyranosyl-(1-1)-D-mannitol + 2-nitrophenol
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + D-mannitol
beta-D-galactopyranosyl-(1-1)-D-mannitol + 2-nitrophenol
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
the enzyme displays 4times higher level of activity with 4-nitrophenyl beta-D-galactopyranoside than with 2-nitrophenyl beta-D-galactopyranoside
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
6% activity compared to 4-nitrophenyl alpha-D-galactopyranoside
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
The enzyme hydrolysis of 2-nitrophenyl beta-D-galactopyranoside is linear within 3.5 h reaching 60-65% of substrate conversion, than practically stops
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
Lactobacillus plantarum FMNP01
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
100% activity
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
Q0R5R5
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
3times lower activity than for 4-nitrophenyl-beta-D-galactopyranoside
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
3times lower activity than for 4-nitrophenyl-beta-D-galactopyranoside
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
Bacillus megaterium KM / ATCC 13632
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
pH 5.0-9.0, 55°C
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
Geobacillus stearothermophilus ATCC 8005
-
pH 5.0-9.0, 55°C
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
low activity
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
pH 6.3, 3 min, 47°C
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
pH 6.3, 3 min, 47°C
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
preferred substrate
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
ir
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
-
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
-
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
about 83% activity compared to 4-nitrophenyl-beta-D-galactopyranoside
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
about 83% activity compared to 4-nitrophenyl-beta-D-galactopyranoside
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
C5VV31
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
-
61% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
-
72% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + L-sorbose
beta-D-galactopyranosyl-(1-1)-L-sorbose + 2-nitrophenol
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + L-sorbose
beta-D-galactopyranosyl-(1-1)-L-sorbose + 2-nitrophenol
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + salicin
2-(hydroxymethyl)phenyl beta-D-galactopyranosyl-(1->6)-beta-D-glucopyranoside + 2-nitrophenol
-
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + salicin
2-(hydroxymethyl)phenyl beta-D-galactopyranosyl-(1->6)-beta-D-glucopyranoside + 2-nitrophenol
-
-
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + beta-D-galactose
the enzyme also shows beta-fucosidase activity
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + beta-D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + D-galactose
-
-
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + D-galactose
-
-
-
?
2-nitrophenyl beta-D-galactoside + H2O
2-nitrophenol + D-galactose
-
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranose
0.5% of the activity with 2-nitrophenyl beta-D-galactopyranoside
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranose
-
3.27% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-methylumbelliferyl beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
-
?
4-methylumbelliferyl beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
-
?
4-methylumbelliferyl beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
-
?
4-methylumbelliferyl beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
-
?
4-methylumbelliferyl beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
18% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
20% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
-
-
-
-
?
4-nitrophenyl alpha-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
-
?
4-nitrophenyl alpha-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
beta-galactosidase I: 11.3% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase II, 4.9% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase III, 2.0% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase IV, 8.1% of the activity with p-nitrophenyl beta-D-galactopyranoside. beta-Galactosidase V, 6.8% of the activity with p-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
3% activity compared to 4-nitrophenyl alpha-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
relative activity of 125% as compared to 4-nitrophenyl beta-D-glucopyranoside. Maximum hydrolytic activity for 4-nitrophenyl beta-D-fucopyranoside followed by 4-nitrophenyl beta-D-glucopyranoside and 4-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
75% of the activity with 4-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucose
43% relative to 2-nitrophenyl-beta-D-galactoside hydrolysis
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
the enzyme displays 4times higher level of activity with 4-nitrophenyl beta-D-galactopyranoside than with 2-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
81% activity compared to 4-nitrophenyl alpha-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
34.49% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
maximum hydrolytic activity for 4-nitrophenyl beta-D-fucopyranoside followed by 4-nitrophenyl beta-D-glucopyranoside and 4-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
94% of the activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
Q0R5R5
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
preferred substrate, 3times higher activity than for 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
preferred substrate, 3times higher activity than for 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
Kestingiella geocarpa
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
ir
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
100% activity
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
100% activity
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + beta-D-galactose
-
the enzyme catalyses both hydrolysis and transglycosylation
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + beta-D-galactose
-
the enzyme catalysed both hydrolysis and transglycosylation
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + D-galactose
89% of the activity with 2-nitrophenyl-beta-D-galactoside
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + D-galactose
89% of the activity with 2-nitrophenyl-beta-D-galactoside
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + D-galactose
-
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
maximum hydrolytic activity for 4-nitrophenyl beta-D-fucopyranoside followed by 4-nitrophenyl beta-D-glucopyranoside and 4-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
71% of the activity with 4-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
1.4% of the activity with 2-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
0.47% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
0.31% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
11% of the activity with 4-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
0.51% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
0.9% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + beta-D-galactose
-
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + beta-D-galactose
-
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + beta-D-galactose
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + beta-D-galactose
-
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + D-galactose
-
43% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + D-galactose
-
56% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
6-bromo-2-naphthyl-beta-galactoside + H2O
6-bromo-2-naphthol + D-galactose
-
22% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
-
?
6-bromo-2-naphthyl-beta-galactoside + H2O
6-bromo-2-naphthol + D-galactose
-
30% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
-
?
a beta-D-galactoside + H2O
D-galactose + an alcohol
-
-
-
-
?
a beta-D-galactoside + H2O
D-galactose + an alcohol
-
-
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose
-
-
-
?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose
-
-
-
?
beta-D-(1-6) galactobiose + H2O
?
-
isozymes BbgI, BbgIV, and BbgIII showe minor activity towards beta-D-(1-6) galactobiose (less than 4.7%) in comparison to lactose, whereas isozyme BbgII has a more than 22fold preference for that substrate
-
-
?
beta-D-(1-6) galactobiose + H2O
?
-
isozymes BbgI, BbgIV, and BbgIII showe minor activity towards beta-D-(1-6) galactobiose (less than 4.7%) in comparison to lactose, whereas isozyme BbgII has a more than 22fold preference for that substrate
-
-
?
cellobiose + H2O
D-galactose
-
41.7% of the activity with lactose
-
-
?
lactose + H2O
beta-D-glucose + beta-D-galactose
-
the enzyme is involved in the lactose metabolism of the organism, overview
-
-
?
lactose + H2O
beta-D-glucose + beta-D-galactose
Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-
-
?
lactose + H2O
D-galactose + D-glucose
-
preferred substrate
-
-
?
lactose + H2O
D-galactose + D-glucose
-
about 36% activity compared to 4-nitrophenyl-beta-D-galactopyranoside
-
-
?
lactose + H2O
D-galactose + D-glucose
-
about 36% activity compared to 4-nitrophenyl-beta-D-galactopyranoside
-
-
?
lactose + H2O
D-glucose + D-galactose
-
-
-
-
?
lactose + H2O
D-glucose + D-galactose
the enzyme is involved in lactose degradation in milk
-
-
?
lactose + H2O
D-glucose + D-galactose
the enzyme is involved in lactose degradation in milk
-
-
?