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Literature summary for 3.2.1.23 extracted from

  • Patel, G.B.; Mackenzie, C.R.; Agnew, B.J.
    Properties and potential advantages of beta-galactosidase from Bacteroides polypragmatus (1985), Appl. Microbiol. Biotechnol., 22, 114-120.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
Bacteroides polypragmatus
Cys activates Bacteroides polypragmatus
dithiothreitol activates Bacteroides polypragmatus

Inhibitors

Inhibitors Comment Organism Structure
p-nitrophenyl-beta-D-thiogalactoside
-
Bacteroides polypragmatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.43
-
o-nitrophenyl-beta-D-galactoside
-
Bacteroides polypragmatus
9.09
-
lactose
-
Bacteroides polypragmatus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, optimal concentration 5-10 mM Bacteroides polypragmatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
57000 and 103000, 2 protein bands detected, SDS-PAGE Bacteroides polypragmatus
210000 220000 gel filtration Bacteroides polypragmatus

Organism

Organism UniProt Comment Textmining
Bacteroides polypragmatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacteroides polypragmatus

Storage Stability

Storage Stability Organism
5°C to 7°C, 10% v/v gycerol, 20% loss of activity after 4 weeks, complete inactivation in absence of glycerol Bacteroides polypragmatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O
-
Bacteroides polypragmatus D-glucose + D-galactose
-
?
o-nitrophenyl-beta-D-galactoside + H2O
-
Bacteroides polypragmatus o-nitrophenol + beta-D-galactose
-
?

Subunits

Subunits Comment Organism
? 57000 and 103000, 2 protein bands detected, SDS-PAGE Bacteroides polypragmatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
hydrolysis of o-nitrophenyl-beta-D-galactoside Bacteroides polypragmatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 50 35°C: 70% of maximal activity, 50°C: 20% of maximal activity Bacteroides polypragmatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
stable for up to 13 h, 7% loss of activity after 25 h Bacteroides polypragmatus
40
-
stable for 3 h Bacteroides polypragmatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
hydrolysis of o-nitrophenyl-beta-D-galactoside Bacteroides polypragmatus

pH Range

pH Minimum pH Maximum Comment Organism
5.4 7.8 pH 5.4: 7.4% of maximal activity, pH 7.8: 16.2% of maximal activity Bacteroides polypragmatus