Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Bacteroides polypragmatus | |
Cys | activates | Bacteroides polypragmatus | |
dithiothreitol | activates | Bacteroides polypragmatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-nitrophenyl-beta-D-thiogalactoside | - |
Bacteroides polypragmatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
o-nitrophenyl-beta-D-galactoside | - |
Bacteroides polypragmatus | |
9.09 | - |
lactose | - |
Bacteroides polypragmatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, optimal concentration 5-10 mM | Bacteroides polypragmatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
57000 and 103000, 2 protein bands detected, SDS-PAGE | Bacteroides polypragmatus |
210000 | 220000 | gel filtration | Bacteroides polypragmatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides polypragmatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacteroides polypragmatus |
Storage Stability | Organism |
---|---|
5°C to 7°C, 10% v/v gycerol, 20% loss of activity after 4 weeks, complete inactivation in absence of glycerol | Bacteroides polypragmatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | - |
Bacteroides polypragmatus | D-glucose + D-galactose | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Bacteroides polypragmatus | o-nitrophenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 57000 and 103000, 2 protein bands detected, SDS-PAGE | Bacteroides polypragmatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
hydrolysis of o-nitrophenyl-beta-D-galactoside | Bacteroides polypragmatus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 50 | 35°C: 70% of maximal activity, 50°C: 20% of maximal activity | Bacteroides polypragmatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
stable for up to 13 h, 7% loss of activity after 25 h | Bacteroides polypragmatus |
40 | - |
stable for 3 h | Bacteroides polypragmatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
hydrolysis of o-nitrophenyl-beta-D-galactoside | Bacteroides polypragmatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.4 | 7.8 | pH 5.4: 7.4% of maximal activity, pH 7.8: 16.2% of maximal activity | Bacteroides polypragmatus |