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Literature summary for 3.2.1.23 extracted from
- beta-Galactosidase from Aspergillus niger. Separation and characterization of three multiple forms (1979), Eur. J. Biochem., 100, 559-567.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.35 | - |
o-nitrophenyl-beta-D-galactoside | enzyme form 1 | Aspergillus niger |  |
| 2.36 | - |
o-nitrophenyl-beta-D-galactoside | enzyme form 2 | Aspergillus niger | |
| 2.38 | - |
o-nitrophenyl-beta-D-galactoside | enzyme form 3 | Aspergillus niger | |
| 85 | - |
lactose | enzyme form 1 | Aspergillus niger | |
| 105 | - |
lactose | enzyme form 2 | Aspergillus niger | |
| 125 | - |
lactose | enzyme form 3 | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 124000 | - |
enzyme form 1, gel filtration | Aspergillus niger |
| 150000 | - |
enzyme form 2, gel filtration | Aspergillus niger |
| 173000 | - |
enzyme form 3, gel filtration | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | enzyme form 1 contains 13.5% w/w neutral carbohydrate, enzyme form 2 contains 19.2% w/w neutral carbohydrate, enzyme form 3 contains 28.5% w/w neutral carbohydrate | Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 3 enzyme forms | Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 23.1 | - |
enzyme form 1 | Aspergillus niger |
| 77.5 | - |
enzyme form 2 | Aspergillus niger |
| 82.4 | - |
enzyme form 3 | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | - |
Aspergillus niger | D-glucose + D-galactose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Aspergillus niger | o-nitrophenol + beta-D-galactose | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
enzyme form 1, 2 and 3, hydrolysis of lactose | Aspergillus niger |
| 3.2 | - |
enzyme form 3, hydrolysis of o-nitrophenyl-beta-D-galactoside | Aspergillus niger |
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Release 2023.1 (January 2023)
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