Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-dinitrophenyl-2-deoxy-2-chloro-beta-D-galactoside | - |
Escherichia coli | |
2-mercaptoethanol | - |
Escherichia coli | |
citrate | - |
Escherichia coli | |
EDTA | - |
Saccharomyces fragilis | |
EDTA | - |
Streptococcus pneumoniae | |
ethanolamine | - |
Escherichia coli | |
ethylenediamine | - |
Escherichia coli | |
mercaptoethylamine | - |
Escherichia coli | |
n-Propanol | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
2-phenylethyl-beta-D-galactoside | - |
Escherichia coli | |
0.03 | - |
p-nitrophenyl-beta-D-galactoside | - |
Escherichia coli | |
0.12 | - |
2,4-dinitrophenyl-beta-D-galactoside | - |
Escherichia coli | |
0.69 | - |
butyl O-beta-D-galactoside | - |
Escherichia coli | |
2.83 | - |
beta-D-galactosyl azide | - |
Escherichia coli | |
5.5 | - |
lactose | - |
Escherichia coli | |
6.9 | - |
methyl O-beta-galactoside | - |
Escherichia coli | |
8.3 | - |
allolactose | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Rattus norvegicus | 5764 | - |
lysosome | - |
Bos taurus | 5764 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Rattus norvegicus | |
Ca2+ | - |
Kluyveromyces lactis | |
Ca2+ | 0.01 mM, activation at low concentrations in a system containing Na+ at optimal concentration | Escherichia coli | |
Co2+ | - |
Saccharomyces fragilis | |
Co2+ | 0.1 mM, activation in a system containing Na+ at optimal concentration | Escherichia coli | |
Fe2+ | 0.01-0.1 mM, activation in a system containing Na+ at optimal concentration | Escherichia coli | |
K+ | - |
Saccharomyces fragilis | |
K+ | - |
Kluyveromyces lactis | |
K+ | activates hydrolysis of p-nitrophenyl-beta-D-galactoside and p-nitrophenyl alpha-L-arabinoside more effectively than Na+ | Escherichia coli | |
K+ | activates hydrolysis of lactose more effectively than Na+ | Escherichia coli | |
Mg2+ | - |
Saccharomyces fragilis | |
Mg2+ | - |
Kluyveromyces lactis | |
Mg2+ | 0.1-10 mM, activation in a system containing Na+ at optimal concentration | Escherichia coli | |
Mn2+ | - |
Rattus norvegicus | |
Mn2+ | - |
Saccharomyces fragilis | |
Mn2+ | 0.01-0.1 mM, activation in a system containing Na+ at optimal concentration | Escherichia coli | |
Na+ | - |
Rattus norvegicus | |
Na+ | - |
Kluyveromyces lactis | |
Na+ | activates hydrolysis of o-nitrophenyl-beta-D-galactoside more effectively than K+ | Escherichia coli | |
Ni2+ | 0.1 mM, activation in a system containing Na+ at optimal concentration | Escherichia coli | |
Zn2+ | 0.01-0.1 mM, activation in a system containing Na+ at optimal concentration | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
118000 | - |
4 * 118000, equilibrium sedimentation in 8 M urea | Escherichia coli |
135000 | - |
4 * 135000, low speed sedimentation without reaching equilibrium in urea hydrochloride | Streptococcus pneumoniae |
516000 | - |
strain ML 309, calculation from sedimentation and diffusion data | Escherichia coli |
521000 | - |
strain ML 35, calculation from sedimentation and diffusion data | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeromonas caviae | - |
- |
- |
Bacillus subtilis | - |
- |
- |
Bos taurus | - |
- |
- |
Enterobacter cloacae | - |
- |
- |
Escherichia coli | - |
- |
- |
Escherichia coli ML 309 | - |
- |
- |
Escherichia coli ML 35 | - |
- |
- |
Hamamotoa singularis | - |
- |
- |
Helix pomatia | - |
- |
- |
Homo sapiens | - |
- |
- |
Kluyveromyces lactis | - |
- |
- |
Lactococcus lactis | - |
- |
- |
Ovis aries | - |
- |
- |
Paracolobactrum aerogenoides | - |
- |
- |
Priestia megaterium | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Saccharomyces fragilis | - |
- |
- |
Shigella dysenteriae | - |
- |
- |
Streptococcus pneumoniae | - |
- |
- |
Tritrichomonas suis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Rattus norvegicus |
glycoprotein | enzyme from calf intestine contains 88% carbohydrate | Bos taurus |
no modification | enzyme contains no carbohydrate or phosphate | Escherichia coli |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
partial | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
beta-D-galactopyranosyl-(1-4)-beta-D-galactopyranosyl-(1-6)-beta-D-galactopyranosyl-(1-3)-beta-D-galactopyranose + 3 H2O = 4 beta-D-galactopyranose | mechanism | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
brain | - |
Bos taurus | - |
intestine | - |
Homo sapiens | - |
intestine | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
additional information | - |
- |
Bos taurus |
additional information | - |
assay methods | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitrophenyl-beta-D-galactoside + H2O | - |
Escherichia coli | 2,4-dinitrophenol + beta-D-galactose | - |
? | |
2,4-dinitrophenyl-beta-D-glucoside + H2O | activity is 10000 times lower than with 2,4-dinitrophenyl-beta-D-galactoside | Escherichia coli | 2,4-dinitrophenol + beta-D-glucose | - |
? | |
2-phenylethyl-beta-D-galactoside + H2O | - |
Escherichia coli | 2-phenylethanol + beta-D-galactose | - |
? | |
allolactose + H2O | - |
Escherichia coli | D-galactose + D-glucose | - |
? | |
beta-D-galactosyl azide + H2O | - |
Escherichia coli | azide + beta-D-galactose | - |
? | |
beta-D-galactosyl fluoride + H2O | - |
Escherichia coli | fluoride + beta-D-galactose | - |
? | |
butyl O-beta-D-galactoside + H2O | - |
Escherichia coli | butanol + beta-D-galactose | - |
? | |
butyl O-beta-galactoside + H2O | - |
Escherichia coli | butanol + beta-D-galactose | - |
? | |
ethyl-beta-D-galactoside + H2O | - |
Escherichia coli | ethanol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Escherichia coli | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Homo sapiens | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Rattus norvegicus | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Escherichia coli ML 309 | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Escherichia coli ML 35 | D-glucose + D-galactose | - |
? | |
methyl O-beta-galactoside + H2O | - |
Escherichia coli | methanol + beta-D-galactose | - |
? | |
additional information | transgalactosylation with o-nitrophenyl-beta-D-galactoside or phenyl-beta-D-galactoside as galactosyl donor and methanol, glycerol, 2-mercaptoethanol, Tris or 2-phenylethyl alcohol as acceptor | Escherichia coli | ? | - |
? | |
additional information | synthesis of beta-D-galactosides by the direct condensation of free galactose and alcohols | Escherichia coli | ? | - |
? | |
additional information | transgalactosylation with o-nitrophenyl-beta-D-galactoside or phenyl-beta-D-galactoside as galactosyl donor and methanol, glycerol, 2-mercaptoethanol, Tris or 2-phenylethyl alcohol as acceptor | Escherichia coli ML 309 | ? | - |
? | |
additional information | synthesis of beta-D-galactosides by the direct condensation of free galactose and alcohols | Escherichia coli ML 309 | ? | - |
? | |
additional information | transgalactosylation with o-nitrophenyl-beta-D-galactoside or phenyl-beta-D-galactoside as galactosyl donor and methanol, glycerol, 2-mercaptoethanol, Tris or 2-phenylethyl alcohol as acceptor | Escherichia coli ML 35 | ? | - |
? | |
additional information | synthesis of beta-D-galactosides by the direct condensation of free galactose and alcohols | Escherichia coli ML 35 | ? | - |
? | |
p-nitrophenyl alpha-L-arabinoside + H2O | - |
Escherichia coli | p-nitrophenol + alpha-L-arabinose | - |
? | |
p-nitrophenyl alpha-L-arabinoside + H2O | - |
Bos taurus | p-nitrophenol + alpha-L-arabinose | - |
? | |
p-nitrophenyl alpha-L-arabinoside + H2O | - |
Escherichia coli ML 309 | p-nitrophenol + alpha-L-arabinose | - |
? | |
p-nitrophenyl alpha-L-arabinoside + H2O | - |
Escherichia coli ML 35 | p-nitrophenol + alpha-L-arabinose | - |
? | |
p-nitrophenyl-beta-D-galactoside + H2O | - |
Escherichia coli | p-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl-beta-D-galactoside + H2O | - |
Homo sapiens | p-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl-beta-D-galactoside + H2O | - |
Rattus norvegicus | p-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl-beta-D-galactoside + H2O | - |
Bos taurus | p-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl-beta-D-galactoside + H2O | - |
Priestia megaterium | p-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl-beta-D-galactoside + H2O | - |
Escherichia coli ML 309 | p-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl-beta-D-galactoside + H2O | - |
Escherichia coli ML 35 | p-nitrophenol + beta-D-galactose | - |
? | |
phenyl-beta-D-galactoside + H2O | - |
Escherichia coli | phenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 135000, low speed sedimentation without reaching equilibrium in urea hydrochloride | Streptococcus pneumoniae |
tetramer | 4 * 118000, equilibrium sedimentation in 8 M urea | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces fragilis |
additional information | - |
enzyme becomes remarkably heat labile in the presence of fructose 1,6-diphosphate | Escherichia coli |
55 | - |
40 min, in presence of 0.1 M mercaptoethanol and 0.01 MgCl2, 50% loss of activity. Without mercaptoethanol complete loss of activity after 10 min | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26 | - |
beta-D-galactosyl azide | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Saccharomyces fragilis |
6.8 | - |
hydrolysis of o-nitrophenyl-beta-D-galactoside, in presence of 2 mM Mg2+ | Escherichia coli |
7.4 | - |
hydrolysis of o-nitrophenyl-beta-D-galactoside, without Mg2+ | Escherichia coli |