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Literature summary for 3.2.1.23 extracted from

  • Liao, X.; Huang, J.; Zhou, Q.; Guo, L.; Lin, J.; You, L.; Liu, S.; Yang, J.
    Designing of a novel beta-galactosidase for production of functional oligosaccharides (2017), Eur. Food Res. Technol., 243, 979-986 .
No PubMed abstract available
Search for more literature for 3.2.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Lactiplantibacillus plantarum

Protein Variants

Protein Variants Comment Organism
additional information introduction of a second catalytic domain A to give mutant FMNP01Gal-2. Mutant shows almost 3fold increase in specific activity Lactiplantibacillus plantarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.87
-
 2-nitrophenyl beta-D-galactopyranoside mutant FMNP01Gal-2, pH 6.5, 40°C Lactiplantibacillus plantarum
6.68
-
 2-nitrophenyl beta-D-galactopyranoside wild-type, pH 6.5, 40°C Lactiplantibacillus plantarum

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-
Lactiplantibacillus plantarum FMNP01
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.96
-
 wild-type, pH 6.5, 40°C Lactiplantibacillus plantarum
2.62
-
 mutant FMNP01Gal-2, pH 6.5, 40°C Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Lactiplantibacillus plantarum 2-nitrophenol + beta-D-galactopyranose
-
 ?
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Lactiplantibacillus plantarum FMNP01 2-nitrophenol + beta-D-galactopyranose
-
 ?
additional information in the presence of lactose and fructose, wild-type forms two transfer products during transgalactosylation. With mutant FMNP01Gal-2, four transfer products are observed Lactiplantibacillus plantarum ?
-
 ?
additional information in the presence of lactose and fructose, wild-type forms two transfer products during transgalactosylation. With mutant FMNP01Gal-2, four transfer products are observed Lactiplantibacillus plantarum FMNP01 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 75200, wild-type, x * 88220, mutant FMNP01Gal-2, calculated Lactiplantibacillus plantarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Lactiplantibacillus plantarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 4 h, 88% residual activity Lactiplantibacillus plantarum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22.5
-
 2-nitrophenyl beta-D-galactopyranoside wild-type, pH 6.5, 40°C Lactiplantibacillus plantarum
62.7
-
 2-nitrophenyl beta-D-galactopyranoside mutant FMNP01Gal-2, pH 6.5, 40°C Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Lactiplantibacillus plantarum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.36
-
 2-nitrophenyl beta-D-galactopyranoside wild-type, pH 6.5, 40°C Lactiplantibacillus plantarum
21.84
-
 2-nitrophenyl beta-D-galactopyranoside mutant FMNP01Gal-2, pH 6.5, 40°C Lactiplantibacillus plantarum