Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
R109K | the mutant shows decreased specific activity towards 4-nitrophenyl-beta-D-galactopyranoside compared to the wild type enzyme, the apparent Km values toward the natural substrates lactose and lactosucrose are strongly increased | Geobacillus stearothermophilus |
R109V | the mutant shows increased specific activity towards 4-nitrophenyl-beta-D-galactopyranoside compared to the wild type enzyme, the apparent Km values toward the natural substrates lactose and lactosucrose are strongly increased | Geobacillus stearothermophilus |
R109W | the mutant shows increased specific activity towards 4-nitrophenyl-beta-D-galactopyranoside compared to the wild type enzyme, the apparent Km values toward the natural substrates lactose and lactosucrose are strongly increased | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.78 | - |
lactose | wild type enzyme, at 37°C | Geobacillus stearothermophilus | |
5 | - |
lactosucrose | wild type enzyme, at 37°C | Geobacillus stearothermophilus | |
84 | - |
lactose | mutant enzyme R109K, at 37°C | Geobacillus stearothermophilus | |
100 | - |
lactose | mutant enzyme R109V, at 37°C | Geobacillus stearothermophilus | |
114 | - |
lactose | mutant enzyme R109W, at 37°C | Geobacillus stearothermophilus | |
136 | - |
lactosucrose | mutant enzyme R109K, at 37°C | Geobacillus stearothermophilus | |
142 | - |
lactosucrose | mutant enzyme R109V, at 37°C | Geobacillus stearothermophilus | |
160 | - |
lactosucrose | mutant enzyme R109W, at 37°C | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
SDS-PAGE | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus KVE39 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.19 | - |
wild type enzyme, using 20 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C | Geobacillus stearothermophilus |
0.22 | - |
mutant enzyme R109K, using 2.65 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C | Geobacillus stearothermophilus |
0.47 | - |
mutant enzyme R109K, using 20 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C | Geobacillus stearothermophilus |
0.5 | - |
wild type enzyme, using 2.65 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C | Geobacillus stearothermophilus |
0.6 | - |
mutant enzyme R109W, using 2.65 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C | Geobacillus stearothermophilus |
1.52 | - |
mutant enzyme R109V, using 2.65 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C | Geobacillus stearothermophilus |
1.79 | - |
mutant enzyme R109W, using 20 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C | Geobacillus stearothermophilus |
4.9 | - |
mutant enzyme R109V, using 20 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Geobacillus stearothermophilus KVE39 | 4-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Geobacillus stearothermophilus | D-galactose + D-glucose | - |
? | |
lactose + H2O | - |
Geobacillus stearothermophilus KVE39 | D-galactose + D-glucose | - |
? | |
lactosucrose + H2O | - |
Geobacillus stearothermophilus | D-galactose + ? | - |
? | |
lactosucrose + H2O | - |
Geobacillus stearothermophilus KVE39 | D-galactose + ? | - |
? | |
additional information | during the transglycosylation reaction with lactose of the evolved beta-galactosidases, a major trisaccharide, beta-D-galactopyranosyl-(1-3)-beta-D-galactopyranosyl-(1-4)-D-glucopyranoside (3'-galactosyl-lactose) is formed. At the lactose concentration of 18% (w/v), this trisaccharide is obtained in yields of 11.5% (w/w) with BgaB mutant enzyme R109K, 21% (w/w) with BgaB mutant enzyme R109V, 23% (w/w) with BgaB mutant enzyme R109W, and 2% (w/w) with the wild type BgaB enzyme | Geobacillus stearothermophilus | ? | - |
? | |
additional information | during the transglycosylation reaction with lactose of the evolved beta-galactosidases, a major trisaccharide, beta-D-galactopyranosyl-(1-3)-beta-D-galactopyranosyl-(1-4)-D-glucopyranoside (3'-galactosyl-lactose) is formed. At the lactose concentration of 18% (w/v), this trisaccharide is obtained in yields of 11.5% (w/w) with BgaB mutant enzyme R109K, 21% (w/w) with BgaB mutant enzyme R109V, 23% (w/w) with BgaB mutant enzyme R109W, and 2% (w/w) with the wild type BgaB enzyme | Geobacillus stearothermophilus KVE39 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BgaB | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Geobacillus stearothermophilus |