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Literature summary for 3.2.1.23 extracted from
- Purification and some properties of acid beta-galactosidase from Pycnoporus cinnabarinus (1981), J. Ferment. Technol., 59, 325-328.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM CaCl2, 6% inhibition | Trametes cinnabarina |  |
| Co2+ | 10 mM CoCl2, 5% inhibition | Trametes cinnabarina | |
| Cu2+ | 10 mM CuSO4, 45% inhibition | Trametes cinnabarina | |
| D-galactose | - |
Trametes cinnabarina | |
| Hg2+ | 10 mM HgCl2, 12% inhibition | Trametes cinnabarina | |
| lactose | - |
Trametes cinnabarina | |
| Zn2+ | 10 mM ZnSO4, 12% inhibition | Trametes cinnabarina | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.95 | - |
p-nitrophenyl-beta-D-galactoside | - |
Trametes cinnabarina | |
| 33 | - |
lactose | - |
Trametes cinnabarina | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
2 * 58000, SDS-PAGE | Trametes cinnabarina |
| 110000 | - |
gel filtration | Trametes cinnabarina |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes cinnabarina | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trametes cinnabarina |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28 | - |
- |
Trametes cinnabarina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | - |
Trametes cinnabarina | D-glucose + D-galactose | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Trametes cinnabarina | p-nitrophenol + beta-D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 58000, SDS-PAGE | Trametes cinnabarina |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
pH 2.4, 15 min, stable below | Trametes cinnabarina |
| 60 | - |
15 min, complete inactivation | Trametes cinnabarina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.4 | - |
- |
Trametes cinnabarina |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | 37°C, 2 h, stable | Trametes cinnabarina |
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