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Literature summary for 3.2.1.23 extracted from

  • Sekimata, M.; Ogura, K.; Tsumuraya, Y.; Hashimoto, Y.; Yamamoto, S.
    A beta-galactosidase from radish (Raphanus sativus L.) seeds (1989), Plant Physiol., 90, 567-574.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.23

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 0.01%, enhances activity Raphanus sativus

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, almost complete inhibition Raphanus sativus
Cu2+ 1 mM, almost complete inhibition Raphanus sativus
D-arabinose 10 mM, 15% inhibition Raphanus sativus
D-fucose 10 mM, 20% inhibition Raphanus sativus
D-galactal potent, competitive Raphanus sativus
D-galactono-1,4-lactone potent, competitive Raphanus sativus
D-galactose 10 mM D-galactose, 60% inhibition Raphanus sativus
Fe3+ 1 mM, almost complete inhibition Raphanus sativus
Hg2+ 1 mM, almost complete inhibition Raphanus sativus
iodoacetate 1 mM, 52% inhibition Raphanus sativus
L-arabinose 10 mM, 18% inhibition Raphanus sativus
L-fucose 10 mM, 30% inhibition Raphanus sativus
Mn2+ 1 mM, almost complete inhibition Raphanus sativus
PCMB
-
 Raphanus sativus
SDS 0.01%, complete inactivation Raphanus sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46
-
 p-nitrophenyl-beta-D-galactoside
-
 Raphanus sativus
1.19
-
 o-nitrophenyl-beta-D-galactoside
-
 Raphanus sativus
1.27
-
 4-methylumbelliferyl-beta-D-galactoside
-
 Raphanus sativus
4.87
-
 beta-1,3-galactotriose
-
 Raphanus sativus
7.79
-
 beta-1,3-galactobiose
-
 Raphanus sativus
8.55
-
 lactose
-
 Raphanus sativus

Organism

Organism UniProt Comment Textmining
Raphanus sativus
-
 L. var. hortensis cv. Aokubi
-

Purification (Commentary)

Purification (Comment) Organism
-
 Raphanus sativus

Source Tissue

Source Tissue Comment Organism Textmining
radicle epidermal cells, intercellular space of cortical cells, endodermis surrounding a vascular bundle Raphanus sativus
-
seed
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.62
-
-
 Raphanus sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-galactoside + H2O
-
 Raphanus sativus 4-methylumbelliferone + beta-D-galactose
-
 ?
beta-1,3-galactobiose + H2O
-
 Raphanus sativus beta-D-galactose
-
 ?
beta-1,3-galactotriose + H2O
-
 Raphanus sativus ?
-
 ?
beta-1,6-galactobiose + H2O
-
 Raphanus sativus beta-D-galactose
-
 ?
beta-1,6-galactotriose + H2O
-
 Raphanus sativus ?
-
 ?
beta-D-Galp-(1->3)-beta-D-Galp-(1->3)-D-galactitol + H2O
-
 Raphanus sativus beta-D-Galp-(1->3)-D-galactitol + D-galactopyranose
-
 ?
beta-D-Galp-(1->6)-beta-D-Galp-(1->6)-D-galactitol + H2O
-
 Raphanus sativus beta-D-Galp-(1->6)-D-galactitol + D-galactopyranose
-
 ?
lactose + H2O
-
 Raphanus sativus D-glucose + D-galactose
-
 ?
methyl beta-D-galactohexaoside + H2O beta-1,6-linked Raphanus sativus ?
-
 ?
methyl beta-D-galactopentaoside + H2O beta-1,3-linked or beta-1,6-linked Raphanus sativus ?
-
 ?
methyl beta-D-galactotetraoside + H2O beta-1,3-linked Raphanus sativus ?
-
 ?
methyl O-beta-galactoside + H2O
-
 Raphanus sativus methanol + beta-D-galactose
-
 ?
additional information specifically splits off beta-1,3-linked and beta-1,6-linked D-galactosyl residues from the nonreducing ends, their rates of hydrolysis increases with increasing chain length Raphanus sativus ?
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Raphanus sativus o-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O
-
 Raphanus sativus p-nitrophenol + beta-D-galactose
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 4.0, 10 min, stable up to Raphanus sativus
55
-
 pH 4.0, 10 min, complete inactivation Raphanus sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 hydrolysis of p-nitrophenyl-beta-D-galactoside or beta-1,3-galactobiose Raphanus sativus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 8.5 4°C, 24 h, stable Raphanus sativus