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Literature summary for 3.2.1.23 extracted from

  • Kelly, C.; OMahony, M.; Fogarty, W.
    A novel thermostable extracellular beta-galactosidase of Paecilomyces varioti (1988), Appl. Microbiol. Biotechnol., 27, 383-388 .
No PubMed abstract available
Search for more literature for 3.2.1.23

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 10 mM, 72% residual activity Paecilomyces variotii
Cu2+ 10 mM, 72% residual activity Paecilomyces variotii
D-glucono-delta-lactone mixed type inhibition Paecilomyces variotii
Fe2+ 10 mM, 80% residual activity Paecilomyces variotii
Hg2+ 10 mM, no residual activity Paecilomyces variotii
additional information not inhibitory: D-glucose at 0.5 mM, D-galactose at 20 mM Paecilomyces variotii
Ni2+ 10 mM, 72% residual activity Paecilomyces variotii
p-hydroxymercuribenzoate 1 mM, no residual activity Paecilomyces variotii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
64
-
 lactose pH 3.5, 40°C Paecilomyces variotii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Paecilomyces variotii
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
 gel filtration Paecilomyces variotii

Organism

Organism UniProt Comment Textmining
Paecilomyces variotii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paecilomyces variotii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1410
-
 substrate lactose, pH 3.5, 40°C Paecilomyces variotii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Paecilomyces variotii 2-nitrophenol + D-galactopyranose
-
 ?
2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Paecilomyces variotii 2-nitrophenol + D-glucopyranoside
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Paecilomyces variotii 4-nitrophenol + D-galactopyranose
-
 ?
cellobiose + H2O 41.7% of the activtiy with lactose Paecilomyces variotii 2 D-glucose
-
 ?
gentiobiose + H2O reaction of EC 3.2.1.21, 14.3% of the activtiy with lactose Paecilomyces variotii 2 beta-D-glucose
-
 ?
lactose + H2O
-
 Paecilomyces variotii D-galactose + D-glucose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 94000, SDS-PAGE Paecilomyces variotii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Paecilomyces variotii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 3 h, presence of 4% lactose, 95% residual activity Paecilomyces variotii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
-
 Paecilomyces variotii

pI Value

Organism Comment pI Value Maximum pI Value
Paecilomyces variotii isoelectric focusing
-
 4.1

Expression

Organism Comment Expression
Paecilomyces variotii submerged fermentation leads to levels of 73.8 units/ml at 72 h up