Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-D-galactose | product inhibition | Erythrina variegata | |
diethyldicarbonate | - |
Erythrina variegata | |
Hg2+ | complete inhibition at 5 mM | Erythrina variegata | |
additional information | no inhibition by PMSF, DTNB, phenyl glyoxal, N-acetyl imidazole, and N,N'-dicyclohexyl carbodiimide | Erythrina variegata | |
N-bromosuccimide | strong inhibition, attacks a tryptophan residue | Erythrina variegata | |
Zn2+ | complete inhibition at 5 mM | Erythrina variegata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 4.6, 30°C | Erythrina variegata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
gel filtration | Erythrina variegata |
78000 | - |
SDS-PAGE | Erythrina variegata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Erythrina variegata | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme is less glycosylated than other glycosidases | Erythrina variegata |
Purification (Comment) | Organism |
---|---|
native enzyme 135fold by ammonium sulfate fractionation, concanavalin A affinity chromatography, anion and cation exchange chromatography, and gel filtration to homogeneity | Erythrina variegata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Erythrina variegata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
109 | - |
purified enzyme | Erythrina variegata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Erythrina variegata | 4-nitrophenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Erythrina variegata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Erythrina variegata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.4 | - |
- |
Erythrina variegata |