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Literature summary for 3.2.1.23 extracted from

  • Chantarangsee, M.; Tanthanuch, W.; Fujimura, T.; Fry, S.C.; Ketudat Cairns, J.
    Molecular characterization of ?-galactosidases from germinating rice (Oryza sativa) (2007), Plant Sci., 173, 118-134.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA 40% activation at 10 mM of recombinant isozyme OsGal1 Oryza sativa

Cloned(Commentary)

Cloned (Comment) Organism
isozymes OsBGal1 and OsBGal2, DNA and amino acid sequence determination and anaylsis, phylogenetic tree, functional expression of soluble isozymes in Escherichia coli, expression of isozyme OsBGal1 thioredoxin fusion protein Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ complete inhibition at 10 mM of recombinant isozyme OsGal1 Oryza sativa
D-galactose 10% inhibition at 5 mM of recombinant isozyme OsGal1 Oryza sativa
D-gluconolactone 40% inhibition at 10 mM of recombinant isozyme OsGal1 Oryza sativa
delta-galactonolactone 62% inhibition at 10 mM of recombinant isozyme OsGal1 Oryza sativa
Fe3+ complete inhibition at 1 mM of recombinant isozyme OsGal1 Oryza sativa
Hg2+ complete inhibition at 1 mM of recombinant isozyme OsGal1 Oryza sativa
IPTG 38% inhibition at 10 mM of recombinant isozyme OsGal1 Oryza sativa
lactose 15% inhibition at 5 mM of recombinant isozyme OsGal1 Oryza sativa
Mg2+ 27% inhibition at 10 mM of recombinant isozyme OsGal1 Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
4-nitrophenyl-beta-D-galactopyranoside pH 3.5, 60°C, recombinant isozyme OsGal1 Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
x * 97000, isozyme OsGal1, SDS-PAGE Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa Q10RB4 precursor; two isozymes OsBGal1 and OsBGal2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant OsBGal1 thioredoxin fusion protein 23fold from Escherichia coli by nickel affinity and anion exchange chromatography Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
internode
-
Oryza sativa
-
leaf
-
Oryza sativa
-
additional information developmental distribution of isozyme expression in rice tissues, overview Oryza sativa
-
panicle
-
Oryza sativa
-
seed germinating rice, enzyme location in the embryo, aleurone layer and surrounding tissues, and in the radicle and shoot after germination Oryza sativa
-
shoot
-
Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.012
-
recombinant isozyme BGal1 in Escherichia coli, substrate 4-nitrophenyl-beta-D-galactopyranoside Oryza sativa
0.04
-
recombinant isozyme BGal2 in Escherichia coli, substrate 2-nitrophenyl-beta-D-galactopyranoside Oryza sativa
0.058
-
recombinant isozyme BGal1 in Escherichia coli, substrate 2-nitrophenyl-beta-D-galactopyranoside Oryza sativa
0.083
-
recombinant isozyme BGal2 in Escherichia coli, substrate 4-nitrophenyl-beta-D-galactopyranoside Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Oryza sativa 2-nitrophenol + beta-D-galactose
-
ir
4-nitrophenyl beta-D-fucopyranoside + H2O
-
Oryza sativa 4-nitrophenol + beta-D-fucose
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
Oryza sativa 4-nitrophenol + beta-D-galactose
-
ir
additional information the OsBGal1 isozyme releases significant amounts of galactose from 1,3-, 1,4-, and 1,6-beta-linked galactose di- and trisaccharides, with beta-1,3-galactobiose and beta-1,3-galactotriose appearing to be hydrolyzed the fastest, no activity of isozyme OsBGal1 with lactose, as well as with 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-mannoside, 4-nitrophenyl-beta-D-arabinoside, and 4-nitrophenyl-beta-D-xyloside Oryza sativa ?
-
?

Subunits

Subunits Comment Organism
? x * 97000, isozyme OsGal1, SDS-PAGE Oryza sativa

Synonyms

Synonyms Comment Organism
More cf. EC 3.2.1.145 Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60 recombinant isozyme OsGal1 Oryza sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
10 min, purified recombinant isozyme OsGal1, rapid inactivation above Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 3.5 recombinnat isozyme OsGal1 Oryza sativa

pH Range

pH Minimum pH Maximum Comment Organism
2.5 5 inactivation at pH 2.5, half-maximal activity at pH 4.5-5.0, recombinant isozyme OsGal1 Oryza sativa