Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 40% activation at 10 mM of recombinant isozyme OsGal1 | Oryza sativa |
Cloned (Comment) | Organism |
---|---|
isozymes OsBGal1 and OsBGal2, DNA and amino acid sequence determination and anaylsis, phylogenetic tree, functional expression of soluble isozymes in Escherichia coli, expression of isozyme OsBGal1 thioredoxin fusion protein | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | complete inhibition at 10 mM of recombinant isozyme OsGal1 | Oryza sativa | |
D-galactose | 10% inhibition at 5 mM of recombinant isozyme OsGal1 | Oryza sativa | |
D-gluconolactone | 40% inhibition at 10 mM of recombinant isozyme OsGal1 | Oryza sativa | |
delta-galactonolactone | 62% inhibition at 10 mM of recombinant isozyme OsGal1 | Oryza sativa | |
Fe3+ | complete inhibition at 1 mM of recombinant isozyme OsGal1 | Oryza sativa | |
Hg2+ | complete inhibition at 1 mM of recombinant isozyme OsGal1 | Oryza sativa | |
IPTG | 38% inhibition at 10 mM of recombinant isozyme OsGal1 | Oryza sativa | |
lactose | 15% inhibition at 5 mM of recombinant isozyme OsGal1 | Oryza sativa | |
Mg2+ | 27% inhibition at 10 mM of recombinant isozyme OsGal1 | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 3.5, 60°C, recombinant isozyme OsGal1 | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97000 | - |
x * 97000, isozyme OsGal1, SDS-PAGE | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q10RB4 | precursor; two isozymes OsBGal1 and OsBGal2 | - |
Purification (Comment) | Organism |
---|---|
recombinant OsBGal1 thioredoxin fusion protein 23fold from Escherichia coli by nickel affinity and anion exchange chromatography | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
internode | - |
Oryza sativa | - |
leaf | - |
Oryza sativa | - |
additional information | developmental distribution of isozyme expression in rice tissues, overview | Oryza sativa | - |
panicle | - |
Oryza sativa | - |
seed | germinating rice, enzyme location in the embryo, aleurone layer and surrounding tissues, and in the radicle and shoot after germination | Oryza sativa | - |
shoot | - |
Oryza sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.012 | - |
recombinant isozyme BGal1 in Escherichia coli, substrate 4-nitrophenyl-beta-D-galactopyranoside | Oryza sativa |
0.04 | - |
recombinant isozyme BGal2 in Escherichia coli, substrate 2-nitrophenyl-beta-D-galactopyranoside | Oryza sativa |
0.058 | - |
recombinant isozyme BGal1 in Escherichia coli, substrate 2-nitrophenyl-beta-D-galactopyranoside | Oryza sativa |
0.083 | - |
recombinant isozyme BGal2 in Escherichia coli, substrate 4-nitrophenyl-beta-D-galactopyranoside | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Oryza sativa | 2-nitrophenol + beta-D-galactose | - |
ir | |
4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Oryza sativa | 4-nitrophenol + beta-D-fucose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Oryza sativa | 4-nitrophenol + beta-D-galactose | - |
ir | |
additional information | the OsBGal1 isozyme releases significant amounts of galactose from 1,3-, 1,4-, and 1,6-beta-linked galactose di- and trisaccharides, with beta-1,3-galactobiose and beta-1,3-galactotriose appearing to be hydrolyzed the fastest, no activity of isozyme OsBGal1 with lactose, as well as with 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-mannoside, 4-nitrophenyl-beta-D-arabinoside, and 4-nitrophenyl-beta-D-xyloside | Oryza sativa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 97000, isozyme OsGal1, SDS-PAGE | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 3.2.1.145 | Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | recombinant isozyme OsGal1 | Oryza sativa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, purified recombinant isozyme OsGal1, rapid inactivation above | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | 3.5 | recombinnat isozyme OsGal1 | Oryza sativa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 5 | inactivation at pH 2.5, half-maximal activity at pH 4.5-5.0, recombinant isozyme OsGal1 | Oryza sativa |