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Literature summary for 3.2.1.23 extracted from

  • Coker, J.A.; Sheridan, P.P.; Loveland-Curtze, J.; Gutshall, K.R.; Auman, A.J.; Brenchley, J.E.
    Biochemical characterization of a beta-galactosidase with a low temperature optimum obtained from an antarctic Arthrobacter isolate (2003), J. Bacteriol., 185, 5473-5482.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arthrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
D-galactal
-
Arthrobacter sp.
D-galactose
-
Arthrobacter sp.
EDTA 100 mM, inactivation after 1.5 h Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
o-nitrophenyl beta-D-galactopyranoside at 20°C Arthrobacter sp.
0.7
-
o-nitrophenyl beta-D-galactopyranoside at 5°C and at 10°C Arthrobacter sp.
0.8
-
o-nitrophenyl beta-D-galactopyranoside at 18°C Arthrobacter sp.
11.5
-
lactose at 18°C Arthrobacter sp.
19.4
-
lactose at 5°C Arthrobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
K+ slight stimulation Arthrobacter sp.
Mg2+ stimulation Arthrobacter sp.
Mn2+ slight stimulation Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
112000
-
4 * 112000 Arthrobacter sp.
463000
-
analytical ultracentrifugation, at low temperatures, irreversible dissociation into inactive monomers Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. Q7WTU5 antarctic isolate
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O
-
Arthrobacter sp. D-glucose + D-galactose
-
?
o-nitrophenyl beta-D-galactopyranoside + H2O
-
Arthrobacter sp. o-nitrophenol + beta-D-galactose
-
?
p-nitrophenyl beta-D-galactopyranoside + H2O
-
Arthrobacter sp. p-nitrophenol + beta-D-galactose
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 112000 Arthrobacter sp.

Synonyms

Synonyms Comment Organism
BgaS
-
Arthrobacter sp.
H-BgaS His-tagged enzyme form Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
-
Arthrobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
30 0°C: about 50% of maximal activity, 30°C: about 60% of maximal activity Arthrobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.7
-
lactose at 5°C Arthrobacter sp.
5.2
-
lactose at 18°C Arthrobacter sp.
43
-
o-nitrophenyl beta-D-galactopyranoside at 5°C Arthrobacter sp.
57.2
-
o-nitrophenyl beta-D-galactopyranoside at 10°C Arthrobacter sp.
100
-
o-nitrophenyl beta-D-galactopyranoside at 18°C Arthrobacter sp.
110.1
-
o-nitrophenyl beta-D-galactopyranoside at 20°C Arthrobacter sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.218
-
D-galactal
-
Arthrobacter sp.
12
-
D-galactose
-
Arthrobacter sp.