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Literature summary for 3.2.1.23 extracted from

  • Wang, K.; Li, G.; Yu, S.Q.; Zhang, C.T.; Liu, Y.H.
    A novel metagenome-derived beta-galactosidase: gene cloning, overexpression, purification and characterization (2010), Appl. Microbiol. Biotechnol., 88, 155-165.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris as a His-tagged fusion protein uncultured soil bacterium

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ inhibitory effect uncultured soil bacterium
Zn2+ inhibitory effect uncultured soil bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.71
-
2-nitrophenyl beta-D-galactopyranoside pH 7, 38°C uncultured soil bacterium
3.4
-
2-nitrophenyl beta-D-galactopyranoside pH 7, 20°C uncultured soil bacterium
4.7
-
2-nitrophenyl beta-D-galactopyranoside pH 7, 10°C uncultured soil bacterium
6.3
-
2-nitrophenyl beta-D-galactopyranoside pH 7, 5°C uncultured soil bacterium
12.2
-
lactose pH 7, 38°C uncultured soil bacterium
17.6
-
lactose pH 7, 20°C uncultured soil bacterium
27.1
-
lactose pH 7, 10°C uncultured soil bacterium
58.6
-
lactose pH 7, 5°C uncultured soil bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slightly stimulates activity uncultured soil bacterium
K+ slightly stimulates activity uncultured soil bacterium
Na+ slightly stimulates activity uncultured soil bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78600
-
calculated from cDNA uncultured soil bacterium
90000
-
His-tagged fusion protein, SDS-PAGE uncultured soil bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactose + H2O uncultured soil bacterium
-
D-galactose + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
uncultured soil bacterium
-
a metagenomic library from topsoil of Daqing oil field of Heilongjiang Province in China is constructed for the screening of clones with beta-galactosidase activity
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25.4
-
38°C, pH 7, substrate: lactose uncultured soil bacterium
243
-
38°C, pH 7, substrate: 2-nitrophenyl-beta-D-galactopyranoside uncultured soil bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
uncultured soil bacterium 2-nitrophenol + beta-D-galactose
-
?
4-nitrophenyl beta-D-arabinoside + H2O
-
uncultured soil bacterium 4-nitrophenol + beta-D-arabinose
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
uncultured soil bacterium 4-nitrophenol + beta-D-galactose
-
?
4-nitrophenyl beta-D-glucuronide + H2O
-
uncultured soil bacterium 4-nitrophenol + beta-D-glucuronate
-
?
4-nitrophenyl beta-D-mannoside + H2O
-
uncultured soil bacterium 4-nitrophenol + beta-D-mannose
-
?
lactose + H2O
-
uncultured soil bacterium D-galactose + D-glucose
-
?

Synonyms

Synonyms Comment Organism
beta-galactosidase
-
uncultured soil bacterium
ZD410
-
uncultured soil bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
-
uncultured soil bacterium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
20 11% activity at 0°C, 54% activity at 20°C uncultured soil bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 45 ZD410 is stable up to 30°C and has only 17% of its activity at 45°C. It is completely inactivated at 50°C uncultured soil bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 3.7 2-nitrophenyl beta-D-galactopyranoside pH 7, 5°C uncultured soil bacterium
33
-
lactose pH 7, 10°C uncultured soil bacterium
53
-
lactose pH 7, 5°C uncultured soil bacterium
66.7
-
2-nitrophenyl beta-D-galactopyranoside pH 7, 10°C uncultured soil bacterium
92
-
lactose pH 7, 20°C uncultured soil bacterium
141
-
lactose pH 7, 38°C uncultured soil bacterium
149
-
2-nitrophenyl beta-D-galactopyranoside pH 7, 20°C uncultured soil bacterium
386
-
2-nitrophenyl beta-D-galactopyranoside pH 7, 38°C uncultured soil bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
uncultured soil bacterium

pH Range

pH Minimum pH Maximum Comment Organism
6 8 80% activity uncultured soil bacterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.9
-
lactose pH 7, 5°C uncultured soil bacterium
1.21
-
lactose pH 7, 10°C uncultured soil bacterium
3.7
-
2-nitrophenyl beta-D-galactopyranoside pH 7, 5°C uncultured soil bacterium
5.23
-
lactose pH 7, 20°C uncultured soil bacterium
11.6
-
lactose pH 7, 38°C uncultured soil bacterium
14.2
-
2-nitrophenyl beta-D-galactopyranoside pH 7, 10°C uncultured soil bacterium
43.7
-
2-nitrophenyl beta-D-galactopyranoside pH 7, 20°C uncultured soil bacterium
226
-
2-nitrophenyl beta-D-galactopyranoside pH 7, 38°C uncultured soil bacterium