Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris as a His-tagged fusion protein | uncultured soil bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | inhibitory effect | uncultured soil bacterium | |
Zn2+ | inhibitory effect | uncultured soil bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.71 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 7, 38°C | uncultured soil bacterium | |
3.4 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 7, 20°C | uncultured soil bacterium | |
4.7 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 7, 10°C | uncultured soil bacterium | |
6.3 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 7, 5°C | uncultured soil bacterium | |
12.2 | - |
lactose | pH 7, 38°C | uncultured soil bacterium | |
17.6 | - |
lactose | pH 7, 20°C | uncultured soil bacterium | |
27.1 | - |
lactose | pH 7, 10°C | uncultured soil bacterium | |
58.6 | - |
lactose | pH 7, 5°C | uncultured soil bacterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slightly stimulates activity | uncultured soil bacterium | |
K+ | slightly stimulates activity | uncultured soil bacterium | |
Na+ | slightly stimulates activity | uncultured soil bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78600 | - |
calculated from cDNA | uncultured soil bacterium |
90000 | - |
His-tagged fusion protein, SDS-PAGE | uncultured soil bacterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | uncultured soil bacterium | - |
D-galactose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured soil bacterium | - |
a metagenomic library from topsoil of Daqing oil field of Heilongjiang Province in China is constructed for the screening of clones with beta-galactosidase activity | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25.4 | - |
38°C, pH 7, substrate: lactose | uncultured soil bacterium |
243 | - |
38°C, pH 7, substrate: 2-nitrophenyl-beta-D-galactopyranoside | uncultured soil bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
uncultured soil bacterium | 2-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-arabinoside + H2O | - |
uncultured soil bacterium | 4-nitrophenol + beta-D-arabinose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
uncultured soil bacterium | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucuronide + H2O | - |
uncultured soil bacterium | 4-nitrophenol + beta-D-glucuronate | - |
? | |
4-nitrophenyl beta-D-mannoside + H2O | - |
uncultured soil bacterium | 4-nitrophenol + beta-D-mannose | - |
? | |
lactose + H2O | - |
uncultured soil bacterium | D-galactose + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-galactosidase | - |
uncultured soil bacterium |
ZD410 | - |
uncultured soil bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
- |
uncultured soil bacterium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
20 | 11% activity at 0°C, 54% activity at 20°C | uncultured soil bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | ZD410 is stable up to 30°C and has only 17% of its activity at 45°C. It is completely inactivated at 50°C | uncultured soil bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3.7 | 2-nitrophenyl beta-D-galactopyranoside | pH 7, 5°C | uncultured soil bacterium | |
33 | - |
lactose | pH 7, 10°C | uncultured soil bacterium | |
53 | - |
lactose | pH 7, 5°C | uncultured soil bacterium | |
66.7 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 7, 10°C | uncultured soil bacterium | |
92 | - |
lactose | pH 7, 20°C | uncultured soil bacterium | |
141 | - |
lactose | pH 7, 38°C | uncultured soil bacterium | |
149 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 7, 20°C | uncultured soil bacterium | |
386 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 7, 38°C | uncultured soil bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
uncultured soil bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 80% activity | uncultured soil bacterium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
lactose | pH 7, 5°C | uncultured soil bacterium | |
1.21 | - |
lactose | pH 7, 10°C | uncultured soil bacterium | |
3.7 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 7, 5°C | uncultured soil bacterium | |
5.23 | - |
lactose | pH 7, 20°C | uncultured soil bacterium | |
11.6 | - |
lactose | pH 7, 38°C | uncultured soil bacterium | |
14.2 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 7, 10°C | uncultured soil bacterium | |
43.7 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 7, 20°C | uncultured soil bacterium | |
226 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 7, 38°C | uncultured soil bacterium |