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Literature summary for 3.2.1.23 extracted from

  • Gul-Guven, R.; Guven, K.; Poli, A.; Nicolaus, B.
    Purification and some properties of a beta-galactosidase from the thermoacidophilic Alicyclobacillus acidocaldarius subsp. rittmannii isolated from Antarctica (2007), Enzyme Microb. Technol., 40, 1570-1577.
No PubMed abstract available
Search for more literature for 3.2.1.23

Activating Compound

Activating Compound Comment Organism Structure
additional information constitutive enzyme expression with additional 2fold induction by lactose Alicyclobacillus acidocaldarius

Inhibitors

Inhibitors Comment Organism Structure
D-galactose competitive product inhibition Alicyclobacillus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.9
-
 2-nitrophenyl-beta-D-galactopyranoside pH 6.0, 65°C Alicyclobacillus acidocaldarius

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Alicyclobacillus acidocaldarius 5622
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactose + H2O Alicyclobacillus acidocaldarius
-
 D-glucose + D-galactose
-
 ?

Organism

Organism UniProt Comment Textmining
Alicyclobacillus acidocaldarius
-
 subsp. rittmannii, strain MR1, isolated fom Antarctica, a thermoacidophilic bacterium
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 163fold to homogeneity by ammonium sulfate, gel filtration, anion exchange chromatography, ultrafiltration, affinity chromatography, and preparative electrophoresis Alicyclobacillus acidocaldarius

Source Tissue

Source Tissue Comment Organism Textmining
additional information constitutive enzyme expression with additional induction by lactose Alicyclobacillus acidocaldarius
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
113
-
 purified enzyme Alicyclobacillus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Alicyclobacillus acidocaldarius 2-nitrophenol + beta-D-galactose
-
 ?
lactose + H2O
-
 Alicyclobacillus acidocaldarius D-glucose + D-galactose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Alicyclobacillus acidocaldarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.9
-
 2-nitrophenyl-beta-D-galactopyranoside pH 6.0, 65°C Alicyclobacillus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Alicyclobacillus acidocaldarius