Application | Comment | Organism |
---|---|---|
molecular biology | senescence-associated beta-galactosidase activity is a widely used biomarker for assessing replicative sensescence in mammalian cells. Quantitative assay of senescence-associated beta-galactosidase activity in mammalian cell extracts. The assay is capable of detecting relatively subtle changes in activity and confirms that confluency and contact inhibition of growth can cause a small increase in the expression of this biomarker. The assay for measuring senescence-associated changes in beta-galactosidase is suitable for mechanistic studies of senescence regulation in which graduated changes in biomarker expression may be anticipated | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | confluency and contact inhibition of growth can cause a small increase in the expression. Senescent phenyotype is correlated with an increase in total beta-galactosidase | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O | - |
Homo sapiens | ? | - |
? |