Cloned (Comment) | Organism |
---|---|
genes yesZ, lacA and galA, genetic organization, library construction and screening for 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside hydrolyzing activity, expression of His-tagged enzymes in Escherichia coli, cells expressing LacA with GalA gain the ability to use galactan as a carbon source | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of LacA and GalA knockout mutants, the increased beta-galactosidase activity generated in response to the addition of galactan is eliminated by inactivating lacA or galA but unaffected by the inactivation of yesZ, overview | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | recombinant LacA | Bacillus subtilis | |
Cu2+ | recombinant LacA | Bacillus subtilis | |
Zn2+ | recombinant LacA | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | GH53 enzyme GalA | Bacillus subtilis | - |
- |
intracellular | GH42 enzyme LacA | Bacillus subtilis | 5622 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
galactan + H2O | Bacillus subtilis | the GHF 42 enzyme LacA can act on the oligosaccharides released by the GH53 enzyme GalA from galactan, overview | ? | - |
? | |
galactotetraose + H2O | Bacillus subtilis | the GHF 42 enzyme LacA can act on the oligosaccharides released by the GH53 enzyme GalA from galactan, overview | beta-D-galactose | - |
? | |
additional information | Bacillus subtilis | regulation and physiologic functions of LacA and GalA, GH42 and GH53 enzymes, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
genes yesZ, lacA and galA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged LacA from Escherichia coli by nickel affinity chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Bacillus subtilis | 2-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Bacillus subtilis | 4-nitrophenol + beta-D-galactose | - |
? | |
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O | - |
Bacillus subtilis | 5-bromo-4-chloroindol + beta-D-galactose | - |
? | |
galactan + H2O | the GHF 42 enzyme LacA can act on the oligosaccharides released by the GH53 enzyme GalA from galactan, overview | Bacillus subtilis | ? | - |
? | |
galactotetraose + H2O | the GHF 42 enzyme LacA can act on the oligosaccharides released by the GH53 enzyme GalA from galactan, overview | Bacillus subtilis | beta-D-galactose | - |
? | |
additional information | regulation and physiologic functions of LacA and GalA, GH42 and GH53 enzymes, overview | Bacillus subtilis | ? | - |
? | |
additional information | enzyme substrate specificity, overview | Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GALA | - |
Bacillus subtilis |
galactanase | - |
Bacillus subtilis |
LacA | - |
Bacillus subtilis |
More | the enzymes LacAand YesZ, and GalA belong to the glycosyl hydrolase families 42 and 43, respectively | Bacillus subtilis |
YesZ | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant LacA | Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant LacA, stable up to | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | recombinant LacA | Bacillus subtilis |