Protein Variants | Comment | Organism |
---|---|---|
additional information | engineering the Escherichia coli beta-galactosidase for the screening of antiviral protease inhibitors. Identification of a new accomodation site between amino acids 581 and 582, in a solvent-exposed and flexible beta-turn of domain III. the replacement of the model peptide reproducing the matrix-capsid (p17/p24) gag cleavage sequence renders a highly active and efficiently digested chimeric construct. The use of this insertion site, that increases the cleavage potential of this reporter enzyme, can improve the sensitivity and dynamic range of the antiviral drug assay | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00722 | - |
- |