Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1 mM, slight activation (6%), strain L461 | Limosilactobacillus reuteri | |
2-mercaptoethanol | 1 mM, slight activation (9%), strain L103 | Limosilactobacillus reuteri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,4-dithiothreitol | 10 mM, 41% inhibition, strain L103; 10 mM, 58% inhibition, strain L461 | Limosilactobacillus reuteri | |
2-mercaptoethanol | 10 mM, 22% inhibition, strain 461 | Limosilactobacillus reuteri | |
Ca2+ | 1 mM in presence of 10 mM Na+, 30% inhibition, strain L461 | Limosilactobacillus reuteri | |
Cu2+ | 10 mM Cu2+ in presence of 10 mM Na+, complete inhibition, strain L103; 10 mM Cu2+ in presence of 10 mM Na+, complete inhibition, strain L461 | Limosilactobacillus reuteri | |
D-galactose | strain L103; strain L461 | Limosilactobacillus reuteri | |
EDTA | 0.1 mM, 85% inhibition, strain L461; 0.1 mM, 90% inhibition, strain L103 | Limosilactobacillus reuteri | |
Fe2+ | 1 mM in presence of 10 mM Na+, 30% inhibition, strain L461 | Limosilactobacillus reuteri | |
glucose | strain L461 | Limosilactobacillus reuteri | |
Urea | 10 mM, 10% inhibition, strain L103; 10 mM, 11% inhibition, strain L461 | Limosilactobacillus reuteri | |
Zn2+ | 10 mM Cu2+ in presence of 10 mM Na+, complete inhibition, strain L461 | Limosilactobacillus reuteri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.98 | - |
o-nitrophenyl-beta-D-galactoside | pH 6.5, 30°C, strain L461 | Limosilactobacillus reuteri | |
31 | - |
lactose | pH 6.5, 30°C, strain L461 | Limosilactobacillus reuteri | |
60 | - |
lactose | pH 6.5, 30°C, strain L103 | Limosilactobacillus reuteri |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Limosilactobacillus reuteri | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | either 10 mM K+ or 10 mM Na+ is required for maximal activity, strain L103 | Limosilactobacillus reuteri | |
K+ | either 10 mM K+ or 10 mM Na+ is required for maximal activity, strain L461 | Limosilactobacillus reuteri | |
Na+ | either 10 mM K+ or 10 mM Na+ is required for maximal activity, strain L103 | Limosilactobacillus reuteri | |
Na+ | either 10 mM K+ or 10 mM Na+ is required for maximal activity, strain L461 | Limosilactobacillus reuteri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 35000 + 1 * 72000, strain L103, SDS-PAGE | Limosilactobacillus reuteri |
35000 | - |
1 * 35000 + 1 * 72000, strain L461, SDS-PAGE | Limosilactobacillus reuteri |
72000 | - |
1 * 35000 + 1 * 72000, strain L103, SDS-PAGE | Limosilactobacillus reuteri |
72000 | - |
1 * 35000 + 1 * 72000, strain L461, SDS-PAGE | Limosilactobacillus reuteri |
105000 | - |
strain L103, non-denaturing PAGE | Limosilactobacillus reuteri |
105000 | - |
strain L461, non-denaturing PAGE | Limosilactobacillus reuteri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Limosilactobacillus reuteri | - |
- |
- |
Limosilactobacillus reuteri L103 | - |
- |
- |
Limosilactobacillus reuteri L461 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Limosilactobacillus reuteri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
158 | - |
strain L103 | Limosilactobacillus reuteri |
180 | - |
strain L461 | Limosilactobacillus reuteri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-D-galactoside + H2O | - |
Limosilactobacillus reuteri | 4-methylumbelliferone + beta-D-galactose | - |
? | |
4-methylumbelliferyl beta-D-galactoside + H2O | - |
Limosilactobacillus reuteri L461 | 4-methylumbelliferone + beta-D-galactose | - |
? | |
4-methylumbelliferyl beta-D-galactoside + H2O | - |
Limosilactobacillus reuteri L103 | 4-methylumbelliferone + beta-D-galactose | - |
? | |
lactose + H2O | - |
Limosilactobacillus reuteri | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Limosilactobacillus reuteri L461 | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Limosilactobacillus reuteri L103 | D-glucose + D-galactose | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Limosilactobacillus reuteri | o-nitrophenol + beta-D-galactose | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Limosilactobacillus reuteri L461 | o-nitrophenol + beta-D-galactose | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Limosilactobacillus reuteri L103 | o-nitrophenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 35000 + 1 * 72000, strain L103, SDS-PAGE | Limosilactobacillus reuteri |
dimer | 1 * 35000 + 1 * 72000, strain L461, SDS-PAGE | Limosilactobacillus reuteri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
hydrolysis of lactose and o-nitrophenyl-beta-D-galactoside, strain L461 | Limosilactobacillus reuteri |
45 | - |
hydrolysis of lactose, strain L103 | Limosilactobacillus reuteri |
50 | - |
hydrolysis of o-nitrophenyl-beta-D-galactoside, strain L103 | Limosilactobacillus reuteri |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | 20°C: about 55% of maximal activity, 55°C: about 30% of maximal activity, hydrolysis of lactose, strain L461 | Limosilactobacillus reuteri |
20 | 55 | 20°C: about 40% of maximal activity, 55°C: about 50% of maximal activity, hydrolysis of lactose, strain L103 | Limosilactobacillus reuteri |
30 | 60 | 30°C: about 40% of maximal activity, 60°C: about 60% of maximal activity, hydrolysis of o-nitrophenyl-beta-D-galactoside, strain L103 | Limosilactobacillus reuteri |
35 | 60 | 35°C: about 55% of maximal activity, 60°C: about 60% of maximal activity, hydrolysis of o-nitrophenyl-beta-D-galactoside, strain L461 | Limosilactobacillus reuteri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal stability is improved in presence of MgCl2, strain L103 | Limosilactobacillus reuteri |
additional information | - |
thermal stability is improved in presence of MgCl2, strain L461 | Limosilactobacillus reuteri |
25 | - |
pH 6.0, 48 h, about 20% loss of activity, strain L103 | Limosilactobacillus reuteri |
25 | - |
pH 6.5, 48 h, stable below, strain L461 | Limosilactobacillus reuteri |
30 | - |
pH 6.0, 48 h, about 40% loss of activity, strain L103 | Limosilactobacillus reuteri |
30 | - |
pH 6.5, 48 h, about 30% loss of activity, strain L461 | Limosilactobacillus reuteri |
37 | - |
pH 6.0, 48 h, about 70% loss of activity, strain L103 | Limosilactobacillus reuteri |
37 | - |
pH 6.5, 48 h, about 50% loss of activity, strain L461 | Limosilactobacillus reuteri |
45 | - |
pH 6.0, 48 h, complete loss of activity, strain L103 | Limosilactobacillus reuteri |
45 | - |
pH 6.5, 48 h, complete loss of activity, strain L461 | Limosilactobacillus reuteri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
lactose | pH 6.5, 30°C, strain L103 | Limosilactobacillus reuteri | |
58 | - |
lactose | pH 6.5, 30°C, strain L461 | Limosilactobacillus reuteri | |
338 | - |
o-nitrophenyl-beta-D-galactoside | pH 6.5, 30°C, strain L461 | Limosilactobacillus reuteri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
hydrolysis of lactose, strain L461 | Limosilactobacillus reuteri |
8 | - |
hydrolysis of lactose and o-nitrophenyl-beta-D-galactoside, strain L103 | Limosilactobacillus reuteri |
8 | - |
hydrolysis of o-nitrophenyl-beta-D-galactoside, strain L461 | Limosilactobacillus reuteri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.8 | pH 5.5: about 50% of maximal activity, pH 8.8: about 60% of maximal activity, hydrolysis of lactose, strain L461 | Limosilactobacillus reuteri |
6 | 9 | pH 6.0: about 40% of maximal activity, pH 9.0: about 70% of maximal activity, hydrolysis of lactose, strain L103 | Limosilactobacillus reuteri |
6 | 8.5 | pH 6.0: about 40% of maximal activity, pH 8.5: about 60% of maximal activity, hydrolysis of o-nitrophenyl-beta-D-galactoside, strain L103 | Limosilactobacillus reuteri |
6.2 | 8.5 | pH 6.2: about 60% of maximal activity, pH 8.5: about 75% of maximal activity, hydrolysis of o-nitrophenyl-beta-D-galactoside, strain L461 | Limosilactobacillus reuteri |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
37°C, 3 h, about 60% loss of activity, strain L103 | Limosilactobacillus reuteri |
6 | - |
37°C, 10% loss of activity after 3 h, 40% loss of activity after 20 h, strain L103 | Limosilactobacillus reuteri |
6 | 6.5 | enzyme is most stable between pH 6.0 and pH 6.5, strain L461 | Limosilactobacillus reuteri |
7 | - |
37°C, 3 h, complete inactivation, strain L103 | Limosilactobacillus reuteri |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
89 | - |
D-galactose | pH 6.5, 30°C, substrate: lactose, strain L461 | Limosilactobacillus reuteri | |
115 | - |
D-galactose | pH 6.5, 30°C, substrate: o-nitrophenyl-beta-D-galactoside, strain L461 | Limosilactobacillus reuteri | |
188 | - |
D-galactose | pH 6.5, 30°C, substrate: lactose, strain L103 | Limosilactobacillus reuteri | |
683 | - |
D-glucose | pH 6.5, 30°C, substrate: o-nitrophenyl-beta-D-galactoside, strain L461 | Limosilactobacillus reuteri |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Limosilactobacillus reuteri | isoelectric focusing, pH-range 3-10, strain L461 | - |
3.84 |
Limosilactobacillus reuteri | isoelectric focusing, pH-range 3-10, strain L103 | 4.8 | 4.6 |