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Literature summary for 3.2.1.23 extracted from
- Efficient bioconversion of lactose in milk and whey: immobilization and biochemical characterization of a beta-galactosidase from the dairy Streptococcus thermophilus LMD9 strain (2010), Res. Microbiol., 161, 515-525.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Streptococcus thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ni2+ | inhibits activity | Streptococcus thermophilus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 6.5, 25-40°C | Streptococcus thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | enhances stability | Streptococcus thermophilus | |
| Mn2+ | enhances stability | Streptococcus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 118000 | - |
SDS-PAGE | Streptococcus thermophilus |
| 470000 | - |
gel filtration | Streptococcus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | Streptococcus thermophilus | - |
D-glucose + D-galactose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus thermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using gel filtration and anion-exchange chromatography | Streptococcus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 464 | - |
purified enzyme, substrate: 2-nitrophenyl beta-D-galactopyranoside, pH 6.5, 25-40°C | Streptococcus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Streptococcus thermophilus | 2-nitrophenol + beta-D-galactose | - |
? | |
| lactose + H2O | - |
Streptococcus thermophilus | D-glucose + D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 118000 | Streptococcus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-galactosidase | - |
Streptococcus thermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 50 | immobilization of the recombinant Escherichia coli in alginate beads clearly enhances the enzyme activity at various temperatures, including 4 and 50°C, and at pH values from 4.0 to 8.5 | Streptococcus thermophilus |
| 25 | 40 | - |
Streptococcus thermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 234 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 6.5, 25-40°C | Streptococcus thermophilus | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8.5 | immobilization of the recombinant Escherichia coli in alginate beads clearly enhances the enzyme activity at various temperatures, including 4°C and 50°C, and at pH values from 4.0 to 8.5 | Streptococcus thermophilus |
| 6.5 | 7.5 | - |
Streptococcus thermophilus |
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Release 2023.1 (January 2023)
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