Application | Comment | Organism |
---|---|---|
synthesis | immobilization by covalent attachment onto Eupergit C with a binding efficiency of 95%. Immobilization increases both activity and stability at higher pH values and temperature but does not significantly change kinetic parameters for the substrate lactose. The immobilized enzyme shows a strong transgalactosylation reaction, resulting in the formation of galactooligosaccharides. The maximum yield of 34% galactooligosaccharides is obtained when the degree of lactose conversion is roughly 80% | Thermomyces dupontii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-galactose | - |
Thermomyces dupontii | |
D-glucose | - |
Thermomyces dupontii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
2-nitrophenyl beta-D-galactoside | immobilized enzyme, pH 6.5, 40°C | Thermomyces dupontii | |
19 | - |
lactose | native enzyme, pH 6.5, 40°C | Thermomyces dupontii | |
21 | - |
lactose | immobilized enzyme, pH 6.5, 40°C | Thermomyces dupontii | |
35 | - |
2-nitrophenyl beta-D-galactoside | native enzyme, pH 6.5, 40°C | Thermomyces dupontii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces dupontii | - |
- |
- |
Thermomyces dupontii CBS 236.58 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactoside + H2O | - |
Thermomyces dupontii | 2-nitrophenol + beta-D-galactose | - |
? | |
2-nitrophenyl beta-D-galactoside + H2O | - |
Thermomyces dupontii CBS 236.58 | 2-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Thermomyces dupontii | D-galactose + D-glucose | - |
? | |
lactose + H2O | - |
Thermomyces dupontii CBS 236.58 | D-galactose + D-glucose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
native enzyme, substrate 2-nitrophenyl beta-D-galactoside | Thermomyces dupontii |
50 | - |
immobilized enzyme, substrate 2-nitrophenyl beta-D-galactoside | Thermomyces dupontii |
50 | - |
native enzyme, substrate lactose | Thermomyces dupontii |
60 | - |
immobilized enzyme, substrate lactose | Thermomyces dupontii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life 8 h for native enzyme, 27 h for immobilized enzyme, respectively | Thermomyces dupontii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
native enzyme, substrate lactose | Thermomyces dupontii |
6 | - |
immobilized enzyme, and native enzyme with substrate 2-nitrophenyl beta-D-galactoside | Thermomyces dupontii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
58 | - |
D-glucose | immobilized enzyme, pH 6.5, 40°C | Thermomyces dupontii | |
66 | - |
D-glucose | native enzyme, pH 6.5, 40°C | Thermomyces dupontii | |
251 | - |
D-galactose | immobilized enzyme, 2-nitrophenyl beta-D-galactoside, pH 6.5, 40°C | Thermomyces dupontii | |
355 | - |
D-galactose | immobilized enzyme, substrate lactose, pH 6.5, 40°C | Thermomyces dupontii | |
370 | - |
D-galactose | native enzyme, substrate 2-nitrophenyl beta-D-galactoside, pH 6.5, 40°C | Thermomyces dupontii | |
420 | - |
D-galactose | native enzyme, substrate lactose, pH 6.5, 40°C | Thermomyces dupontii |