KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
p-nitrophenyl-beta-D-galactoside | 97000 da enzyme form and 84000 Da enzyme form | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84000 | - |
x * 84000, and a second enzyme form of MW 97000 Da, SDS-PAGE | Rattus norvegicus |
97000 | - |
x * 97000, and a second enzyme form of MW 84000 Da, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | degalactosylation of peanut agglutinin-positive glycoprotein of MW 130000-150000 Da, present in the caput sperm | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the two enzyme forms mainly contain high mannose/hybrid-type, and biantennary complex-type oligosaccharides. The 84000 Da enzyme form contains 13.4 mg sugar per 100 mg enzyme, the 97000 Da enzyme form contains 28.3 mg sugar per 100 mg of enzyme, the 84000 Da enzyme form contains 1.9 mg of N-acetylneuraminic acid per 100 mg enzyme, the 97000 Da enzyme form contains 6.3 mg of N-acetylneuraminic acid per 100 mg enzyme | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
a 84000 Da enzyme form and a 97000 Da enzyme form | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epididymis | epididymal luminal fluid | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, complete inactivation after a few days | Rattus norvegicus |
0-4°C, 0.2-0.3 mg of protein per ml, 10 mM Tris/HCl buffer, 0.1 M NaCl, 0.02% sodium azide, both enzyme forms, stable for 3 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
galactosyl-fetuin + H2O | - |
Rattus norvegicus | galactose + fetuin | - |
? | |
galactosyl-ovomucoid + H2O | - |
Rattus norvegicus | galactose + ovomucoid | - |
? | |
additional information | degalactosylation of peanut agglutinin-positive glycoprotein of MW 130000-150000 Da, present in the caput sperm | Rattus norvegicus | ? | - |
? | |
p-nitrophenyl-beta-D-galactoside + H2O | - |
Rattus norvegicus | p-nitrophenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 84000, and a second enzyme form of MW 97000 Da, SDS-PAGE | Rattus norvegicus |
? | x * 97000, and a second enzyme form of MW 84000 Da, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
hydrolysis of p-nitrophenyl-beta-D-galactoside | Rattus norvegicus |
6.4 | 6.8 | hydrolysis of galactosyl-fetuin | Rattus norvegicus |