Any feedback?
Literature summary for 3.2.1.23 extracted from
- Rational design of the beta-galactosidase from Aspergillus oryzae to improve galactooligosaccharide production (2019), Food Chem., 286, 362-367 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli JM109 cells | Aspergillus oryzae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N140C | the mutant shows improved activity compared to the wild type enzyme | Aspergillus oryzae |
| N140C/W806F | the mutant shows improved activity compared to the wild type enzyme | Aspergillus oryzae |
| W806F | the mutant shows improved activity compared to the wild type enzyme | Aspergillus oryzae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0381 | - |
2-nitrophenyl beta-D-galactoside | mutant enzyme W806F, at pH 5.0 and 60°C | Aspergillus oryzae |  |
| 0.0522 | - |
2-nitrophenyl beta-D-galactoside | wild type enzyme, at pH 5.0 and 60°C | Aspergillus oryzae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | Aspergillus oryzae | - |
beta-D-glucose + beta-D-galactose | - |
? | |
| lactose + H2O | Aspergillus oryzae ATCC 42149 | - |
beta-D-glucose + beta-D-galactose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | Q2UCU3 | - |
- |
| Aspergillus oryzae ATCC 42149 | Q2UCU3 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-galactoside + H2O | - |
Aspergillus oryzae | 2-nitrophenol + beta-D-galactose | - |
? | |
| 2-nitrophenyl beta-D-galactoside + H2O | - |
Aspergillus oryzae ATCC 42149 | 2-nitrophenol + beta-D-galactose | - |
? | |
| lactose + H2O | - |
Aspergillus oryzae | beta-D-glucose + beta-D-galactose | - |
? | |
| lactose + H2O | - |
Aspergillus oryzae ATCC 42149 | beta-D-glucose + beta-D-galactose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-gal | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Aspergillus oryzae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 179.5 | - |
2-nitrophenyl beta-D-galactoside | mutant enzyme W806F, at pH 5.0 and 60°C | Aspergillus oryzae | |
| 224.9 | - |
2-nitrophenyl beta-D-galactoside | wild type enzyme, at pH 5.0 and 60°C | Aspergillus oryzae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus oryzae |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4333 | - |
2-nitrophenyl beta-D-galactoside | wild type enzyme, at pH 5.0 and 60°C | Aspergillus oryzae | |
| 4666 | - |
2-nitrophenyl beta-D-galactoside | mutant enzyme W806F, at pH 5.0 and 60°C | Aspergillus oryzae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
