Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.23 extracted from

  • Taniguchi, A.Y.; Takano, K.
    Purification and properties of b-galactosidase from Tilapia intestine: Digestive enzyme of Tilapia-X (2004), Fish. Sci., 70, 688-694.
No PubMed abstract available
Search for more literature for 3.2.1.23

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 1 mM, 39% inhibition Oreochromis niloticus
CH2ICOOH 1 mM, 50% inhibition Oreochromis niloticus
Cu2+ 1 mM, 16% inhibition Oreochromis niloticus
Hg2+ 1 mM, complete inhibition Oreochromis niloticus
Mg2+ 1 mM, 31% inhibition Oreochromis niloticus
Ni2+ 1 mM, 16% inhibition Oreochromis niloticus
p-chloromercuribenzoate 1 mM, complete inhibition Oreochromis niloticus
Pb2+ 1 mM, 66% inhibition Oreochromis niloticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.25
-
 p-nitrophenyl-beta-D-galactoside
-
 Oreochromis niloticus

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ 1 mM, 30% activation Oreochromis niloticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
 gel filtration Oreochromis niloticus

Organism

Organism UniProt Comment Textmining
Oreochromis niloticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oreochromis niloticus

Source Tissue

Source Tissue Comment Organism Textmining
intestinal juice most of the activity detected in intestinal juice Oreochromis niloticus
-
intestine the enzyme is secreted by the upper and middle parts of the intestine Oreochromis niloticus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
168.6
-
-
 Oreochromis niloticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
 Oreochromis niloticus beta-D-galactose + ?
-
 ?
galactan + H2O
-
 Oreochromis niloticus beta-D-galactose + ?
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O
-
 Oreochromis niloticus p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-glucuronide + H2O 60% of the activity with p-nitrophenyl-beta-D-galactoside Oreochromis niloticus p-nitrophenol + beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 71000, SDS-PAGE Oreochromis niloticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Oreochromis niloticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, stable below Oreochromis niloticus
60
-
 30 min, about 40% loss of activity Oreochromis niloticus
70
-
 30 min, about 70% loss of activity Oreochromis niloticus
80
-
 30 min, about 90% loss of activity Oreochromis niloticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Oreochromis niloticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 30 min, 50% loss of activity Oreochromis niloticus
4
-
 30 min, 30% loss of activity Oreochromis niloticus
5 7 30 min, stable Oreochromis niloticus
8
-
 30 min, 30% loss of activity Oreochromis niloticus
9
-
 30 min, 50% loss of activity Oreochromis niloticus

pI Value

Organism Comment pI Value Maximum pI Value
Oreochromis niloticus electrofocusing, pH-range: 3-10
-
 6.7