Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cetyltrimethylammonium bromide | 148% relative activity at 0.25% (v/v) | Pisum sativum | |
EDTA | 127.3% relative activity at 0.02 mM | Pisum sativum | |
NaCl | 123.47% relative activity at 50 mM | Pisum sativum | |
NaF | 132.65% relative activity at 10 mM | Pisum sativum |
General Stability | Organism |
---|---|
dilute purified BGAL preparation with a protein concentration less than 0.1 mg/ml is unstable with a t1/2 of about 13 h at 4°C and pH 5.0, at a protein concentration of 0.1 mg/ml the enzyme has a t1/2 of about 10 days at 4°C and pH 5.0 | Pisum sativum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 88.29% residual activity at 1 mM | Pisum sativum | |
D-galactose | 50% residual activity at 10 mM | Pisum sativum | |
Mg2+ | 85.18% residual activity at 1 mM | Pisum sativum | |
additional information | not affected by methylmannoside | Pisum sativum | |
SDS | 12.69% residual activity at 0.1% (w/v) | Pisum sativum | |
Triton X-100 | 61% residual activity at 10% (v/v) | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.97 | - |
2-nitrophenyl beta-D-galactopyranoside | in 50 mM glycine-HCl, pH 3.2, at 37°C | Pisum sativum | |
3.03 | - |
lactose | in 50 mM sodium acetate-acetic acid, pH 4.0, at 37°C | Pisum sativum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54200 | - |
deglycosylated enzyme, SDS-PAGE | Pisum sativum |
55000 | - |
purified enzyme, SDS-PAGE | Pisum sativum |
228000 | - |
purified enzyme, at pH 5.0, gel filtration | Pisum sativum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Pisum sativum |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, octyl Sepharose column chromatography, and DEAE-Sephacel gel filtration | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Pisum sativum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.085 | - |
crude extract, at 37°C | Pisum sativum |
77.33 | - |
after 910fold purification, at 37°C | Pisum sativum |
Storage Stability | Organism |
---|---|
4°C, crude preparation, 1 week, the enzyme remains fairly stable with a residual activity of more than 60% | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl alpha-D-galactopyranoside + H2O | 1.62% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 2-nitrophenol + alpha-D-galactopyranose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside + H2O | 100% activity | Pisum sativum | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
2-nitrophenyl beta-D-glucopyranoside + H2O | 3.27% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 2-nitrophenol + D-glucopyranose | - |
? | |
2-nitrophenyl-alpha-D-mannopyranoside + H2O | 0.31% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 2-nitrophenol + alpha-D-mannopyranose | - |
? | |
3-nitrophenyl beta-D-galactopyranoside + H2O | 0.13% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 3-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | 34.49% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | 0.47% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucuronide + H2O | 0.69% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + beta-D-glucuronic acid | - |
? | |
4-nitrophenyl beta-D-mannopyranoside + H2O | 0.9% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + beta-D-mannopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | 0.31% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-L-arabinoside + H2O | 0.21% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + beta-L-arabinose | - |
? | |
4-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | 1.18% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose | - |
? | |
4-nitrophenyl-alpha-D-galactopyranoside + H2O | 0.51% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
4-nitrophenyl-alpha-D-galactopyranoside + H2O | 0.9% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
4-nitrophenyl-alpha-L-arabinoside + H2O | 0.74% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + alpha-L-arabinose | - |
? | |
lactose + H2O | 11.7% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | D-glucose + D-galactose | - |
? | |
pectic polysaccharides + H2O | 1.27% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | ? | - |
? | |
xyloglucan + H2O | structural xyloglucans show 0.57% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside, reserve xyloglucans show 1.8% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BGAL | - |
Pisum sativum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Pisum sativum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | the enzyme retains 86.26% of its activity when kept for 10 min at 40°C however it loses 90.85% of its activity at 50°C and 94.89% at 60°C | Pisum sativum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
55.1 | - |
lactose | in 50 mM sodium acetate-acetic acid, pH 4.0, at 37°C | Pisum sativum | |
293.9 | - |
2-nitrophenyl beta-D-galactopyranoside | in 50 mM glycine-HCl, pH 3.2, at 37°C | Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.2 | - |
for hydrolysis of 2-nitrophenyl beta-D-galactopyranoside | Pisum sativum |
4 | - |
for hydrolysis of lactose | Pisum sativum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
D-galactose | - |
Pisum sativum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pisum sativum | purified enzyme, isoelectric focusing | - |
8.8 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
10 | - |
- |
Pisum sativum | D-galactose |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.18 | - |
lactose | in 50 mM sodium acetate-acetic acid, pH 4.0, at 37°C | Pisum sativum | |
302.9 | - |
2-nitrophenyl beta-D-galactopyranoside | in 50 mM glycine-HCl, pH 3.2, at 37°C | Pisum sativum |