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3.2.1.1: alpha-amylase

This is an abbreviated version!
For detailed information about alpha-amylase, go to the full flat file.

Word Map on EC 3.2.1.1

Reaction

(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose
+
H2O
=
(alpha-D-glucopyranosyl-(1-4))n-m-alpha-D-glucopyranose
+
(alpha-D-glucopyranosyl-(1-4))m-alpha-D-glucopyranose

Synonyms

1,4-alpha-D-glucan glucanohydrolase, 1,4-alpha-D-glucan glucanohydrolase and endoamylase, 1,4-alpha-D-glucan-glucanohydrolase, ABA, acid-stable amylase, acidic amylase, AGXA, AHA, alkaline alpha-amylase, alkalophilic Bacillus alpha-amylase, alpha amylase, alpha amylase 1, alpha-(1,4)-D-glucan glucanohydrolase, alpha-1,4 glucan-glucanohydrolase, alpha-1,4-glucan-4-glucanohydrolase, alpha-1-4 D-glucan glucanohydrolase, alpha-amylase, alpha-amylase 1, alpha-amylase 2, alpha-amylase 3, alpha-amylase A4, alpha-amylase Aasp, alpha-amylase AI, alpha-amylase AOA, Alpha-amylase carcinoid, alpha-amylase CMA, alpha-amylase gt, alpha-amylase HA, alpha-amylase I, alpha-amylase II, alpha-amylase PA, alpha-amylase PPA, alpha-amylase type A isozyme, alpha-amylase type II, alpha-amylase ZSA, alpha-amylases 1, AMF-3, ami, Amy, Amy B, Amy c6, Amy I, Amy II, Amy-1E, amy-CS2, Amy-E, Amy-FC1, AMY1, AMY121, AMY2, Amy3, amy5, Amy7C, AmyA, AmyB, AmyC, AmyCR, AmyD, AmyD-1, AmyE, AmyH, AmyI-1, AmyI3C6, AmyK, AmyK38, AmyL, Amyl III, amylase AI, amylase AII, amylase I, Amylase THC 250, amylase, alpha-, Amylopsin, amylopullulanase, AmyN26, AmyP, AmyQ, AmyS, AmyUS100, AmyUS100DELTAIG, AmyZ2, AOA, AoA1, AoA2, ApkA, Apu, B4168_3135, Ba-amy, BAA, Bacillus licheniformis alpha-amylase, Bactosol TK, barley alpha-amylase 1, BBG7_0117, BGTG-1, BH072alpha-amylase, BHA, BiLA, BLA, Blamy-I, bllj_0710, BMA.2, BSA-2, Bsamy-I, BSTA, Buclamase, Ca2+-independent alpha-amylase gt, CcAmy, CCAP, Clarase, Clone 103, Clone 168, Clone PHV19, Clones GRAMY56 and 963, cold-active alpha-amylase, cold-adapted alpha-amylase, ComA, crustacean cardioactive peptide, diastase, endo-1,4-alpha-D-glucan glucanohydrolase, endo-1,4-alpha-D-glucan glucohydrolase, endo-1,4-alpha-D-glucanohydrolase, endoamylase, FORILASE NTL alpha-amylase, Fortizyme, Fungamyl 800 L, G 995, G6-amylase, GH13Amy-1, GH13Amy-2, glycogenase, Gt-amy, HaAmy1, HaAmy2, haloalkaline alpha-amylase, HAS, HdAmyI, High pI alpha-amylase, HPA, HSA, HSAmy, HSAmy-ar, htur2110, human salivary alpha-amylase, hyperthermophilic alpha-amylase, Isozyme 1B, Kleistase L 1, KRA, LAMY, liquozyme, LLF-alpha-amylase, Low pI alpha-amylase, MalA, maltogenic amylase, maltohexaose-producing alpha-amylase, maltotriose-producing alpha-amylase, MAmy, Maxamyl, Maxilase, Meiotic expression upregulated protein 30, MJA1, More, N8 alpha-amylase, neutral amylase, Pancreatic alpha-amylase, PFTA, Pivozin, PPA, PPA-I, PPA-II, psychrophilic alpha amylase, Ptyalin, raw-starch-digesting alpha-amylase, RB5AMG_01539, Rbamy5, RBLA, RBSA-1, ROAmy, Ruminococcus bromii intermediary alpha-amylase 5, Saci_1162, salt-tolerant alpha-amylase, ScAmy43, Sfamy, Spitase CP 1, SSO1172, SusG, TAA, TaAmy3, Taka-amylase A, Takatherm, TcAmy, TdAmyA, tergal gland protein-1, TfAmy48, Thermamyl, Thermolase, thermostable alpha-amylase, TO_amyl, Tp-AmyS, TVA II, VAAmy1, VAAmy2, VrAmy, ZSA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.1 alpha-amylase

Molecular Weight

Molecular Weight on EC 3.2.1.1 - alpha-amylase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10000
Halalkalibacterium halodurans
-
alpha-amylase I, non-denaturing PAGE
100000
105000
-
calculated from amino acid sequence
119560
-
x * 119560, SDS-PAGE
120000
121000
-
gel filtration
127000
-
gel filtration
129000
-
non-denaturing PAGE
130500
-
equilibrium ultracentrifugation
135000
-
x * 135000, SDS-PAGE
139400
-
gel filtration
14000
-
the difference between the value determined by gel filtration and by nondenaturing gel electrophoresisis is due to hydrophobic interactions between the chromatography matrix and the enzyme molecule, gel filtration
157000
-
gel filtration
160000
-
x * 160000, SDS-PAGE
205000
-
native PAGE
22000
-
gel filtration
22500
24000
-
gel filtration
240000
-
gel filtration
241000
-
recombinant AmyC, gel filtration
25000
-
gel filtration
25000 - 48000
-
gel filtration
26000
27000
-
the difference between the value determined by gel filtration and by SDS-PAGE might be due to the affinity of this enzyme for Sephadex G-100, which might have substrate affinity for the enzyme
28000
-
x * 28000, SDS-PAGE
30000
31000
32500
-
x * 32500, SDS-PAGE
33000
-
1 * 33000, SDS-PAGE
35000
-
SDS-PAGE
40000
-
gel filtration
40900
-
gel filtration
41000
42000
42800
-
x * 42800, SDS-PAGE
43000
-
SDS-PAGE
43500
-
1 * 43500, SDS-PAGE
435000
gel filtration
44000
-
x * 44000, SDS-PAGE
44000 - 48000
45000
45100
x * 45100, calculated from sequence
46000
47000
48000
48000 - 52700
-
equilibrium sedimentation
48138
mass spectrometry
48500
-
enzyme form alpha-III, gel filtration
48700
-
enzyme form AI, sedimentation measurement
48900
-
monomer, sedimentation and diffusion data
49000
49456
x * 49456, calculated from sequence
49600
-
x * 49600, isozyme AI-1, SDS-PAGE, x * 56700, isozyme AI-2, SDS-PAGE, x * 100000, isozyme AII, SDS-PAGE
50000
50000 - 51000
-
sedimentation and diffusion data
50120
calculated from amino acid sequence
50400
Gammarus palustris
-
isoenzyme Amy IC, non-denaturing PAGE
51000
51180
-
gel filtration
51400
-
SDS-PAGE
51544
x * 51544, calculated from sequence
51750
-
x * 51750, calculated from sequence
51948
x * 51948, calculated from sequence
52000
52000 - 53000
-
gel filtration
52480
-
x * 52480, SDS-PAGE
52600
-
equilibrium sedimentation
52700
-
meniscus depletion method
52875
x * 52875, extracellular enzyme, calculation from nucleotide sequence
53000
53600
x * 53600, SDS-PAGE
53700
-
SDS-PAGE
54440
isozyme Amy3, calculated from amino acid sequence
55000
55331
-
x * 55331, deduced from nucleotide sequence, native ABA
55391
-
x * 55391, extracellular enzyme, SDS-PAGE
55400
-
SDS-PAGE
55900
Gammarus palustris
-
isoenzyme Amy II.55, non-denaturing PAGE
56000
56091
-
x * 56091, deduced from nucloeotide sequence
56095
-
x * 56095, mass spectrometry
56133
-
1 * 56133, isoenzymes of family B, SDS-PAGE
56269
-
x * 56269, MARDI-TOF mass spectrometry, non-glycosylated alpha-amylase
56286
-
x * 56286, deduced from nucleotide sequence
56700
57000
57100
Gammarus palustris
-
isoenzyme Amy II.52, non-denaturing PAGE
57900
-
gel filtration
59000
59200
-
recombinant enzyme, gel filtration
59946
-
calculated from sequence
60000
61000
61160
x * 61160, calculated from sequence
61500
-
the difference between the value determined by gel filtration and by nondenaturing gel electrophoresisis is due to hydrophobic interactions between the chromatography matrix and the enzyme molecule, nondenaturing gel electrophoresis
61856
-
1 * 61856, isoenzymes of family A, SDS-PAGE
62000
62060
-
calculated from amino acid sequence
62650
-
x * 62650, SDS-PAGE
62720
electrospray ionisation
62859
-
4 * 62859, AmyC, amino acid sequence calculation, 4 * 62000, recombinant AmyC, SDS-PAGE
63000
-
x * 63000, SDS-PAGE
64000
-
x * 64000, isozyme RBSA-1, SDS-PAGE, x * 47000, isozyme BSA-2, SDS-PAGE, x * 46000, isozyme RBLA, SDS-PAGE
64500
-
deglycosylated enzyme, SDS-PAGE
65000
x * 65000, recombinant enzyme, SDS-PAGE
66000
67000
67500
-
x * 67500, SDS-PAGE
68000
68709
-
x * 68709, amino acid sequence calculation, x * 73000, recombinant His-tagged enzyme, SDS-PAGE
68900
-
x * 68900, recombinant AMY1-SBD fusion enzyme, amino acid sequence calculation, x * 75000, recombinant glycosylated AMY1-SBD fusion enzyme, SDS-PAGE
69200
-
x * 69200, SDS-PAGE
69400
Gammarus palustris
-
isoenzyme Amy III, non-denaturing PAGE
70000
-
1 * 70000, SDS-PAGE
71000
-
excluding the lipoprotein signal peptide
72000
-
gel filtration
72026
x * 72026, calculated from sequence
73000
-
x * 68709, amino acid sequence calculation, x * 73000, recombinant His-tagged enzyme, SDS-PAGE
74000
75000
76000
76084
-
x * 76084, amino acid sequence calculation
79000
-
2 * 79000, SDS-PAGE
80000
-
gel filtration
83000
84000
-
gel filtration
84500
-
gel filtration
87000
89000
-
x * 89000, SDS-PAGE
90000
-
x * 90000, SDS-PAGE
92000
-
x * 92000, SDS-PAGE
94500
-
x * 94500, SDS-PAGE
96000
-
x * 96000, SDS-PAGE
96900
-
dimer, sedimentation and diffusion data
97000
additional information