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10000
Halalkalibacterium halodurans
-
alpha-amylase I, non-denaturing PAGE
105000
-
calculated from amino acid sequence
119560
-
x * 119560, SDS-PAGE
129000
-
non-denaturing PAGE
130500
-
equilibrium ultracentrifugation
135000
-
x * 135000, SDS-PAGE
14000
-
the difference between the value determined by gel filtration and by nondenaturing gel electrophoresisis is due to hydrophobic interactions between the chromatography matrix and the enzyme molecule, gel filtration
160000
-
x * 160000, SDS-PAGE
241000
-
recombinant AmyC, gel filtration
25000 - 48000
-
gel filtration
27000
-
the difference between the value determined by gel filtration and by SDS-PAGE might be due to the affinity of this enzyme for Sephadex G-100, which might have substrate affinity for the enzyme
28000
-
x * 28000, SDS-PAGE
32500
-
x * 32500, SDS-PAGE
33000
-
1 * 33000, SDS-PAGE
42800
-
x * 42800, SDS-PAGE
43500
-
1 * 43500, SDS-PAGE
44000
-
x * 44000, SDS-PAGE
45100
x * 45100, calculated from sequence
48000 - 52700
-
equilibrium sedimentation
48500
-
enzyme form alpha-III, gel filtration
48700
-
enzyme form AI, sedimentation measurement
48900
-
monomer, sedimentation and diffusion data
49456
x * 49456, calculated from sequence
49600
-
x * 49600, isozyme AI-1, SDS-PAGE, x * 56700, isozyme AI-2, SDS-PAGE, x * 100000, isozyme AII, SDS-PAGE
50000 - 51000
-
sedimentation and diffusion data
50120
calculated from amino acid sequence
50400
Gammarus palustris
-
isoenzyme Amy IC, non-denaturing PAGE
51544
x * 51544, calculated from sequence
51750
-
x * 51750, calculated from sequence
51948
x * 51948, calculated from sequence
52000 - 53000
-
gel filtration
52480
-
x * 52480, SDS-PAGE
52600
-
equilibrium sedimentation
52700
-
meniscus depletion method
52875
x * 52875, extracellular enzyme, calculation from nucleotide sequence
53600
x * 53600, SDS-PAGE
54440
isozyme Amy3, calculated from amino acid sequence
55331
-
x * 55331, deduced from nucleotide sequence, native ABA
55391
-
x * 55391, extracellular enzyme, SDS-PAGE
55900
Gammarus palustris
-
isoenzyme Amy II.55, non-denaturing PAGE
56091
-
x * 56091, deduced from nucloeotide sequence
56095
-
x * 56095, mass spectrometry
56133
-
1 * 56133, isoenzymes of family B, SDS-PAGE
56269
-
x * 56269, MARDI-TOF mass spectrometry, non-glycosylated alpha-amylase
56286
-
x * 56286, deduced from nucleotide sequence
57100
Gammarus palustris
-
isoenzyme Amy II.52, non-denaturing PAGE
59200
-
recombinant enzyme, gel filtration
59946
-
calculated from sequence
61160
x * 61160, calculated from sequence
61500
-
the difference between the value determined by gel filtration and by nondenaturing gel electrophoresisis is due to hydrophobic interactions between the chromatography matrix and the enzyme molecule, nondenaturing gel electrophoresis
61856
-
1 * 61856, isoenzymes of family A, SDS-PAGE
62060
-
calculated from amino acid sequence
62650
-
x * 62650, SDS-PAGE
62720
electrospray ionisation
62859
-
4 * 62859, AmyC, amino acid sequence calculation, 4 * 62000, recombinant AmyC, SDS-PAGE
63000
-
x * 63000, SDS-PAGE
64000
-
x * 64000, isozyme RBSA-1, SDS-PAGE, x * 47000, isozyme BSA-2, SDS-PAGE, x * 46000, isozyme RBLA, SDS-PAGE
64500
-
deglycosylated enzyme, SDS-PAGE
65000
x * 65000, recombinant enzyme, SDS-PAGE
67500
-
x * 67500, SDS-PAGE
68709
-
x * 68709, amino acid sequence calculation, x * 73000, recombinant His-tagged enzyme, SDS-PAGE
68900
-
x * 68900, recombinant AMY1-SBD fusion enzyme, amino acid sequence calculation, x * 75000, recombinant glycosylated AMY1-SBD fusion enzyme, SDS-PAGE
69200
-
x * 69200, SDS-PAGE
69400
Gammarus palustris
-
isoenzyme Amy III, non-denaturing PAGE
70000
-
1 * 70000, SDS-PAGE
71000
-
excluding the lipoprotein signal peptide
72026
x * 72026, calculated from sequence
73000
-
x * 68709, amino acid sequence calculation, x * 73000, recombinant His-tagged enzyme, SDS-PAGE
76084
-
x * 76084, amino acid sequence calculation
79000
-
2 * 79000, SDS-PAGE
89000
-
x * 89000, SDS-PAGE
90000
-
x * 90000, SDS-PAGE
92000
-
x * 92000, SDS-PAGE
94500
-
x * 94500, SDS-PAGE
96000
-
x * 96000, SDS-PAGE
96900
-
dimer, sedimentation and diffusion data
100000
gel filtration
100000
-
1 * 100000, SDS-PAGE
100000
4 * 100000, SDS-PAGE
100000
-
x * 49600, isozyme AI-1, SDS-PAGE, x * 56700, isozyme AI-2, SDS-PAGE, x * 100000, isozyme AII, SDS-PAGE
100000
Halalkalibacterium halodurans
-
1 * 100000, alpha-amylase I, SDS-PAGE
120000
-
enzyme form Amyl III, gel filtration
120000
-
2 * 120000, SDS-PAGE
22500
-
gel filtration
22500
-
1 * 22500, SDS-PAGE
26000
-
gel filtration
26000
-
non-denaturing PAGE
30000
-
native PAGE
30000
-
native enzyme, gel filtration
30000
-
gel filtration at high ionic strength
30000
-
isozyme AoA1, gel filtration
31000
-
gel filtration
31000
-
about, native PAGE
41000
-
-
41000
-
approach to equilibium method
42000
-
gel filtration and SDS-PAGE
42000
-
calculation of the molecular weight from relationship of retardation coefficient versus molecular weight of marker proteins
42000
x * 42000, SDS-PAGE
44000 - 48000
-
-
44000 - 48000
Humicola stellata
-
sedimentation measurement
45000
-
gel filtration
45000
-
enzyme form alpha-II, gel filtration
45000
-
gel filtration at low ionic strength
45000
-
isozyme AoA2, gel filtration
45000
-
AMY1 or AMY2, SDS-PAGE
45000
-
x * 45000, SDS-PAGE
46000
-
enzyme form Amyl I, gel filtration
46000
x * 46000, SDS-PAGE
46000
-
1 * 46000, alpha-amylase I, SDS-PAGE
46000
-
x * 64000, isozyme RBSA-1, SDS-PAGE, x * 47000, isozyme BSA-2, SDS-PAGE, x * 46000, isozyme RBLA, SDS-PAGE
46000
-
x * 46000, MALDI-TOF analysis
47000
-
gel filtration
47000
-
monomer, equilibrium sedimentation
47000
-
alpha-amylase I, gel filtration
47000
-
x * 47000, SDS-PAGE
47000
-
x * 47000, SDS-PAGE
47000
-
1 * 47000, SDS-PAGE
47000
-
x * 64000, isozyme RBSA-1, SDS-PAGE, x * 47000, isozyme BSA-2, SDS-PAGE, x * 46000, isozyme RBLA, SDS-PAGE
48000
-
equilibrium sedimentation
48000
SDS-PAGE and gel filtration
48000
native PAGE and gel filtration
48000
-
enzyme form AII, sedimetation measurement
49000
-
-
49000
-
glycerol density gradient centrifugation
49000
-
sedimentation velocity centrifugation
50000
-
gel filtration
50000
Gammarus palustris
-
isoenzyme Amy IW, non-denaturing PAGE
50000
-
x * 50000, SDS-PAGE
51000
-
gel filtration
51000
-
1 * 51000, SDS-PAGE
51000
-
1 * 51000, SDS-PAGE
51000
-
x * 51000, SDS-PAGE
51000
-
x * 51000, isozyme BAA, SDS-PAGE
52000
-
gel filtration
52000
-
enzyme form alpha-I, gel filtration
52000
-
x * 52000, SDS-PAGE
52000
-
x * 52000, SDS-PAGE
52000
-
x * 52000, SDS-PAGE
52000
x * 52000, SDS-PAGE
53000
gel filtration, SDS-PAGE
53000
about, native PAGE, recombinant enzyme
53000
-
x * 53000, SDS-PAGE
53000
x * 53000, SDS-PAGE
53000
-
x * 53000, extracellular enzyme, SDS-PAGE
53000
-
x * 53000, recombinant enzyme, SDS-PAGE
53000
x * 53000, recombinant enzyme, SDS-PAGE
53000
-
x * 53000, SDS-PAGE, intestine alpha-amylase
54000
-
gel filtration
54000
-
enzyme from normal pancreas and from transplantable pancreatic acinar carcinoma, gel filtration
54000
SDS-PAGE, as fusion protein 965000 Da, combined with the maltose binding protein with 42500 Da
54000
-
1 * 54000, SDS-PAGE
54000
-
x * 54000, SDS-PAGE
54000
-
x * 54000, SDS-PAGE
54000
-
x * 54000, pancreatic enzyme, SDS-PAGE
54000
-
x * 54000, SDS-PAGE, recombinant alpha-amylase
54000
-
x * 54000, recombinant wild-type enzyme, SDS-PAGE
55000
-
SDS-PAGE
55000
recombinant enzyme, native PAGE
55000
recombinant His-tagged enzyme, gel filtration
55000
-
1 * 55000, SDS-PAGE
55000
-
x * 55000, SDS-PAGE
55000
-
x * 55000, SDS-PAGE
55000
-
x * 55000, SDS-PAGE
55000
-
x * 55000, non-glycosylated alpha-amylase
56000
-
gel filtration
56000
-
enzyme from pancreas and parotid gland, high-speed equilibrium method
56000
isozyme Amy3, SDS-PAGE
56000
-
1 * 56000, SDS-PAGE
56000
-
1 * 56000, parotid enzyme of enzyme family B, SDS-PAGE
56000
-
x * 62000, glycosylated HSA, SDS-PAGE, x * 56000, deglycosylated HSA, SDS-PAGE
56700
-
x * 49600, isozyme AI-1, SDS-PAGE, x * 56700, isozyme AI-2, SDS-PAGE, x * 100000, isozyme AII, SDS-PAGE
56700
1 * 56700, MALDI-TOF mass spectrometry
57000
-
gel filtration
57000
predicted from amino acid sequence, SDS-PAGE
57000
-
x * 57000, SDS-PAGE
57000
-
x * 57000, SDS-PAGE, native ABA
58000
-
-
58000
-
about, gel filtration
58000
-
acid-stable enzyme, equilibrium sedimentation
58000
-
1 * 58000, SDS-PAGE
58000
-
x * 58000, SDS-PAGE
58000
-
x * 58000, SDS-PAGE
58000
-
x * 58000, SDS-PAGE
59000
-
about, gel filtration
59000
-
enzyme form Amyl II, gel filtration
59000
-
2 * 59000, SDS-PAGE
59000
-
x * 59000, SDS-PAGE
60000
-
SDS-PAGE
60000
-
1 * 60000, SDS-PAGE
61000
-
acid-unstable enzyme, equilibrium sedimentation
61000
-
acid-stable and acid-unstable enzyme, equilibrium sedimentation
61000
-
x * 61000, SDS-PAGE
61000
x * 61000, SDS-PAGE
62000
-
recombinant enzyme AmyN26, SDS-PAGE
62000
-
x * 62000, SDS-PAGE
62000
-
1 * 62000, parotid enzyme of enzyme family A, SDS-PAGE
62000
-
x * 62000, glycosylated alpha-amylase
62000
-
4 * 62859, AmyC, amino acid sequence calculation, 4 * 62000, recombinant AmyC, SDS-PAGE
62000
-
x * 62000, glycosylated HSA, SDS-PAGE, x * 56000, deglycosylated HSA, SDS-PAGE
66000
2 * 66000, SDS-PAGE
66000
-
x * 66000, SDS-PAGE
66000
-
x * 66000, SDS-PAGE
66000
-
x * 66000, SDS-PAGE
66000
-
2 * 66000, intracellular enzyme, electrophoresis in presence of 8 M urea
66000
-
x * 66000, enzyme form II, SDS-PAGE
67000
-
extracellular enzyme, gel filtration
67000
-
x * 67000, SDS-PAGE
67000
-
x * 67000, recombinant enzyme, SDS-PAGE
68000
-
gel filtration
68000
-
native enzyme, SDS-PAGE
68000
-
x * 68000, SDS-PAGE
68000
-
x * 68000, SDS-PAGE
68000
-
x * 68000, SDS-PAGE
74000
-
x * 74000, SDS-PAGE, isoenzyme alpha-I1
74000
-
x * 74000, SDS-PAGE, recombinant ABA, due to glycosylation by Pichia pastoris
75000
-
1 * 75000, SDS-PAGE
75000
-
x * 68900, recombinant AMY1-SBD fusion enzyme, amino acid sequence calculation, x * 75000, recombinant glycosylated AMY1-SBD fusion enzyme, SDS-PAGE
75000
Halalkalibacterium halodurans
-
1 * 75000, alpha-amylase II, SDS-PAGE
76000
-
gel filtration
83000
-
x * 83000, SDS-PAGE, isoenzyme alpha-I2
83000
x * 83000, C-terminally 6*His-tagged recombinant enzyme
87000
Halalkalibacterium halodurans
-
alpha-amylase II, non-denaturing PAGE
87000
-
x * 87000, SDS-PAGE in absence of 2-mercaptoethanol
87000
-
x * 87000, SDS-PAGE in absence of SDS
97000
-
SDS-PAGE
97000
-
x * 97000, SDS-PAGE
additional information
-
3 peaks with alpha-amylase activity are detected by gel filtration
additional information
-
enzyme forms I and II, gel filtration, overview
additional information
-
the modified enzyme TAAMOD acquires a higher molecular weight than the native enzyme TAAUM by 2096 Da