Cloned (Comment) | Organism |
---|---|
hyperexpression in Bacillus subtilis | Bacillus sp. (in: Bacteria) |
Crystallization (Comment) | Organism |
---|---|
recombinant enzyme | Bacillus sp. (in: Bacteria) |
General Stability | Organism |
---|---|
Ca2+, 0.5 mM, and Na+, 12.5 mM, completely protect from thermoinactivation at 60°C | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | no effect | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | catalytic and/or structure-stabilizing Ca2+ ions are tightly bound to the enzyme | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, recombinant enzyme, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) KSM-1378 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Bacillus sp. (in: Bacteria) | additional information | - |
? | |
amylopectin + H2O | - |
Bacillus sp. (in: Bacteria) KSM-1378 | additional information | - |
? | |
amylose + H2O | - |
Bacillus sp. (in: Bacteria) | additional information | - |
? | |
amylose + H2O | - |
Bacillus sp. (in: Bacteria) KSM-1378 | additional information | - |
? | |
maltodextrin + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
maltodextrin + H2O | - |
Bacillus sp. (in: Bacteria) KSM-1378 | ? | - |
? | |
starch + H2O | soluble starch | Bacillus sp. (in: Bacteria) | additional information | the major products are maltotriose + maltopentaose | ? | |
starch + H2O | soluble starch | Bacillus sp. (in: Bacteria) KSM-1378 | additional information | the major products are maltotriose + maltopentaose | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, recombinant enzyme, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
LAMY | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | recombinant enzyme | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
20 min, recombinant enzyme, stable up to | Bacillus sp. (in: Bacteria) |
60 | - |
20 min, recombinant enzyme, complete loss of activity, Ca2+, 0.5 mM, and Na+, 12.5 mM, completely protect from thermoinactivation | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Bacillus sp. (in: Bacteria) |