Protein Variants | Comment | Organism |
---|---|---|
D231N | the specific activity for the mutant enzyme D233N is decreased by 6.3% compared to the wild type. There are no significant changes in the Km value, thermostability, optimum temperature, and optimum pH | Bacillus amyloliquefaciens |
D233N | the specific activity for the mutant enzyme D233N is decreased by 84.8% compared to the wild type. D233N exhibits 56% increase in Km and 85.1% decrease in kcat, thermostability at 60°C, optimum temperature and optimum pH for D233N ae reduced to about 10°C and 3-4 units, respectively | Bacillus amyloliquefaciens |
D438G | the specific activity for the mutant enzyme D233N is decreased by 3.5% compared to the wild type. There are no significant changes in the Km value, thermostability, optimum temperature, and optimum pH | Bacillus amyloliquefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25.1 | - |
starch | mutant enzyme D233N | Bacillus amyloliquefaciens | |
157 | - |
starch | mutant enzyme D231N | Bacillus amyloliquefaciens | |
161.7 | - |
starch | mutant enzyme D438G | Bacillus amyloliquefaciens | |
167.6 | - |
starch | wild type enzyme | Bacillus amyloliquefaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the Ca2+-binding residue Asp233 affects significantly the alpha-amylase specific activity | Bacillus amyloliquefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
SDS-PAGE | Bacillus amyloliquefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Bacillus amyloliquefaciens | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Bacillus amyloliquefaciens CICIM B2125 | - |
malto-oligosaccharides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | P00692 | - |
- |
Bacillus amyloliquefaciens CICIM B2125 | P00692 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Bacillus amyloliquefaciens | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Bacillus amyloliquefaciens CICIM B2125 | malto-oligosaccharides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AmyQ | - |
Bacillus amyloliquefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 75 | in presence of 5 mM Ca2+, the optimum temperature of AmyQ is between 50 and 75°C | Bacillus amyloliquefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
AmyQ remains stable at 60°C for 30 min | Bacillus amyloliquefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | - |
Bacillus amyloliquefaciens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | - |
Bacillus amyloliquefaciens |