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Literature summary for 3.2.1.1 extracted from

  • Yang, C.; Huang, Y.; Chen, C.; Wen, C.
    Expression of Thermobifida fusca thermostable raw starch digesting alpha-amylase in Pichia pastoris and its application in raw sago starch hydrolysis (2010), J. Ind. Microbiol. Biotechnol., 37, 401-406.
    View publication on PubMed
Search for more literature for 3.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
high level recombinant expression in Pichia pastoris X-33 host strain using vector pGAPZaA, allowing constitutive expression and secretion of the protein Thermobifida fusca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, recombinant enzyme, SDS-PAGE Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca Q1KLC8 isolated from compost soils collected in Taiwan
-
Thermobifida fusca NTU22 Q1KLC8 isolated from compost soils collected in Taiwan
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
raw sago starch + H2O high level of activity Thermobifida fusca ?
-
 ?
raw sago starch + H2O high level of activity Thermobifida fusca NTU22 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, recombinant enzyme, SDS-PAGE Thermobifida fusca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Thermobifida fusca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 purified recombinant enzyme, 3 h, 75% activity remaining Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Thermobifida fusca

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 purified recombinant enzyme, 4°C, 24 h, stable Thermobifida fusca