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Literature summary for 3.2.1.1 extracted from

  • Zhang, J.W.; Zeng, R.Y.
    Purification and characterization of a cold-adapted alpha-amylase produced by Nocardiopsis sp. 7326 isolated from Prydz Bay, Antarctic (2008), Mar. Biotechnol., 10, 75-82.
    View publication on PubMed
Search for more literature for 3.2.1.1

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ 5 mM, 1.15fold activation Nocardiopsis sp.
Co2+ 5 mM, 1.39fold activation Nocardiopsis sp.
Cu2+ 5 mM, 1.42fold activation Nocardiopsis sp.
Mg2+ 5 mM, 1.13fold activation Nocardiopsis sp.
Mn2+ 5 mM, 1.43fold activation Nocardiopsis sp.

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, 15% inhibition Nocardiopsis sp.
Hg2+ 5 mM, 28% inhibition Nocardiopsis sp.
Rb+ 5 mM, 33% inhibition Nocardiopsis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, SDS-PAGE Nocardiopsis sp.

Organism

Organism UniProt Comment Textmining
Nocardiopsis sp.
-
-
-
Nocardiopsis sp. 7326
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nocardiopsis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
548
-
-
 Nocardiopsis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble starch + H2O
-
 Nocardiopsis sp. glucose + maltose + maltotriose
-
 ?
soluble starch + H2O
-
 Nocardiopsis sp. 7326 glucose + maltose + maltotriose
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Nocardiopsis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Nocardiopsis sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 50 0°C: about 25% of maximal activity, 50°C: about 40% of maximal activity Nocardiopsis sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 30 min, 82% loss of activity Nocardiopsis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Nocardiopsis sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.6 10 pH 5.6: about 30% of maximal activity, pH 10.0: about 40% of maximal activity Nocardiopsis sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 4°C, 24 h, stable Nocardiopsis sp.