3.2.1.1: alpha-amylase
This is an abbreviated version!
For detailed information about alpha-amylase, go to the full flat file.
Word Map on EC 3.2.1.1
-
3.2.1.1
-
cortisol
-
wheat
-
aspergillus
-
maltose
-
pancreas
-
glycogen
-
oryzae
-
granule
-
lipase
-
alpha-glucosidase
-
acid-schiff
-
flour
-
glucoamylase
-
amylolytic
-
amylose
-
aleurone
-
grain
-
sympathetic
-
social
-
parotid
-
cereal
-
acarbose
-
amyloliquefaciens
-
psychological
-
anxiety
-
amylopectin
-
beta-amylase
-
stearothermophilus
-
honey
-
gibberellic
-
endosperm
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phaseolus
-
psychosocial
-
bread
-
hypothalamic-pituitary-adrenal
-
alcian
-
bake
-
cyclodextrins
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dextrin
-
amyloglucosidase
-
pas-positive
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transglycosylation
-
baking
-
pullulanase
-
debranching
-
dough
-
isoamylase
-
awakening
-
eclosion
-
pullulan
-
brewing
-
nutrition
-
synthesis
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biotechnology
-
pharmacology
-
analysis
-
medicine
-
industry
-
food industry
-
energy production
-
biofuel production
-
detergent
-
agriculture
- 3.2.1.1
- cortisol
- wheat
- aspergillus
- maltose
- pancreas
- glycogen
- oryzae
- granule
- lipase
- alpha-glucosidase
-
acid-schiff
- flour
- glucoamylase
-
amylolytic
- amylose
- aleurone
- grain
-
sympathetic
-
social
- parotid
- cereal
- acarbose
- amyloliquefaciens
-
psychological
-
anxiety
- amylopectin
- beta-amylase
- stearothermophilus
- honey
-
gibberellic
- endosperm
- phaseolus
-
psychosocial
-
bread
-
hypothalamic-pituitary-adrenal
-
alcian
-
bake
- cyclodextrins
- dextrin
- amyloglucosidase
-
pas-positive
-
transglycosylation
-
baking
- pullulanase
-
debranching
-
dough
- isoamylase
- awakening
-
eclosion
- pullulan
- brewing
- nutrition
- synthesis
- biotechnology
- pharmacology
- analysis
- medicine
- industry
- food industry
- energy production
- biofuel production
- detergent
- agriculture
Reaction
Synonyms
1,4-alpha-D-glucan glucanohydrolase, 1,4-alpha-D-glucan glucanohydrolase and endoamylase, 1,4-alpha-D-glucan-glucanohydrolase, ABA, acid-stable amylase, acidic amylase, AGXA, AHA, alkaline alpha-amylase, alkalophilic Bacillus alpha-amylase, alpha amylase, alpha amylase 1, alpha-(1,4)-D-glucan glucanohydrolase, alpha-1,4 glucan-glucanohydrolase, alpha-1,4-glucan-4-glucanohydrolase, alpha-1-4 D-glucan glucanohydrolase, alpha-amylase, alpha-amylase 1, alpha-amylase 2, alpha-amylase 3, alpha-amylase A4, alpha-amylase Aasp, alpha-amylase AI, alpha-amylase AOA, Alpha-amylase carcinoid, alpha-amylase CMA, alpha-amylase gt, alpha-amylase HA, alpha-amylase I, alpha-amylase II, alpha-amylase PA, alpha-amylase PPA, alpha-amylase type A isozyme, alpha-amylase type II, alpha-amylase ZSA, alpha-amylases 1, AMF-3, ami, Amy, Amy B, Amy c6, Amy I, Amy II, Amy-1E, amy-CS2, Amy-E, Amy-FC1, AMY1, AMY121, AMY2, Amy3, amy5, Amy7C, AmyA, AmyB, AmyC, AmyCR, AmyD, AmyD-1, AmyE, AmyH, AmyI-1, AmyI3C6, AmyK, AmyK38, AmyL, Amyl III, amylase AI, amylase AII, amylase I, Amylase THC 250, amylase, alpha-, Amylopsin, amylopullulanase, AmyN26, AmyP, AmyQ, AmyS, AmyUS100, AmyUS100DELTAIG, AmyZ2, AOA, AoA1, AoA2, ApkA, Apu, B4168_3135, Ba-amy, BAA, Bacillus licheniformis alpha-amylase, Bactosol TK, barley alpha-amylase 1, BBG7_0117, BGTG-1, BH072alpha-amylase, BHA, BiLA, BLA, Blamy-I, bllj_0710, BMA.2, BSA-2, Bsamy-I, BSTA, Buclamase, Ca2+-independent alpha-amylase gt, CcAmy, CCAP, Clarase, Clone 103, Clone 168, Clone PHV19, Clones GRAMY56 and 963, cold-active alpha-amylase, cold-adapted alpha-amylase, ComA, crustacean cardioactive peptide, diastase, endo-1,4-alpha-D-glucan glucanohydrolase, endo-1,4-alpha-D-glucan glucohydrolase, endo-1,4-alpha-D-glucanohydrolase, endoamylase, FORILASE NTL alpha-amylase, Fortizyme, Fungamyl 800 L, G 995, G6-amylase, GH13Amy-1, GH13Amy-2, glycogenase, Gt-amy, HaAmy1, HaAmy2, haloalkaline alpha-amylase, HAS, HdAmyI, High pI alpha-amylase, HPA, HSA, HSAmy, HSAmy-ar, htur2110, human salivary alpha-amylase, hyperthermophilic alpha-amylase, Isozyme 1B, Kleistase L 1, KRA, LAMY, liquozyme, LLF-alpha-amylase, Low pI alpha-amylase, MalA, maltogenic amylase, maltohexaose-producing alpha-amylase, maltotriose-producing alpha-amylase, MAmy, Maxamyl, Maxilase, Meiotic expression upregulated protein 30, MJA1, More, N8 alpha-amylase, neutral amylase, Pancreatic alpha-amylase, PFTA, Pivozin, PPA, PPA-I, PPA-II, psychrophilic alpha amylase, Ptyalin, raw-starch-digesting alpha-amylase, RB5AMG_01539, Rbamy5, RBLA, RBSA-1, ROAmy, Ruminococcus bromii intermediary alpha-amylase 5, Saci_1162, salt-tolerant alpha-amylase, ScAmy43, Sfamy, Spitase CP 1, SSO1172, SusG, TAA, TaAmy3, Taka-amylase A, Takatherm, TcAmy, TdAmyA, tergal gland protein-1, TfAmy48, Thermamyl, Thermolase, thermostable alpha-amylase, TO_amyl, Tp-AmyS, TVA II, VAAmy1, VAAmy2, VrAmy, ZSA
ECTree
3.2.1.1 alpha-amylaseAdvanced search results
results (
results (KM Value
KM Value on EC 3.2.1.1 - alpha-amylase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
2.3
2-chloro-4-nitrophenyl alpha-D-maltoheptaoside
-
pH 6.8, 30°C, recombinant AMY1-SBD fusion enzyme
0.45
4,6-ethylidene-[G7]-p-nitrophenyl[G1]-alpha-D-maltoheptaoside
-
37°C, pH 6.0
2.46
4-nitrophenyl alpha-D-maltohexaoside
in 50 mM sodium phosphate buffer (pH 6.5) at 75°C
0.45
2-chloro-4-nitrophenyl alpha-maltotrioside
in presence of 0.1 mM NO3-
0.58
2-chloro-4-nitrophenyl alpha-maltotrioside
in presence of 0.2 mM ClO3-
0.76
2-chloro-4-nitrophenyl alpha-maltotrioside
in presence of 0.01 mM Cl-
1.2
2-chloro-4-nitrophenyl alpha-maltotrioside
in presence of 0.01 mM Br-
0.6
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
pH 5.5, 37°C, mutant T212W
0.669
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
mutant enzyme Y380A
0.67
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
Y380A mutant, pH 5.5, 30°C, kcat/Km: 28 1/s/mg/ml
0.7
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
wild-type enzyme, pH 5.5, 30°C, kcat/Km: 38 1/s/mg/ml
0.724
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
mutant enzyme Y380F
0.735
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
mutant enzyme S378T
0.861
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
mutant enzyme S378P
0.87
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
Y380M mutant, pH 5.5, 30°C, kcat/Km: 39 1/s/mg/ml
0.871
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
mutant enzyme Y380M
0.91
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
H395A mutant, kcat below 10 1/s, pH 5.5, 30°C
1.3
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
pH 5.5, 37°C, mutant Y105W
1.4
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
pH 5.5, 37°C, mutant T212P
1.4
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
Y380A/H395A mutant, pH 5.5, 30°C, kcat/Km: 25 1/s/mg/ml
1.7
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
pH 5.5, 37°C, wild-type enzyme
2
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
pH 5.5, 37°C, mutant T212Y
3.1
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
pH 5.5, 37°C, mutant Y105A/T212W
4.9
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
pH 5.5, 37°C, mutant Y105F
6
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
pH 5.5, 37°C, mutant Y105A/T212Y
10
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
above, pH 5.5, 37°C, mutant Y105A
10
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
Y105A mutant, Km above 10 mM, kcat below 10 1/s, pH 5.5, 30°C
10
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
Y105A/Y380A mutant, Km above 10 mM, pH 5.5, 30°C, kcat/Km: 1300 1/s/mg/ml
10
2-chloro-4-nitrophenyl beta-D-maltoheptaoside
Y105A/Y380M mutant, Km above 10 mM, kcat below 10 1/s, pH 5.5, 30°C
0.01
4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside
double mutant, kcat/Km: L134R/S320A, 680000 1/s/M, 25°C, pH 6.0
0.0113
4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside
mutant S329A, kcat/Km: 740000 1/s/M, 25°C, pH 6.0
0.0117
4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside
mutant L134R, kcat/Km: 710000 1/s/M, 25°C, pH 6.0
0.012
4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside
wild-type, kcat/Km: 770000 1/s/M, 25°C, pH 6.0
0.137
4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside
double mutant, L134R/S320A, kcat/Km: L134R/S320A, 1910000 1/s/M, 37°C, pH 4.5
0.147
4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside
wild-type, kcat/Km: 1420000 1/s/M, 37°C, pH 6.5
0.171
4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside
mutant L134R, kcat/Km: 1350000 1/s/M, 37°C, pH 4.5
0.185
4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside
double mutant, L134R/S320A, kcat/Km: L134R/S320A, 680000 1/s/M, 37°C, pH 5.5
0.186
4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside
mutant S329A, kcat/Km: 1210000 1/s/M, 37°C, pH 4.5
0.19
4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside
double mutant, L134R/S320A, kcat/Km: L134R/S320A, 1100000 1/s/M, 37°C, pH 6.5
0.193
4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside
mutant L134R, kcat/Km: 1070000 1/s/M, 37°C, pH 5.5
0.2
4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside
mutant S329A, kcat/Km: 1230000 1/s/M, 37°C, pH 5.5
0.201
4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside
mutant L134R, kcat/Km: 920000 1/s/M, 37°C, pH 6.5
0.201
4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside
wild-type, kcat/Km: 870000 1/s/M, 37°C, pH 5.5
0.205
4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside
mutant S329A, kcat/Km: 880000 1/s/M, 37°C, pH 6.5
0.281
4,6-ethylidene-[G7]-p-nitrophenyl-[G1]-alpha-D-maltoheptaoside
wild-type, kcat/Km: 140000 1/s/M, 37°C, pH 4.5
0.065
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene
pH not specified in the publication, 25°C
0.13
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene
pH not specified in the publication, 25°C
0.223
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene
pH not specified in the publication, 25°C
0.23
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene
-
25°C, pH 7.2
0.26
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene
pH not specified in the publication, 25°C
0.38
amylose
-
DP 17, malt enzyme, calculated as low molecular weight products
0.45
amylose
-
DP 17, recombinant enzyme, calculated as low molecular weight products
36.4
glycogen
-
from rat liver, calculated as low molecular weight products
51.1
glycogen
-
from oyster, calculated as low molecular weight products
3.37
maltoheptaose
in 50 mM sodium phosphate buffer (pH 6.5) at 75°C
1.69
maltopentaose
in 50 mM sodium phosphate buffer (pH 6.5) at 75°C
1.26
maltotriose
in 50 mM sodium phosphate buffer (pH 6.5) at 75°C
0.06
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside
-
37°C, pH 8.0, F256W mutant
0.27
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside
-
37°C, pH 8.0, wild-type
0.07
soluble starch
in 50 mM sodium phosphate buffer (pH 6.5) at 75°C
-
3.3
starch
-
soluble starch, Merck, calculated as low molecular weight products
4.3
starch
-
soluble starch, Zulkowsky type starch, calculated as low molecular weight products
additional information
amylopectin
Km value is 1.9 ml/ml at 5°C, pH 8.3 and 2.5 mg/ml at 15°C, pH 8.3
additional information
amylopectin
-
Km-value: 10.3 mg/ml, pH 7.0, 50°C
additional information
amylopectin
Km-value: 8.95 mg/ml, 45°C, pH 5.0
additional information
amylose
0.13 mg/ml, wild-type enzyme, pH 5.5, 30°C, kcat/Km: 1300 1/s/mg/ml
additional information
amylose
-
0.13 mg/ml, wild-type enzyme, pH 5.5, 30°C, kcat/Km: 1300 1/s/mg/ml
additional information
amylose
0.19 mg/ml, H395A mutant, pH 5.5, 30°C, kcat/Km: 394 1/s/mg/ml
additional information
amylose
-
0.19 mg/ml, H395A mutant, pH 5.5, 30°C, kcat/Km: 394 1/s/mg/ml
additional information
amylose
0.30 mg/ml, Y380A/H395A mutant, pH 5.5, 30°C, kcat/Km: 543 1/s/mg/ml
additional information
amylose
-
0.30 mg/ml, Y380A/H395A mutant, pH 5.5, 30°C, kcat/Km: 543 1/s/mg/ml
additional information
amylose
0.35 mg/ml, Y380M mutant, pH 5.5, 30°C, kcat/Km: 425 1/s/mg/ml
additional information
amylose
-
0.35 mg/ml, Y380M mutant, pH 5.5, 30°C, kcat/Km: 425 1/s/mg/ml
additional information
amylose
0.36 mg/ml, Y380A mutant, pH 5.5, 30°C, kcat/Km: 264 1/s/mg/ml
additional information
amylose
-
0.36 mg/ml, Y380A mutant, pH 5.5, 30°C, kcat/Km: 264 1/s/mg/ml
additional information
amylose
1.0 mg/ml, Y105A/Y380M mutant, pH 5.5, 30°C, kcat/Km: 56 1/s/mg/ml
additional information
amylose
-
1.0 mg/ml, Y105A/Y380M mutant, pH 5.5, 30°C, kcat/Km: 56 1/s/mg/ml
additional information
amylose
1.2 mg/ml, Y105A/Y380A mutant, pH 5.5, 30°C, kcat/Km: 51 1/s/mg/ml
additional information
amylose
-
1.2 mg/ml, Y105A/Y380A mutant, pH 5.5, 30°C, kcat/Km: 51 1/s/mg/ml
additional information
amylose
1.4 mg/ml, Y105A mutant, pH 5.5, 30°C, kcat/Km: 132 1/s/mg/ml
additional information
amylose
-
1.4 mg/ml, Y105A mutant, pH 5.5, 30°C, kcat/Km: 132 1/s/mg/ml
additional information
amylose
-
Km: 2.34 mg and Vmax: 3.047 mg/min
additional information
amylose
Km-value: 6.17 mg/ml, 45°C, pH 5.0
additional information
Ascaris suum glycogen
-
0.0002 mg/ml, muscle alpha-amylase
-
additional information
Ascaris suum glycogen
-
0.0018 mg/ml, intestine alpha-amylase
-
additional information
additional information
-
Km-values of wild-type and mutant enzymes
-
additional information
additional information
-
Km-values of wild-type and mutant enzymes
-
additional information
additional information
-
Km-values of wild-type and mutant enzymes
-
additional information
additional information
-
Km-values of wild-type and mutant enzymes
-
additional information
additional information
-
Km-values of wild-type and mutant enzymes
-
additional information
additional information
-
Km-values of wild-type and mutant enzymes
-
additional information
additional information
-
0.572 mg/ml for soluble starch
-
additional information
additional information
-
0.39 mg/ml for starch
-
additional information
additional information
-
1.6 mg/ml for starch
-
additional information
additional information
-
4.4 mg/ml for starch
-
additional information
additional information
-
0.362 g/ml for enzyme form alpha-I, 0.379 g/ml for enzyme form alpha-II, 0.542 g/ml for enzyme form alpha-III
-
additional information
additional information
-
2.0 g/l for starch
-
additional information
additional information
-
2.3 mg/ml for soluble starch
-
additional information
additional information
-
1.64 mg/ml for soluble starch
-
additional information
additional information
-
detailed kinetic analysis
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
isothermal titration and microcalorimetric analysis, thermodynamics and kinetics
-
additional information
additional information
kinetics of wild-type and mutant enzymes, overview
-
additional information
additional information
-
thermodynamics and kinetics
-
additional information
additional information
-
thermodynamics and kinetics
-
additional information
additional information
-
thermodynamics and kinetics
-
additional information
additional information
-
thermodynamics and kinetics
-
additional information
additional information
-
0.24 mg/ml for soluble starch, variant enzyme M77
-
additional information
additional information
-
0.34 mg/ml for soluble starch, wild-type enzyme
-
additional information
additional information
-
0.63 mg/ml for soluble starch, variant enzyme 21B
-
additional information
additional information
-
0.8 mg/ml for soluble starch, variant enzyme M111
-
additional information
additional information
-
Km for potato starch in the hydrolysis reaction is between 0.02 and 0.05% starch (w/v), AmyD
-
additional information
additional information
-
KM for soluble starch is 1.1 mg/ml, KM-value for potato starch or ice starch is 0.9 mg/ml
-
additional information
additional information
-
KM for soluble starch is constant at pH 5.0-7.0 and increases greatly with decreasing pH from 5.0 to 2.8 and slightly with increasing pH from 7.5 to 9.2, alpha-amylase PA
-
additional information
additional information
-
Km for soluble starch: 0.43 mg/ml
-
additional information
additional information
-
KM for starch is 2.6 mg/ml
-
additional information
additional information
-
Km value for starch is 1.6 mg/ml
-
additional information
additional information
Km values: about 0.5 mg/ml for soluble starch and 4.45 mg/ml for glycogen at pH 9.5 and 50°C
-
additional information
additional information
-
Km values: about 0.5 mg/ml for soluble starch and 4.45 mg/ml for glycogen at pH 9.5 and 50°C
-
additional information
additional information
-
KM-value for soluble starch is 0.72mg/ml
-
additional information
additional information
-
Km-value for soluble starch: 0.0074 mg/ml
-
additional information
additional information
-
KM-value for starch is 0.97 mg/ml
-
additional information
additional information
-
Km-value: 5.2 mg/ml for soluble starch, 14.46 mg/ml for beta-cyclodextrin, 9.18 mg/ml for gamma-cyclodextrin
-
additional information
additional information
KM-values for amylose: 0.363 mg/ml for mutant enzyme Y380A, 0.351 mg/ml for mutant enzyme Y380M, 0.391 mg/ml for mutant enzyme Y380F, 0.203 mg/ml for mutant enzyme S378P, 0.208 mg/ml for mutant enzyme S378T, 0.19 mg/ml for alpha-amylase 1
-
additional information
additional information
-
KM-values for amylose: 0.363 mg/ml for mutant enzyme Y380A, 0.351 mg/ml for mutant enzyme Y380M, 0.391 mg/ml for mutant enzyme Y380F, 0.203 mg/ml for mutant enzyme S378P, 0.208 mg/ml for mutant enzyme S378T, 0.19 mg/ml for alpha-amylase 1
-
additional information
additional information
reaction products determined by HPLC, kinetic properties of native and purified protein shown
-
additional information
additional information
-
kinetics with different substrates, overview
-
additional information
additional information
kinetics with different substrates, overview
-
additional information
additional information
-
apparent Km is 36 mg/ml using cassava starch as substrate
-
additional information
additional information
Km is 11.0 mg/ml, using soluble potato starch as substrate, at pH 7.0 and 30°C
-
additional information
additional information
-
the Km value for starch is 3 mg/ml at 65°C using potato starch (1% (w/v)) as a substrate in 20 mM Tris-HCl, pH 7.4
-
additional information
additional information
-
the KM values of native and non-glycosylated alpha-amylases are 0.052 and 0.098 mg/ml
-
additional information
additional information
-
activation thermodynamics for kinetic stability of native and modified enzymes, overview
-
additional information
additional information
-
kinetics of recombinant mutant enzymes, overiew
-
additional information
additional information
Michaelis-Menten kinetic analysis
-
additional information
additional information
-
Michaelis-Menten kinetic analysis
-
additional information
additional information
Michaelis-Menten kinetic analysis
-
additional information
additional information
-
Michaelis-Menten kinetic analysis
-
additional information
additional information
apparent Km and Vmax values for the hydrolysis of soluble starch are 4 mg/ml and 1111 units/mg for the wild-type enzyme, and 2 mg/ml and 844.5 units/mg for the mutant enzyme, respectively
-
additional information
additional information
classical Michaelis-Menten kinetics, Km for soluble potato starch is 1.22 mg/ml
-
additional information
additional information
-
classical Michaelis-Menten kinetics, Km for soluble potato starch is 1.22 mg/ml
-
additional information
additional information
-
Km and Vmax values of A4 are 4 mg starch/ml and 0.00074 mmol reducing sugar, respectively
-
additional information
additional information
Km for raw starch is 0.79 mg/ml
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additional information
additional information
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Km for raw starch is 0.79 mg/ml
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additional information
additional information
Km for starch is 2.0 mg/ml and for amylpectin 1.1 mg/ml for isozyme HaAmy2
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additional information
additional information
Km for starch is 2.0 mg/ml and for amylpectin 1.1 mg/ml for isozyme HaAmy2
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additional information
additional information
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Km for starch is 2.0 mg/ml and for amylpectin 1.1 mg/ml for isozyme HaAmy2
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additional information
additional information
Km for starch is 9.2 mg/ml and for amylpectin 1.3 mg/ml for isozyme HaAmy1
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additional information
additional information
Km for starch is 9.2 mg/ml and for amylpectin 1.3 mg/ml for isozyme HaAmy1
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additional information
additional information
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Km for starch is 9.2 mg/ml and for amylpectin 1.3 mg/ml for isozyme HaAmy1
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additional information
additional information
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Km is 0.5 mg/ml and Vmax is 3.571 mmol/ml/mg
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additional information
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Km is 1.7 mg/ml and Vmax is 0.5 mg/ml/min
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additional information
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Km is 1.73 mg/ml and Vmax is 1.24 mg/ml/min
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additional information
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Km is 11.71 mg/ml and Vmax is 0.14 mg/ml/min
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additional information
Wangia sp. C52
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Km is 2.08 mg/ml and Vmax is 1.26 mg/ml/min
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additional information
additional information
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Km is 2.71 mg/ml, pH 7.0, 50°C, kinetics and thermodynamics, overview
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additional information
additional information
Bacillus sp. ALSHL3
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Km is 4.79 mg/ml and Vmax is 0.322 mM/min
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additional information
additional information
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Km is 5.8 mg/ml and Vmax is 0.25 mg/ml/min
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additional information
additional information
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Km of BH072 alpha-amylase is 4.27 mg/ml
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additional information
additional information
Km of soluble starch hydrolysis is 2.29 mg/ml, thermodynamics, overview
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additional information
additional information
Lineweaver-Burk kinetics, Km for raw starch from jackfruit is 5.56 mg/ml
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additional information
additional information
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Lineweaver-Burk kinetics, Km for raw starch from jackfruit is 5.56 mg/ml
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additional information
additional information
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Lineweaver-Burk plot, kinetics and thermodynamics for recombinant chimeric acidic alpha-amylase Ba-Gt-amy, detailed overview. Adsorption kinetics of mutant Ba-Gt-amy and wild-type Ba?amy to 1% raw corn starch. Km and Vmax values of mutant Ba-Gt-amy for soluble starch are 0.8 mg/ml and 10.746 mmol*mg/min, whereas those for wild-type Ba-amy are 1.66 mg/ml and 0.0526 mmol*mg/min, respectively
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additional information
additional information
Lineweaver-Burk plot, kinetics and thermodynamics for recombinant chimeric acidic alpha-amylase Ba-Gt-amy, detailed overview. Adsorption kinetics of mutant Ba-Gt-amy to 1% raw corn starch. Km and Vmax values of mutant Ba-Gt-amy for soluble starch are 0.8 mg/ml and 10.746 mmol * mg/min
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additional information
additional information
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Lineweaver-Burk plot, kinetics and thermodynamics for recombinant chimeric acidic alpha-amylase Ba-Gt-amy, detailed overview. Adsorption kinetics of mutant Ba-Gt-amy to 1% raw corn starch. Km and Vmax values of mutant Ba-Gt-amy for soluble starch are 0.8 mg/ml and 10.746 mmol * mg/min
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additional information
additional information
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Lineweaver-Burk plot, Michaelis-Menten kinetics
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additional information
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Lineweaver-Burke double reciprocal plot
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additional information
Michaelis-Menten kinetic analysis, Km values for amylose and amylopectin of the recombinant His6-tagged enzyme are 9.2 mg/ml and 3.8 mg/ml, respectively, pH 5.0, 20°C
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additional information
additional information
Michaelis-Menten kinetics, Km and Vmax of AmyD-1 in 2.0 M NaCl are 2.8 mg/ml and 21.8 mg*ml/ml, respectively, at 20°C and pH 6.0
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additional information
additional information
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Michaelis-Menten kinetics, Km and Vmax of AmyD-1 in 2.0 M NaCl are 2.8 mg/ml and 21.8 mg*ml/ml, respectively, at 20°C and pH 6.0
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additional information
additional information
Bacillus sp. MB6
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Michaelis-Menten kinetics, Km is 5.45 mg/ml and Vmax is 24.15 mg/ml/min
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additional information
additional information
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Michaelis-Menten kinetics, the purified alpha-amylase reveals Km and Vmax values of 0.5% and 1000 U/mg protein for soluble starch
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additional information
additional information
thermodynamic and kinetic analysis, overview
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additional information
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Vmax value of purified native enzyme is 0.836 mg/ml
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with substrate potato starch the enzyme shows a Km of 6.94 mg/ml and Vmax of 0.27 mg/ml * min
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additional information
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside
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0.0001 mg/ml, intestine alpha-amylase
additional information
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside
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0.0003 mg/ml, muscle alpha-amylase
additional information
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside
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0.22 mg/ml, 37°C, pH 5.3
additional information
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside
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0.37 mg/ml, 37°C, pH 5.3
additional information
potato starch
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free enzyme, Km: 0.4 mg/ml, Vmax: 25 mg starch degraded/ml/min/mg protein, immobilized enzyme, first use, Km: 0.98 mg/ml, Vmax: 23.3 mg starch degraded/ml/min/mg protein, first reuse, Km: 1.75 mg/ml, Vmax: 10.4 mg starch degraded/ml/min/mg protein. The higher Km value and the lower affinity for the substrate caused by diffiusional limitations and steric effects leading to a decrease in the accessibility of substrate to the enzyme active site
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additional information
pullulan
Km-value: 15.4 mg/ml, 45°C, pH 5.0
additional information
rabbit glycogen
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0.0015 mg/ml, intestine alpha-amylase
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additional information
soluble starch
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Km: 1.66 mg/ml, Vmax: 0.0001 mmol/min/ml
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additional information
soluble starch
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Km: 3.8 mg/ml, pH 8.5, 70°C
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additional information
soluble starch
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apparent Km value of 7.1 mg/ml, pH 6, 35°C
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additional information
starch
0.60 mg/ml, double mutant, kcat/Km: 1560 ml/mg/s, L134R/S320A, 25°C, pH 6.0
additional information
starch
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0.60 mg/ml, double mutant, kcat/Km: 1560 ml/mg/s, L134R/S320A, 25°C, pH 6.0
additional information
starch
0.61 mg/ml, mutant S329A, kcat/Km: 1870 ml/mg/s, 25°C, pH 6.0
additional information
starch
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0.61 mg/ml, mutant S329A, kcat/Km: 1870 ml/mg/s, 25°C, pH 6.0
additional information
starch
0.64 mg/ml, mutant L134R, kcat/Km: 1670 ml/mg/s, 25°C, pH 6.0
additional information
starch
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0.64 mg/ml, mutant L134R, kcat/Km: 1670 ml/mg/s, 25°C, pH 6.0
additional information
starch
0.86 mg/ml, wild-type, kcat/Km: 1920 ml/mg/s, 25°C, pH 6.0
additional information
starch
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0.86 mg/ml, wild-type, kcat/Km: 1920 ml/mg/s, 25°C, pH 6.0
additional information
starch
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the KM-value for soluble starch (pH 8.5, 70°C) is 3.8 mg/ml
additional information
starch
KM value is 1.31 g/l at 5°C, 1.36 g/l at 25°C, and 1.42 g/l at 35°C respectively, and pH 9.0
additional information
starch
Km value is 1.79 ml/ml at 5°C, pH 8.3 and ,328 mg/ml at 15°C, pH 8.3
additional information
starch
KM value is 3 g/l at 5°C, 3.3g/l at 25°C, and 4.4 g/l at 35°C respectively, and pH 9.0
additional information
starch
Km-value: 16.0 mg/ml, 45°C, pH 5.0
