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Literature summary for 3.2.1.1 extracted from

  • Dong, G.; Vieille, C.; Savchenko, A.; Zeikus, J.
    Cloning, sequencing, and expression of the gene encoding extracellular alpha-amylase from Pyrococcus furiosus and biochemical characterization of the recombinant enzyme (1997), Appl. Environ. Microbiol., 63, 3569-3576.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Pyrococcus furiosus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not require Ca2+ for activity Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
2 * 66000, SDS-PAGE Pyrococcus furiosus
100000
-
gel filtration Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus O08452
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3900
-
pH 6.0, at 98°C Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O liquefying enzyme. The main products of polysaccharide hydrolysis are G2 to G7. A small amount of G1 is formed after long hydrolysis periods. The enzyme A hydrolyzes long-chain oligosaccharides faster than shorter chain oligosaccharides Pyrococcus furiosus ?
-
?
amylose + H2O liquefying enzyme. The main products of polysaccharide hydrolysis are G2 to G7. A small amount of G1 is formed after long hydrolysis periods. The enzyme A hydrolyzes long-chain oligosaccharides faster than shorter chain oligosaccharides Pyrococcus furiosus ?
-
?
glycogen + H2O liquefying enzyme. The main products of polysaccharide hydrolysis are G2 to G7. A small amount of G1 is formed after long hydrolysis periods. The enzyme A hydrolyzes long-chain oligosaccharides faster than shorter chain oligosaccharides Pyrococcus furiosus ?
-
?
additional information does not hydrolyze pullulan, cyclodextrins, sucrose Pyrococcus furiosus ?
-
?
soluble starch + H2O liquefying enzyme. The main products of polysaccharide hydrolysis are G2 to G7. A small amount of G1 is formed after long hydrolysis periods. The enzyme A hydrolyzes long-chain oligosaccharides faster than shorter chain oligosaccharides Pyrococcus furiosus ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 66000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
no activity at room temperature Pyrococcus furiosus
98
-
assay at Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
98
-
half-life: 13 min Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
Pyrococcus furiosus
6
-
assay at Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
4.7 7 80% activity or more between pH 4.7 and pH 7.0 Pyrococcus furiosus