Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pyrococcus furiosus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require Ca2+ for activity | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
2 * 66000, SDS-PAGE | Pyrococcus furiosus |
100000 | - |
gel filtration | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | O08452 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3900 | - |
pH 6.0, at 98°C | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | liquefying enzyme. The main products of polysaccharide hydrolysis are G2 to G7. A small amount of G1 is formed after long hydrolysis periods. The enzyme A hydrolyzes long-chain oligosaccharides faster than shorter chain oligosaccharides | Pyrococcus furiosus | ? | - |
? | |
amylose + H2O | liquefying enzyme. The main products of polysaccharide hydrolysis are G2 to G7. A small amount of G1 is formed after long hydrolysis periods. The enzyme A hydrolyzes long-chain oligosaccharides faster than shorter chain oligosaccharides | Pyrococcus furiosus | ? | - |
? | |
glycogen + H2O | liquefying enzyme. The main products of polysaccharide hydrolysis are G2 to G7. A small amount of G1 is formed after long hydrolysis periods. The enzyme A hydrolyzes long-chain oligosaccharides faster than shorter chain oligosaccharides | Pyrococcus furiosus | ? | - |
? | |
additional information | does not hydrolyze pullulan, cyclodextrins, sucrose | Pyrococcus furiosus | ? | - |
? | |
soluble starch + H2O | liquefying enzyme. The main products of polysaccharide hydrolysis are G2 to G7. A small amount of G1 is formed after long hydrolysis periods. The enzyme A hydrolyzes long-chain oligosaccharides faster than shorter chain oligosaccharides | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 66000, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
no activity at room temperature | Pyrococcus furiosus |
98 | - |
assay at | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
98 | - |
half-life: 13 min | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | - |
Pyrococcus furiosus |
6 | - |
assay at | Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.7 | 7 | 80% activity or more between pH 4.7 and pH 7.0 | Pyrococcus furiosus |