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Literature summary for 3.2.1.1 extracted from
- An amylase producing maltotriose from Bacillus subtilis (1985), Agric. Biol. Chem., 49, 1091-1097.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 5 mM AgNO3, 82% inhibition | Bacillus subtilis |  |
| Ba2+ | 5 mM BaCl2, 28% inhibition | Bacillus subtilis | |
| Co2+ | 5 mM, 16% inhibition | Bacillus subtilis | |
| Cu2+ | 5 mM CuSO4, 16% inhibition | Bacillus subtilis | |
| Fe2+ | 5 mM FeSO4, 71% inhibition | Bacillus subtilis | |
| Hg2+ | 5 mM HgCl2, 98% inhibition | Bacillus subtilis | |
| Ni2+ | 5 mM NiCl2, 52% inhibition | Bacillus subtilis | |
| Sr2+ | 5 mM SrCl2, 36% inhibition | Bacillus subtilis | |
| Zn2+ | 5 mM ZnSO4, 95% inhibition | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
gel filtration | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Bacillus subtilis | additional information | maltotriose | ? | |
| amylose + H2O | short chain, DP 17 | Bacillus subtilis | additional information | yield of maltotriose is 69% | ? | |
| glycogen + H2O | - |
Bacillus subtilis | additional information | maltotriose | ? | |
| starch + H2O | soluble starch | Bacillus subtilis | additional information | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | 50 | 23°C: about 50% of maximal activity, 50°C: about 30% of maximal activity | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
unstable above | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | pH 5.0: about 65% of maximal activity, pH 8.0: about 55% of maximal activity | Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | 25°C, 5 h, stable | Bacillus subtilis |
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