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Literature summary for 3.2.1.1 extracted from
- Multiple components of alpha-amylase in germinating tubers of a yam, Dioscorea dumetorum (1980), J. Biochem., 87, 289-295.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 0.362 g/ml for enzyme form alpha-I, 0.379 g/ml for enzyme form alpha-II, 0.542 g/ml for enzyme form alpha-III | Dioscorea dumetorum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
enzyme form alpha-II, gel filtration | Dioscorea dumetorum |
| 48500 | - |
enzyme form alpha-III, gel filtration | Dioscorea dumetorum |
| 52000 | - |
enzyme form alpha-I, gel filtration | Dioscorea dumetorum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dioscorea dumetorum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| three distinct amylolytically active protein forms | Dioscorea dumetorum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| tuber | germinating | Dioscorea dumetorum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Dioscorea dumetorum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | - |
Dioscorea dumetorum | additional information | one enzyme form produces maltose and maltohexaose, another form produces exclusively maltopentaose from polysaccharide substrates | ? |  |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
enzyme form alpha-II | Dioscorea dumetorum |
| 60 | - |
enzyme form alpha-I anf alpha-III | Dioscorea dumetorum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | 5.9 | enzyme form alpha-II | Dioscorea dumetorum |
| 5.8 | 6.1 | enzyme form alpha-I | Dioscorea dumetorum |
| 6.1 | 6.2 | enzyme form alpha-III | Dioscorea dumetorum |
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Release 2023.1 (January 2023)
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