Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | calcium stabilizes the conformation of alpha-amylase and also involved in substrate binding, but inhibits enzyme activity | Glycine max | |
Co2+ | - |
Glycine max | |
EDTA | - |
Glycine max | |
Mg2+ | - |
Glycine max | |
Zn2+ | - |
Glycine max |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | calcium stabilizes the conformation of alpha-amylase and also involved in substrate binding, but inhibits enzyme activity | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84000 | - |
gel filtration | Glycine max |
100000 | - |
1 * 100000, SDS-PAGE | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Glycine max | soybean alpha-amylase shows high specificity for its primary substrate starch, followed by amylopectin and dextrin (III), lower activity with amylopectin, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 400fold from germinating seeds to homogeneity by ammonium sulfate fractionation, heat treatment at 70°C for 10 min, beta-cyclodextrin affinity chromatography, and gel filtration | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | germinating | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
384.6 | - |
purified enzyme, pH 5.5, temperature not specified in the publication | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | soybean alpha-amylase shows high specificity for its primary substrate starch, followed by amylopectin and dextrin (III), lower activity with amylopectin, overview | Glycine max | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 100000, SDS-PAGE | Glycine max |
More | peptide mapping, overview | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
More | the soybean alpha-amylase belongs to glycosyl hydrolase family 13 | Glycine max |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 85 | activity range | Glycine max |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
thermal inactivation, first-order kinetics with rate constant equal to 0.0063/min | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Glycine max |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Glycine max | MALDITOF analysis | - |
4.85 |