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Literature summary for 3.2.1.1 extracted from
- Purification and characterization of alpha-amylases from the intestine and muscle of Ascaris suum (Nematoda) (2001), Acta Biochim. Pol., 48, 763-774.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-phosphoglyceric acid | 50 mM, 7fold activation of muscle alpha-amylase | Ascaris suum |  |
| 3-Phosphoglyceric acid | 50 mM, 5fold activation of muscle alpha-amylase | Ascaris suum | |
| CaCl2 | slight activation of muscle and intestine alpha-amyloamylase | Ascaris suum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 0.05 mM, 64% inhibition of intestine alpha-amylase, complete inhibition of muscle alpha-amylase | Ascaris suum | |
| glycerol | 0.05 mM, 68% inhibition of intestine alpha-amylase | Ascaris suum | |
| iodoacetic acid | 0.05 mM, complete inhibition of intestine and muscle alpha-amylase | Ascaris suum | |
| p-chloromercuribenzoate | 0.05 mM, 52% inhibition of intestine alpha-amylase, 68% inhibition of muscle alpha-amylase | Ascaris suum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside | 0.0001 mg/ml, intestine alpha-amylase | Ascaris suum | |
| additional information | - |
Ascaris suum glycogen | 0.0002 mg/ml, muscle alpha-amylase | Ascaris suum | |
| additional information | - |
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside | 0.0003 mg/ml, muscle alpha-amylase | Ascaris suum | |
| additional information | - |
starch | 0.0005 mg/ml, intestine alpha-amylase | Ascaris suum | |
| additional information | - |
rabbit glycogen | 0.00054 mg/ml, muscle alpha-amylase | Ascaris suum | |
| additional information | - |
amylopectin | 0.0012 mg/ml, intestine alpha-amylase | Ascaris suum | |
| additional information | - |
rabbit glycogen | 0.0015 mg/ml, intestine alpha-amylase | Ascaris suum | |
| additional information | - |
Ascaris suum glycogen | 0.0018 mg/ml, intestine alpha-amylase | Ascaris suum | |
| additional information | - |
amylose | 0.002 mg/ml, intestine alpha-amylase | Ascaris suum | |
| additional information | - |
amylopectin | 0.002 mg/ml, muscle alpha-amylase | Ascaris suum | |
| additional information | - |
starch | 0.003 mg/ml, muscle alpha-amylase | Ascaris suum | |
| additional information | - |
amylose | 0.004 mg/ml, muscle alpha-amylase | Ascaris suum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | isoenzymes alpha-I1 and alpha-I2 and muscle alpha-amylase | Ascaris suum | 5764 | - |
| microsome | muscle alpha-amylase | Ascaris suum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
x * 53000, SDS-PAGE, intestine alpha-amylase | Ascaris suum |
| 74000 | - |
x * 74000, SDS-PAGE, isoenzyme alpha-I1 | Ascaris suum |
| 83000 | - |
x * 83000, SDS-PAGE, isoenzyme alpha-I2 | Ascaris suum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Ascaris suum | intestine and muscle alpha-amylase | ? | - |
? | |
| amylose + H2O | Ascaris suum | intestine and muscle alpha-amylase | ? | - |
? | |
| glycogen + H2O | Ascaris suum | glycogen from Ascaris suum, intestine and muscle alpha-amylase | ? | - |
? | |
| starch + H2O | Ascaris suum | intestine and muscle alpha-amylase | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ascaris suum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, 50°C denaturation, P-cellulose, PAGE, muscle alpha-amylase, 50°C denaturation, DEAE-cellulose, P-cellulose, intestine alpha-amylase | Ascaris suum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| intestine | isoenzymes alpha-I1 and alpha-I2 | Ascaris suum | - |
| muscle | - |
Ascaris suum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.94 | - |
muscle alpha-amylase | Ascaris suum |
| 7.7 | - |
intestine alpha-amylase | Ascaris suum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | intestine and muscle alpha-amylase | Ascaris suum | ? | - |
? | |
| amylose + H2O | intestine and muscle alpha-amylase | Ascaris suum | ? | - |
? | |
| glycogen + H2O | glycogen from Ascaris suum, intestine and muscle alpha-amylase | Ascaris suum | ? | - |
? | |
| glycogen + H2O | glycogen from rabbit or Ascaris suum, intestine and muscle alpha-amylase | Ascaris suum | ? | - |
? | |
| p-nitrophenyldi[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside + H2O | - |
Ascaris suum | p-nitrophenol + p-nitrophenyl-alpha-D-glucopyranoside + p-nitrophenyldi[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside + p-nitrophenyltri[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside | intestine alpha-amylase | ? | |
| p-nitrophenyldi[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside + H2O | - |
Ascaris suum | p-nitrophenyl-alpha-D-glucopyranoside + p-nitrophenyldi[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside + p-nitrophenyltri[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside | muscle alpha-amylase | ? | |
| p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside + H2O | - |
Ascaris suum | p-nitrophenol + p-nitrophenyl-alpha-D-glucopyranoside + p-nitrophenyldi[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside + p-nitrophenyltri[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside + p-nitrophenyltetra[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside | intestine and muscle alpha-amylase | ? | |
| p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside + H2O | - |
Ascaris suum | p-nitrophenyl-alpha-D-glucopyranoside + p-nitrophenyldi[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside + p-nitrophenyltri[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside + p-nitrophenyltetra[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside | muscle alpha-amylase | ? | |
| starch + H2O | intestine and muscle alpha-amylase | Ascaris suum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, SDS-PAGE, intestine alpha-amylase | Ascaris suum |
| ? | x * 74000, SDS-PAGE, isoenzyme alpha-I1 | Ascaris suum |
| ? | x * 83000, SDS-PAGE, isoenzyme alpha-I2 | Ascaris suum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
2 maxima, muscle alpha-amylase | Ascaris suum |
| 40 | - |
2 maxima, intestine alpha-amylase | Ascaris suum |
| 50 | - |
2 maxima, intestine alpha-amylase, muscle alpha-amylase | Ascaris suum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 60 | muscle alpha-amylase, approx. 50% of maximal activity at 15°C, 20% of maximal activity at 60°C | Ascaris suum |
| 20 | 60 | intestine alpha-amylase, approx. 18% of maximal activity at 20°C, approx. 20% of maximal activity at 60°C | Ascaris suum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
muscle alpha-amylase, 50% loss of activity, intestine alpha-amylase, 90% loss of activity | Ascaris suum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
intestine alpha amylase | Ascaris suum |
| 8.4 | - |
muscle alpha-amylase | Ascaris suum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.2 | intestine alpha-amylase, approx. 20% of maximal activity at pH 5.5, approx. 40% of maximal activity at pH 9.2 | Ascaris suum |
| 6.5 | 10.4 | 10% of maximal activity at pH 6.5, approx. 30% of maximal activity at pH 10 | Ascaris suum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Ascaris suum | isoelectric focusing, isoenzyme alpha-I1 | - |
4.5 |
| Ascaris suum | isoelectric focusing, isoenzyme alpha-I2 | - |
4.9 |
| Ascaris suum | isoelectric focusing, muscle alpha-amylase | - |
5 |
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